Construction of the dioxin bioassay method based on the clonal expressed aryl hydrocarbon receptor system / 中华预防医学杂志

<p><b>OBJECTIVE</b>To study the specific binding of the artificial clonal aryl hydrocarbon receptor translocator (ARNT) with the natural aryl hydrocarbon receptor (AhR) and the recolonization by polyclonal antibody. The dose-response relationship with tetrachlo-rodibenzo-dioxin (TCDD) was also studied to develop TCDD detection method and the binding degree related to dose response.</p><p><b>METHODS</b>(1) The target genes including AhR-PAS, AhR-C and ARNT-PAS were amplified by RT-PCR by using the total RNA purified from the liver cells of C57BL/6J mice as templates to construct pGEX-5X1 recombinants. The recombinant plasmids were expressed in E. coli. (2) The rabbits were immuned by the clonal fusion proteins: AhR-PAS, AhR-C to prepare the polyclonal antibody. (3) The natural AhR from the hepatic cytosol of C57BL/6J mice was extracted. The artificial cloning expressed fusion protein:GST-ARNT-PAS and the natural AhR were incubated in different dose of TCDD. The quantity of the heterodimer through affinity adsorption and Western blots were measured.</p><p><b>RESULTS</b>(1) The target proteins including AhR-PAS, AhR-C and ARNT-PAS were successfully cloned and expressed in E. coli. (2) The detection limit of polyclonal antibody AhR-PAS and AhR-C were 5 ng and 1 ng, respectively. (3) The total protein concentration prepared from the liver cells was 60.5 mg/ml. The artificial clonal protein ARNT-PAS could specifically bind to the natural AhR complex with the existence of TCDD. The detection limit of TCDD was 0.25 pmol which was 80 pg approximately.</p><p><b>CONCLUSION</b>A TCDD detection method based on the aryl hydrocarbon receptor system was established and the detection limit might reach pg grade.</p>

Investigation on nutritional intakes for hospitalized children with blood disease / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To investigate the diet and nutritional status of hospitalized children with blood disease in order to provide nutritional guidelines.</p><p><b>METHODS</b>The patients' daily dietary intakes, including breakfast, lunch, dinner and additional meals, were recorded in detail for seven consecutive days. The intake amount of various nutrients was calculated using the dietary database.</p><p><b>RESULTS</b>The majority of children with blood disease showed inadequate intakes of calories [mean 1825.81 kCal/d, 73.62% of the recommended intake (RNI)] and protein (mean 67.68 g/d, 81.34% of RNI). Intakes of vitamin E and riboflavin were adequate, but intakes of vitamin A, thiamine and vitamin C (66.67%, 77.78% and 69.89% of RNI, respectively) were inadequate. Iron and selenium intakes were adequate, but calcium and zinc intakes (41.11% and 56.21% of RNI, respectively) were grossly inadequate.</p><p><b>CONCLUSIONS</b>Hospitalized children with blood disease had decreased dietary intakes of calories, protein, vitamin A, vitamin C, thiamin, calcium and zinc. The dietary pattern and nutritional intake need to be improved.</p>

Study on content of iodine in food in Tianjin market and iodine nutrition conditions among college students / 中华预防医学杂志

<p><b>OBJECTIVE</b>To know about content of iodine in foods sold in Tianjing markets presently, and the iodine nutrition conditions in college students. It was also aimed to probe the functions of the iodized salt complement with the dietary iodine intake, and whether the urine iodine could reflect dietary iodine intake.</p><p><b>METHODS</b>278 food samples in markets were collected by a randomly stratified sampling method, while the arsenic-cerium catalytic contact method was used to determine the content in food. The dietary information of students for seven days was recorded, and the urine iodine was determined through the arsenic-cerium catalytic spectrophotometry.</p><p><b>RESULTS</b>The determination of 47 kinds and 278 food samples indicated that the content of iodine within animal foods (7.8 microg/100 g - 30.8 microg/100 g) was higher than that within plant foods (1.8 microg/100 g - 16.1 microg/100 g). The investigation also showed that students who regarded vegetarian food as principle accounted for 70. 19%. The amount of dietary iodine intake among those students, based on the dietary survey, was (111.67 +/- 53.18) microg/d, while supplementary iodine from iodized salt was about (230.27 +/- 45.55) microg/d. Therefore, the total iodine provided from diet would be (341.95 +/- 89.58) microg/d. Modified by urine creatinine, the median of urine iodine was 271.28 microg/gCr, and the urine iodine and dietary iodine intake was found positively related (r(s) = 0.463, P < 0.01).</p><p><b>CONCLUSIONS</b>Regarding the vegetarian food as the principle, most of students investigated are not rich. The dietary iodine intake is lower than RDA (150 microg), but it can be obtained the iodized salt by 230. 27 microg, which is the possible supplement to the shortage from foods.</p>

Central giant cell lesions of the jaws: a clinicopathological study of 31 cases / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To study the clinicopathologic features of central giant cell granuloma (CGCG) of the jaws and the relationship between the pathologic features and its clinical behavior.</p><p><b>METHODS</b>Histologic, radiographic and follow-up information for 31 cases of central giant cell granuloma were reviewed. The histopathologic patterns were analyzed between nonrecurrent and recurrent cases for which the following-up information was available.</p><p><b>RESULTS</b>The majority of the giant cell granuloma of the jaws occurred in patients under 30 with a predilection of females and mostly were involved in the mandible. The radiographic features of CGCG non-specific. The multinucleated giant cell scattered unevenly, the numbers of the nuclei were few and mostly 10-19. The marked fibrosis, the multiple area of hemorrhage, abundant hemosiderin and newly formed bone were always present in the lesions. No significant difference exited between the recurrence and nonrecurrence groups in the pathologic features. The patients with aggressive behavior showed more consistent with the recurrence.</p><p><b>CONCLUSIONS</b>CGCG was a non-neoplastic lesion of the jaws which was different from the giant cell tumor. It was difficult to distinguish between the CGCG and giant cell tumor (GCT), and to predict its clinical behavior only by the histopathological patterns. It was helpful to combine the clinical presentation of CGCG with its treatment.</p>

Clinicopathologic study of a family cherubism / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the clinicopathologic features of familial cherubism and its differentiation from other giant cell lesions in jaws and the results of surgical treatments with a long-term follow-up.</p><p><b>METHODS</b>Four cases of familial cherubism were reviewed and their clinical and radiographic features, histopathologic appearance, biochemical markers and surgical treatments analysed.</p><p><b>RESULTS</b>Clinically, cherubism was characterized by bilateral painless swelling of jaws, mandibular deformity was common. Radiographs showed multilocular radiolucencies with sclerotic thickening border. Histopathologically, numerous randomly distributed multinucleated giant cells and vascular spaces within a fibrous connective tissue stroma with or without eosinophilic collagen perivascular cuffing were shown. The lesion regressed without treatment in 1 cases. Curettage was performed in 3 cases with good results.</p><p><b>CONCLUSIONS</b>Cherubism can be diagnosed according to its typical clinical and radiographical features with a positive family history. It might regress without treatment. But surgery intervention is suggested to improve physiological function and to solve the psychologic problem of the patients.</p>