ABSTRACT
Objective To explore if strand breaks of DNA in human early chorionic villus cells in uterus were induced by diagnostic ultrasound and to evaluate the method used for detection of single-stranded breaks and doublestranded breaks in human DNA. Methods 60 normal pregnant women aged 20-30, who underwent artificial abortion during 6-8 weeks of gestation, were randomly divided into 2 experimental groups: All 30 cases were exposed to diagnostic ultrasound in uterus for 10 minutes, and 24 hours later chorionic villi were extracted; the other 30 cases were taken as the control group. Single-stranded DNA and double-stranded DNA in villus cells in all cases were isolated by the alkaline unwinding combined with hydroxylapatite chromatography, and were quantitatively detected using32 P-labeled Alu probe for dot-blotting hybridization. Results There was no significant difference in quantity and percentage in single-stranded DNA and double-stranded DNA between 2 groups (P＞0.05). 32 P-Alu probe could only hybridize with human DNA, and could detect DNA isolated from as few as 2.5 × 103 chorionic villus cells and 0.45 ng DNA in human leukocytes. Conclusion The results suggested that there were no DNA strand damages in human chorionic villus cells when the uterus was exposed to diagnostic ultrasound for 10 minutes. The method, 32P-Alu probe for dot-blotting hybridization, was even more specific, sensitive and accurate than conventional approaches.
ABSTRACT
285 cases of maternal deaths in Xian area from1980～1990 were reported. The average maternalmortality in urban and rural areas were 16. 3/100000 and 73. 8/100000 respectively, a differenceof 4. 5 times. The main cause of maternal deathwas obstetrical hemorrhage (49. 8%), the nextwere pregnacy indced hypertension and cardiac dis-eases. Of the 285 cases,36. 8% had no antenatalcare. In urban areas maternal death occarred all inhospitals, but in the rural areas 39. 2% happenedat home or on the way to hospitals. Positive. sug-gestions were given according to the major influ-ence factors on safe motherhood.
ABSTRACT
96 cases of maternal death occurred in our hospital from 1960～1989 were reported. The average maternal mortality rate was 170.7/100 000.54.2% of the total death were caused by direct obstetric events. The sequences of the main causes were: induced by pregnancy hypertention, liver disease,obstetric hemorrage,cardiac disease. The maternal care in these cases was unsatisfactory. The average number of antenatal examination was only 1.8. 40.6% died of delivering at home. Most of them were sent to our hospital in emergency. Positive suggestion were given according to the analytic result.