ABSTRACT
Organic chemistry is an important foundation course for medicine, chemistry and other majors in universities and colleges. In this study, combined with the characteristics of pharmacy students in local colleges and universities, teachers introduced case teaching, enhanced curriculum connotation, and explored the integration of green chemistry concepts into the teaching process of organic chemistry, so as to cultivate pharmaceutical professionals with environmental protection concept and safety awareness. The practice shows that this teaching model not only improves students' attention to organic chemistry and learning efficiency, but also improves students' comprehensive quality.
ABSTRACT
Objective A quick and sensitive method was developed for determination of folic acid (FA) in infant formula milk powder. Method Perchloric aicd was added into infant formula milk powder to extract FA in ultrasonic wave condition. After solid-phase extraction (SPE) with C18 material as plug and reaction with potassium permanganate, FA was determined by reversed phase high-performance liquid chromatography (RP-HPLC). pH 5.0 and 0.1 mol/L sodium dihydrogen phosphate were used as mobile phase. the flow rate was 1 ml/min. BDS C18 was used as separating column and column temperature was 30 ℃. Results The linear range of the method was 0.005-1.5 ?g/ml. The average recovery was 91.7%- 98.3%. The relative standard deviation (RSD) was 2.5%. The limit of detection was 0.005 ?g/ml, and the limit of quantification was 0.017 ?g/ml. Conclusion The method has following advantages: simple, precise, accurate and easy to practice.