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To explore the antiviral activity of nano-realgar against herpes simplex virus Type II (HSV-2) in vitro. Methods: Acyclovir (ACV) as a positive control, the cytotoxicity of nano-realgar at different concentrations (including 200.00, 150.00, 100.00, 50.00, 25.00, 12.50, 6.25, 3.13, 1.54, 0.78, 0.39 and 0 mg/L) on normal Vero cells were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. HSV-2 virus titer was determined by plaque assay, and the Vero cells model of HSV-2 infection was established. Subsequently, the antiviral effects of nano-realgar at different concentrations (including 20.00, 10.00, 5.00, 2.50, 1.25, 0.63, 0.31, 0.15, 0.08, 0.04 and 0 mg/L) on infected cells model were evaluated by the observation of cytopathic effect (CPE) and MTT method under the 3 modes including pre-treatment, treatment and direct inactivation. Results: The 50% cytotoxic concentration (CC50) of nano-realgar on Vero cells was 37.15 mg/L. The titer of HSV-2 was 7.30 log PFUs/mL. In the 3 modes, the half-maximal effective concentration (EC50) of nano-realgar on HSV-2 infected Vero cells were 0.13, 1.80 and 0.52 mg/L, and the corresponding therapeutic index (TI) were 285.77, 20.64, 71.44, respectively. The TI value of nano-realgar on pre-treatment mode was higher than that of nano-realgar on treatment and direct inactivation modes. Conclusion: Nano-realgar can play a good anti-HSV-2 activity in the 3 modes (pre-treatment, treatment and direct inactivation), and the anti-HSV-2 efficacy of nano-realgar on pre-treatment mode is better than that of nano-realagr on other 2 modes.
Subject(s)
Animals , Antiviral Agents , Arsenicals , Chlorocebus aethiops , Herpesvirus 1, Human , Herpesvirus 2, Human , Sulfides , Vero CellsABSTRACT
Objective To observe the toxicity and effection of three dimensional conformal radiation therapy(3DCRT)based on conventional fractionation radiotherapy in combination with chemotherapy in non-small-cell lung cancer(NSCLC).Methods 62 NSCLC patients with stage Ⅲ were chosen as research objects.All the patients received chemotherapy two cycles with NP regimen firstly(NVB 40 mg d1,8,DDP 30 mg d2-5),then they accepted radiotherapy in twentith day after chemotherapy when hemocyte got normal.In the first half period of the radiotherapy,conventional radiotherapy were given with 2 Gy/f,after the dose got 40 Gy/20 f,then three dimensional conformal radiation therapy were given with 4～6 Gy/f every 1 to 2 days until the total dose got 24～30 Gy/4～6 f.The patients with supraclavicular lymphnode metastatic carcinoma received conventional fractionation radiotherapy with 60Co or 6 MV-X ray combined electronic ray until the dose(DT)up to 64～66 Gy.After radiotherapy,the patients accepted chemotherapy two cycles with NP regimen again.Results All the 62 patients completed treatment plan,and follow-up survey had lasted for 3 years.The short-term efficacy was evaluated in 3 months after radiotherapy. Among 62 patients, 9 cases gained a complete remission(CR),40 cases partial remission(PR),and the total remission was 79.0%.The acute toxicity showed acute radiation esophagitis,radiation pneumonia and acute gastrointestinal reaction.However,expectant treatment was effective.The 1,2,3 year survival rate was 7 1.0%,48.4%,30.6%,respectively.The 1,2,3 year local control rate was 80.6%,62.9%,40.3%,respectively.Conclusion 3DCRT based on conventional fractionation radiotherapy for NSCLC have good effection and mild toxicity.
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[Objective] To observe the therapeutic effect of heat-clearing and diuresis-promoting herbal medicine for hyperuricemia induced by pyrazinamide. [Methods] Eighty-six patients with pyrazinamide-induced hyperuricemia were randomized into 2 groups: group A ( n = 43) received antiphthisic drugs (including isoniazid, rifampin, pyrazinamide and ethambutol) , allopurinol tablets and Modified Simiao Powder (one dose per day); group B ( n = 43) was treated with antiphthisic drugs and allopurinol tablets. Fourteen days constituted one treatment course and the two groups were treated for 2 courses. After treatment, the therapeutic effect was assessed and the changes of blood uric acid level were observed. [Results] In group A, 28 (65.12%) were cured, 12 (27.91%) effective, 3 (6.97%) ineffective and the total effective rate was 93.02%; in group B, 16 (37.21%) were cured, 17 (39.53%) effective, 10 (23.26%) ineffective and the total effective rate was 76.74% . The therapeutic effect in group A was better than that in group B (P
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Objective:To develop a method for the determination of taurine in Taurine Soft Capsules by RP HPLC.Methods: Taurine was derivated by phthalaldehyde and ethanethiol. The analytical column was C 18 . The UV detective wavelength was set at 360nm. The mobile phase was methanol water phosphate buffer (1∶1∶1). The external standard method was used. Results: The recovery was 95.8%～107.2%. RSD was 2.1%. The linear range of concentration was 6.7 ～40.4 mg/mL. The minimum detectability was 2.47mg/mL. Conclusions: The method is simple, quick and accurate.