ABSTRACT
INTRODUCTION: Heat shock protein70 (HSP70) is a highly conserved family of proteins produced after a variety of stresses. Many studies reported that the overexpression of HSP70 can improve the prognosis of the patients with sepsis through a reduction of the nitric oxide concentration. However, these results only revealed the effect of HSP70 and nitric oxide. No studies have examined the relationship between HSP70 and nitric oxide. The aim of this study was to evaluate the effect of the overexpression of HSP70 on the expression of inducible nitric oxide synthase and the nitric oxide concentration. In addition, the mechanism of the relationship of HSP70 and inducible nitric oxide synthase (iNOS) in sepsis was examined. MATERIALS AND METHODS: The experiments were performed on male sprague-dawley rats. Sepsis was induced by a cecal ligation and puncture (CLP). Glutamine (GLN) or saline was administered 1 hour after the initiation of sepsis. Serum and lung tissues were acquired from the rats 12 hours or 24 hours after the initiation of sepsis. The nitric oxide concentration, the expression of HSP70 in lung, and the gene expression of iNOS in lung were analyzed. The three groups, sham operation, CLP and CLP+GLN, were compared. RESULTS: Compared to the other groups, in CLP+GLN, GLN administered after the initiation of sepsis enhanced the expression of HSP70 in the lung at 12 hours (47.19+/-10.04 vs. 33.22+/-8.28, P=0.025) and 24 hours (47.06+/-10.60 vs. 31.90+/-4.83, P=0.004). In CLP+GLN, GLN attenuated the expression of iNOS messenger RNA (mRNA) in the lung at 12 hours (5,513.73+/-1,051.60 vs. 4,167.17+/-951.59, P=0.025) and 24 hours (18,740.27 +/-8,241.20 vs. 9,437.65+/-2,521.07, P=0.016), and reduced the concentration of nitric oxide in the serum at 12 hours (0.86+/-0.48 vs. 3.82+/-2.53, P=0.016) and 24 hours (0.39+/-0.25 vs. 1.85+/-1.70, P=0.025). CONCLUSION: The overexpression of HSP70 induced by the administration of GLN in sepsis attenuates the expression of the iNOS gene but reduces the nitric oxide concentration.
Subject(s)
Animals , Humans , Male , Rats , Gene Expression , Glutamine , Heat-Shock Proteins , Hot Temperature , HSP70 Heat-Shock Proteins , Ligation , Lung , Nitric Oxide , Nitric Oxide Synthase , Nitric Oxide Synthase Type II , Prognosis , Proteins , Punctures , Rats, Sprague-Dawley , RNA, Messenger , Salicylamides , Sepsis , ShockABSTRACT
Infections of the oral and maxillofacial region are one of the most common conditions for which a patient presents to a maxillofacial surgeons. Although these infections can arise from a variety of source, dental disease is the most common etiology. So, odontogenic infection are frequently encountered in the practice of oral and maxillofacial surgery. These infections often respond to antimicrobial chemotherapy or surgical intervention, such as extraction of teeth, incision and drainage through clinical features. But, odontogenic infections have the potential to spread via the fascial spaces in the head and neck region, and, they spread to cavernous sinus, deep musculofascial space and other vital structure. We have undertaken clinical studies on infections in the oral and maxillofacial regions by analyzing retrospectively hospitalized patients in the Department of Oral and Maxillofacial Surgery, Chonbuk National University Hospital past 5 years from 2000 to 2004. And, the patients'age, sex, medical history, causes of the infection, surgical intervention, and other clinical parameters were reviewed. The obtained results were as follows: 1. The most frequent cause of oral and maxillofacial infection was odontogenic. And in the odontogenic cause, dental caries was the most common cause (47.2%). 2. The most common fascial space involved was the submandibular space (15.7%), followed by the buccal space (14.8%). 3. 60.4% of all patients required surgical drainage of the abscess, endodontic treatment or tooth extraction or periodontal treatment with drainage. 4. The most causative organism isolated from the pus culture were streptococcus viridans (53.9%). 5. Underlying medical problems were found in 136 patients (41.9%), the most common being hypertension (27.9%) and diabetes (14.7%).
Subject(s)
Humans , Abscess , Cavernous Sinus , Dental Caries , Drainage , Drug Therapy , Head , Hypertension , Neck , Retrospective Studies , Stomatognathic Diseases , Suppuration , Surgery, Oral , Tooth , Tooth Extraction , Viridans StreptococciABSTRACT
We evaluated the need for prophylactic postoperative oral antibiotic medication in extraction of asymptomatic impacted mandibular third molars. All patient didn't show sign of pain, inflammation, swelling and trismus at the time of extraction. In the experimental group, oral antibiotic medication(Amoxicillin) was carried out for 5 days postoperatively. In the control group, the patients received no antibiotic medication. All groups didn't use antibiotic irrigation solution. Rule of group composition randomized. The surgical technique was the same in all cases. Parameters that were evaluated were infection, pain, facial swelling, trismus. We could not find any significant difference between the experimental and control groups.(P<0.05) The results of our study show that post operative oral prophylactic antibiotic medication after the extraction of impacted mandibular third molars does not contribute to less infection, pain, facial swelling and increased mouth opening after surgery. Therefore we suggest that prophylactic postoperative oral antibiotic medication is not needed in extraction of asymptomatic impacted mandibular third molars.
