ABSTRACT
@#In this paper, cobalt chloride was used to stimulate human umbilical vein endothelial cells (HUVEC) to establish a model of abnormal hypoxic injury, to investigate the effect of heparin-derived oligosaccharides (HDO) on glycolysis in HUVEC cells and its molecular mechanism.The experiment was divided into the control group (FBS-free DMEM medium), the model group (FBS-free DMEM medium +50 μmol/L CoCl2), and the HDO group (modeling+0.01, 0.1, 1 μmol/L HDO).Firstly, a biochemical kit was used to detect the effects of HDO on glucose uptake and lactic acid accumulation in HUVEC cells, then Western blot and qPCR were used to detect the effects of HIF-1α, GLUT-1 and LDHA gene transcription and protein expression, and finally, PI3K/Akt signaling pathway was detected.The results showed that HDO inhibited glucose uptake and lactate production, down-regulated the expression of HIF-1α, GLUT-1, and LDHA, and affected the activation of the PI3K/Akt signaling pathway.HDO could regulate the glycolysis level of HUVEC cells by inhibiting the activation of the PI3K/Akt/HIF-1α signaling axis.
ABSTRACT
Objective@#To investigate the interest and awareness of traditional Chinese medicine undergraduates on the course of Chinese medical literature, and to provide suggestions for further enhancement of undergraduates' cognition and in-depth study of this subject.@*Methods@#Taking Shandong University of Traditional Chinese Medicine as an example, 726 undergraduates were sampled and randomly sampled by stratified sampling, and the interest and awareness of Chinese medical literature were suveyed and summarized, and the factors that led to this situation were analyzed.@*Results@#A total of 119 (16.4%) knew and took the course, 78 (10.7%) said they often read Chinese medical literature on their own initiative, 455 (62.7%) said that the factors affecting their access to Chinese medical literature were reading difficulties. 410 (56.5%) students suggested that the course of Chinese medical literature should be open as an optional course.@*Conclusions@#The undergraduates have high interest and low awareness of the Chinese medical literature. It is suggested that the school should carry out curriculum reform and organize relevant publicity activities in order to increase the awareness of the undergraduates on the Chinese medical literature.
ABSTRACT
Objective To investigate the interest and awareness of traditional Chinese medicine undergraduates on the course of Chinese medical literature, and to provide suggestions for further enhancement of undergraduates' cognition and in-depth study of this subject. Methods Taking Shandong University of Traditional Chinese Medicine as an example, 726 undergraduates were sampled and randomly sampled by stratified sampling, and the interest and awareness of Chinese medical literature were suveyed and summarized, and the factors that led to this situation were analyzed. Results A total of 119 (16.4%) knew and took the course, 78 (10.7%) said they often read Chinese medical literature on their own initiative, 455 (62.7%) said that the factors affecting their access to Chinese medical literature were reading difficulties. 410 (56.5%) students suggested that the course of Chinese medical literature should be open as an optional course. Conclusions The undergraduates have high interest and low awareness of the Chinese medical literature. It is suggested that the school should carry out curriculum reform and organize relevant publicity activities in order to increase the awareness of the undergraduates on the Chinese medical literature.