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Objective To investigate the expressions of CYCLIND1 CDK4 in pancreatic cancer and their relationship with clinlicopathological parameters.Methods Immunohistochemical detector with new second generational tow steps method was used to detect the expressions of CYCLIND1 CDK4 and PCNA in 48 cases of pancreatic cancer and 14 cases of chronic pancreatitis.Results The expressions of CYCLIND1 CDK4 proteins in pancreatic cancer were 70.85%(34/48)and 62.50%(30/48) respectively.The CYCLIND1 and CDK4 proteins were increased(P
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Aim To observe the adhesive process of the human gastric cancer cell line MKN1,and study the expression of integrin ?1;to investigate the mechanism of the inhibition of the adhesion process of MKN1 cells by destran sulfate(DS).Methods The MKN1 cells were cultured with DS or PBS,then stained with immunofluorescent cytochemistry and observed in fixed or living conditions with confocal laser scanning microscope.RT-PCR was used to analyze the cDNA expression of MKN1 cells.Results MKN1 cells adhered to culture dishes by the process of forming filopodia,changed into a flat shape,and then adhered to other cells to form a cell-monolayer.Integrin ?1 was intensively expressed in the cell membrane,where integrin ?1 formed clusters.DS inhibted the expression of integrin ?1 in cell membrane,and decreased the area of integrin ?1 clusters.DS-treated cells also tended to maintain a round shape by contracting the filopodia.In DS-containing culture dishes,some cells kept floating 4 hours after seeding.DS decreased the level of the cDNA expression of the adhered cells to 74% and of the floating cells to 38% of that of the cells in un-treated group,respectively.Conclusion The inhibition of the adhesion of MKN1 cells by DS was related to the suppression of the expression of integrin ?1.
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Objective:To investigate the mechanism for intraperitoneal injection of sapylin to enhance the anti-tumoral immune functions of peritoneal macrophages.Methods:72 cases of patients with early or middle stage gastrointestinal cancer were peritoneally injected saline or sapylin, 48 h or 24 h before operation. The peritoneal macrophages(PM?) was harvested during their operations. The number of the PM? was counted. The phagocytosis, the enzyme activity of LDH and ACP,and the NO secretion were analyzed. Using human gastric cancer cell line MKN1 as target cells, the anti-tumoral cytotoxicity of the PM? was observed.The greater omentum was harvested,both the number and the size of the omental milky spots were measured.Results:I.p. injection of sapylin significantly increased the number, the phagocytosis, the enzyme activities,NO secretion and anti-tumoral cytotoxicity of the PM?, as well as the number and the size of omental milky spots.Conclusion:By activating omental milky spots, intraperitoneal injection of sapylin increased the number and enhanced the immune function of PM?, the activated PM? showed enhancement of anti-tumoral cytotoxicity.
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ObjectiveTo assess activated carbon in the standard lymphadenectomy of gastric cancer.Methods183 cases undergoing radical gastrectomy before 1997 served as control group,87 consecutive cases after 1997 received intumour injection of activated carbon(AC) before undergoing gastrectomy.The areas and number of dissected lymph nodes,operative complication and hospital stay in subtotal gastrectomy and total gastrectomy were analyzed respectively.ResultsIn subtotal gastrectomy,the ratio of D 1 ,D 2 ,D 3 and D 3 +PAL over the total cases was 4 7%,10 3%,14 9% and 16 1% respectively in AC group and 25 7%,21 3%,9 9% and 2 7% respectively in control group.In total gastrectomy,the ratio of D 1 ,D 2 ,D 3 and D 3 +PAL was 3 4%,5 7%,21 9% and 23% respectively in AC group and 15 8%,14 8%,8 7% and 1 1% respectively in control group.The differences of the four procedures were all statistically significant between the two groups.The number of dissected lymph nodes in each case in AC group was more than that of control group.ConclusionsLocal injection of activated carbon is a simple,easy and safe method to increase the dissection of lymph nodes in radical gastrectomy.