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BACKGROUND:Hepatoblasts are one of high-grade cell sources for severe liver disease treatment.Efficient separation and purification of hepatoblasts from fetal liver has important practical significances.OBJECTIVE:To introduce the preparation of hepatoblasts from fetal liver,conduct a transversal comparison of these preparation methods,and point out their advantages and shortcomings,so as to provide the evidences for the experimental and clinical choices of hepatoblasts.RETRIEVAL STRATEGY:Using the keywords of "fetal liver progenitor/hepatoblasts,isolation/purification",we searched the articles about fetal liver hepatoblasts separation and purification in PUBMED between January 1997 and August 2007 in English.At the same time,we searched the relative articles in CNKI using the keywords of "liver,embryo,endothelial progenitor/hepatoblasts" between January 1997 and August 2007 in Chinese.In addition,some related books and foreign articles were checked in the laboratory of Jilin University.After the first examination,the articles published in the authority magazines within five years were given prior consideration.Exclusive criteria were those repetitive researches or Meta-analysis.Totally 245 articles were collected,we selected 33 representative articles about hepatoblasts separation and purification methods.LITERATURE EVALUATION:Among 31 included articles,10 ones introduced the study background,5 ones studied the fetal liver cell dissociation,9 ones referred to the hepatoblasts separation and purification,and 7 ones indicated the problems and prospect in this field.DATA SYNTHESIS:The hepatic epithelial cell precursor cells(also known as hepatoblasts) with hepatic and biliary epithelial cell differentiation potential isolated from fetal liver,could in vitro proliferate and differentiate,after transplantation in vivo they show a good homing,and the ability to integrate and regenerate.Hepatoblasts isolation technology is being improved daily,and the related researches on this field will boost cell-based liver therapy in clinical application.CONCLUSION:Improvements and combination of the various isolation methods,as well as new and high-specific antigen discovery will further improve the isolation efficiency of hepatoblasts from fetal liver.
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Objective To investigate the expression of a novel imprinted gene,PEG10,in human gastric adenocarcinoma,and the effect of PEG10 on cell growth and proliferation of gastric cancer cells.Methods The PEG10 mRNA expressions in 40 human gastric adenocarcinoma,the corresponding adjacent normal tissues and 6 nomal gastric tissues were detected by RT-PCR.The expression vectors of PEG10 were constructed and transfected into gastric cancer cell line MKN45 which had no endogenetic PEG10 expression.Cell growth ability was measured by MTT assay.Results High PEG10 mRNA expression level was detected in 9 of 20(45.0%) human gastric adenocarcinoma which was significantly higher than those of the matched normal tissues(10.0%)(P
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<p><b>OBJECTIVES</b>To study the interaction of beta-2-glycoprotein I (beta 2GPI) with the membrane of hepatocytes and determine whether beta 2GPI participates in HBV infection.</p><p><b>METHODS</b>Ligand blotting, fluorescence microscopy, and fluorescence activated cell sorter (FACS) analysis were used to detect the specific interaction of beta 2GPI with the hepatoma cell line smmc7721, the gastric carcinoma cell line SGC7901, and the lymphoma cell line HL-60.</p><p><b>RESULTS</b>A specific 40 kDa beta 2GPI band was observed by ligand blotting in the case of smmc7721 cells. No such band was observed in SGC7901 or HL-60 cells. Fluorescence microscopy also revealed specific binding of FITC-beta 2GPI to smmc7721 cells, but neither to SGC7901 nor HL-60 cells. FACS analysis demonstrated that the binding rate of FITC-beta 2GPI to smmc7721 cells was significantly higher than these in SGC7901 and HL-60 cells (P < 0.01). The binding rate to smmc7721 cells did not increase with increasing amounts of FITC-beta 2GPI.</p><p><b>CONCLUSIONS</b>There is a specific beta 2GPI-binding protein on the membrane of hepatoma cells in cell line smmc7721 which as the beta 2GPI receptor may participate in HBV infection of hepatocytes.</p>
Subject(s)
Animals , Carcinoma, Hepatocellular , Cell Membrane , Metabolism , Flow Cytometry , Glycoproteins , Metabolism , Hepatocytes , Metabolism , Liver Neoplasms, Experimental , Metabolism , Platelet Glycoprotein GPIb-IX Complex , Platelet Membrane Glycoproteins , Tumor Cells, Cultured , beta 2-Glycoprotein IABSTRACT
Objective To study the relationship between anti-? 2-glycoprotein I(a? 2GPⅠ) and extracellular matrix (ECM) in patients with post-hepatitis B cirrhosis (PHBC).Method The of patients with chronic hepatitis B (CHB) and PHBC were studied for the serum a? 2GPⅠ levels by ELISA with purified ? 2GPⅠ as antigen, and we investigated serum level of collagen IV (IV-C)?hyaluronic acid (HA) and laminin (LN) in these patients.Result High positive rate was observed compared with control group (P
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Objective To investigate the relationship between some autoantibodies including anti-? 2-glycoprotein Ⅰ (anti-? 2GPⅠ) and chronic liver disease. Methods A cohort of patients with chronic hepatitis B (CHB) and post-hepatitis B cirrhosis (PHBC) was studied for the serum anti-? 2GPⅠ levels by ELISA with purified ? 2GPⅠ as antigen. The serum dsDNA, smooth muscle antibody (SMA) and ribonucleoprotein antibody (RNPA) were also detected. Results High positive rate was observed in patients with CHB or PHBC (20.9%, 9/43; 49.3%,35/75) comparing with that in control group (3.1%, 1/32) ( P