Subject(s)
Humans , Facial Pain , Inflammation , Molar, Third , Mouth , TrismusABSTRACT
Subject(s)
Female , Humans , Male , Age Distribution , Dentistry , Dentofacial Deformities , Diagnosis , Facial Asymmetry , Genioplasty , Inpatients , Orthognathic Surgery , Osteotomy , Osteotomy, Sagittal Split Ramus , Prognathism , Retrognathia , Surgery, OralABSTRACT
PURPOSE: Kawasaki disease(KD) may be linked to primary infection by certain common viruses. KD concurrent with Epstein-Barr virus(EBV) suggests the possibility of an etiologic agent related to the KD rather than to the EBV infection itself, but, the influence on coronary complication is unclear. To establish whether infection with EBV contributed to the outcome of coronary artery lesions in patients with KD is the purpose of this study. METHODS: Retrospective studies were performed on 96 cases(age 2.48+/-1.94 years) of KD evaluated with serologic studies of EBV(EBV EA IgM, EBV EA IgG, EBNA IgG) at admission. We evaluated the clinical features and coronary outcome between a control group with KD and an EBV associated group with KD. RESULTS: On serologic studies of EBV, 62 patients(64.6%) with one more than positive results were in the EBV associated group with KD, in 30 patients with recent EBV infection, and in 32 patients with previous EBV infection. There were no significant differences between the control group and the EBV associated group in age, sex, and other clinical findings. Eighten patients (18.7%) had abnormal baseline echocardiogram, 13 patients(21.0%) among them were in the EBV associated group and 5 patients(14.7%) were in the control group; there was no significant difference between the two groups. Significantly 11 of 13 patients(84.6%) with coronary lesions had positive result of EBNA IgG, suggesting previous infection. There were no significant differences in retreatment and recurrence incidence. After retreatment, in 3 of 6 cases with the EBV associated group, and in no cases with the control group, cardiac complications were observed. CONCLUSION: Previous or chronic EBV infection may influence the occurrence of coronary lesion in patients with KD, even though patients with cardiac complication improved later.
Subject(s)
Humans , Coronary Vessels , Epstein-Barr Virus Infections , Herpesvirus 4, Human , Immunoglobulin G , Immunoglobulin M , Incidence , Mucocutaneous Lymph Node Syndrome , Recurrence , Retreatment , Retrospective StudiesABSTRACT
0.05).2. On horizontal section, The distance between the inferior alveolar canal and the lateral cortical plate of the mandibular ramus were 6.73+/-1.24mm minum, 7.70+/-1.44mm maximum.3. On coronal section, Outer mandibular angle were 4.84+/-2.37degrees right side, 4.93+/-2.12degrees left side.4. The design of the ideal true sagittal split ramus osteotomy is that posterior border of osteotomy must be limited vertically, at the right posterior point of lingula mandibularis and anterior border of osteotomy must be extended to mandibular body, anteroinferiorly.
Subject(s)
Adult , Humans , Osteotomy , Osteotomy, Sagittal Split Ramus , Recurrence , Temporomandibular Joint DisordersABSTRACT
The present study was performed to evaluate the comedogenicity of several cosmetic ingredients, vegetable oils, steroid and sulfur. The test materials were applied to one ear canal of rabbits for 6 weekdays for 2 weeks. The results were as follows: l. Among the 14 cosmetic ingredients, no comedogenicity was shown in propylene glycol and petrolatum, mild comedogencity in squalene, acetylated lanolin, polyethylene glycol, cetyl aIcohol and Tween 80. Propylene glycol, sodium lauryl sulfate and oleic acid showed moderate comedogenicity. Severe comedogenicity was observed in myristyl myristate, isopropyl myristate, butyl stearate and myristyl lactate. 2. Moderate to severe comedogenicity was shown in olive oil, sesame oil and corn oil and moderate comedogenicity in sulfur (8%) and betamethasone dipropionate (0.06%) R. Histopathologic grading were paralleled the findings of the naked eye.
Subject(s)
Rabbits , Betamethasone , Corn Oil , Ear Canal , Lactic Acid , Lanolin , Myristic Acid , Olea , Oleic Acid , Petrolatum , Plant Oils , Polyethylene Glycols , Polysorbates , Propylene Glycol , Sesame Oil , Sodium Dodecyl Sulfate , Squalene , Sulfur , Olive OilABSTRACT
Sensitivity to mercury appears to be not uncommon and perhaps the commonest contact with mercury in the general population is by amalgam dental restorations. Recently, we have experienced 2 cases of mercury dermatitis following amalgam dental restorations which were confirmed by the patch test. We report our cases with the patch test results and review the literatue on previous reports.
Subject(s)
Dermatitis , Patch TestsABSTRACT
We report 2 cases who have been diagnosed as lipodystrophia centrifugalis abdominalis juvenilis. The case 1 is l2 year-old girl. She had well-defined depression of skin on both inguinal area, lower portion of chest and both flanks which was noticed at the age of 7. The case 2 is 16 year-old boy with depressed lesions on Rt. inguinal area and abdomen which were noticed at the age of 6. Biopsy specimen showed absence of subcutaneous fat tissue in both cases.