ABSTRACT
The paper expatiates on the study situation of medical monitoring system from the aspects like mobile medical,physiological index detection equipment,monitoring system at medical institution level and household monitoring system.It analyzes study hotspots of medical monitoring system from the three aspects of cloud platform of health monitoring,wearable medical devices and body sensor network.It also points out problems in medical monitoring system based on Internet of things and predicts its future development.
ABSTRACT
Focusing on the problems in the major of Intemet of Things (IoT) engineering in some schools,such as lack of industry positioning and indefinite cultivating objectives,the paper puts forward the major building of IoT engineering oriented to the medical industry in cultivating objectives,requirements,direction setting,curriculum system,practical link setting,etc.,discusses the building of relevant laboratories and practical training bases,and introduces the functions and requirements of various laboratories.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of Wnt signaling suppression on proliferation of non small cell lung cancer to gefitinib, and its related mechanisms.</p><p><b>METHODS</b>PC9 and PC9/AB2 cells of both gefitinib sensitive and resistant were treated with different concentrations of gefitinib, and the proliferation index was measured using CCK8 kit. The members of Wnt signaling pathway were detected by Western blot. Dual luciferase reportor gene assay (TOP Flash) was used to document the transcriptional level of β-catenin. β-catenin siRNA was transfected into PC9/AB2 cells to suppress the Wnt signaling transcription, followed by treatment with different concentrations of gefitinib. Western blot was then used to detect the expression of EGFR and its downstream signaling after inhibit the expression of β-catenin.</p><p><b>RESULTS</b>Treating with different concentrations of gefitinib, the resistance of PC9/AB2 cells to gefitinib was significantly increased (P < 0.05). The members of Wnt signaling expressed at higher level in PC9/AB2 cells than in PC9 cells (t = 24.590, P = 0.000). TOP Flash examination showed that the endogenous transcriptional activity of Wnt signaling was higher in PC9/AB2 cell than that in PC9 cell (t = 4.983, P = 0.008). Compared with the negative control group, apoptotic rate and sensitivity to gefitinib significantly increased in interfered group (P < 0.05). The expression of p-ERK1/2 significantly decreased after Wnt signaling suppression, although other proteins showed no significant alterations.</p><p><b>CONCLUSION</b>Suppressing the activity of Wnt signaling can partly reverse the celluar resistance to gefitinib in non small cell lung cancer.</p>