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OBJECTIVE To clarify the influence factors of hearing impairment in mice caused by lack of folic acid and the mechanism.METHODS Mice were randomly divided into 2 groups,fed with no folic acid diet and normal diet for 10 weeks.Hearing was tested by ABR.Serum homocysteine,folic acid were detected.Immune imprint method was used to analyze the expression of related protein.Cochlear tissue was tested by histological method to observe morphological changes.At the same time using the TUNEL method was used to analyse the cochlear tissue cell apoptosis.RESULTS Analysis showed that serum folate levels in mice fed without folic acid diet dropped than that of the normal group,while homocysteine levels elevated.Through the brain stem auditory records and cochlear cell apoptosis of mice severe hearing loss was found in folate deficiency group.Western blot detection showed enzyme catalysis homocysteine products increased by 50％ in folate deficiency group than that of the normal diet group.CONCLUSION The high homocysteine levels,and folate deficiency can cause hearing loss in mice and are related with the inner ear homocysteine metabolic disorders.
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BACKGROUND: Concentrated growth factor (CGF) fibrin membrane is involved in metabolism regulation, which has been applied in bone remodeling. Previous Studies have found that the cytoplasts of osteoblasts, interstitial cells and osteocytes in distraction osteogenesis are positive for osteoprotegerin and receptor activator nuclear factor kappa B ligand (RANKL).OBJECTIVE: To explore the mechanism of bone orthopedic effect and the promotion effect of CGF fibrin membrane on the distraction osteogenesis of the rabbit mandible.METHODS: Twenty-four white rabbits were randomly selected. Distraction osteogenesis was performed unilaterally in the rabbit left mandible (control group); CGF fibrin membrane was fixed on the inner surface of distractor, and the distraction gap was then completely wrapped prior to distraction osteogenesis in the right mandible (experimental group).At 1, 7, 14 and 28 days of consolidation period, the rabbits were sacrificed and the bilateral mandibles were taken for hematoxylin-eosin staining, western blot assay and immunohistochemical staining to detect the expression of RANKL and osteoprotegerin in the newborn bones; the osteogenesis in the distraction gap was observed and compared between two groups.RESULTS AND CONCLUSION: The osteoprotegerin positive cells and positive area percentage in the experimental group were significantly higher than those in the control group at 1, 7 and 14 days after distraction osteogenesis (P < 0.01). The RANKL positive rate and positive area percentage in the experimental group were significantly higher than those in the control group at 1 and 14 days after distraction osteogenesis (P < 0.05 or P < 0.01). Western blot assay showed a higher ratio of RANKL/osteoprotegerin in the control group than the experimental group (P < 0.01). Our findings indicate that CGF fibrin membrane can promote osteogenesis and mineralization in the distraction gap of the rabbit mandible, which is beneficial for distraction osteogenesis in the mandible.
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BACKGROUND:At present, there are no studies of comparing the effect of silk fibroins from different sources in repair of osteochondral defects. OBJECTIVE:To compare the effect of mulberry silk- and tussah-derived silk fibroin scaffold materials in repair of osteochondral defect. METHODS:Totaly 20 New Zealand white rabbits were obtained to prepare osteochondral defect models on the unilateral knee joint and randomly divided into five groups: control group, experimental group 1, experimental group 2, experimental group 3 and experimental group 4. Rabbits in the control group were not implanted any materials. In the experimental group 1, 3 layers of mulberry silk protein scaffolds stuck together to fil in defects. In the experimental group 2, one mulberry silk protein scaffold coated with transforming growth factor-β3 was stuck with two mulberry silk protein scaffolds coated with bone morphogenetic protein-2 to fil in defects. In the experimental group 3, three layers of tussah protein scaffolds stuck together to fil in defects. In the experimental group 4, one tussah protein scaffold coated with transforming growth factor-β3 stuck together with two tussah protein scaffolds coated with bone morphogenetic protein-2 to fil in defects. At 8 weeks post surgery, articular cartilage repair area was observed histopathologicaly. Type I and II colagen expressions were determined. RESULTS AND CONCLUSION:The colagen fibers in experimental group 1 were widely distributed in the ful-thickness defect area. The colagen fibers in the experimental group 2 were paralely distributed on the surface of repair area, verticaly distributed from the middle and bottom to the top direction. Colagen was observed on the surface of repair area in the experimental group 3. The cartilage-like cels presented clumped distribution on the surface and at the bottom of scaffold. The type I colagen expression in the repair area was strongly positive in these four experimental groups. The type II colagen expression in the repair area of experimental 1 and experimental 2 groups was weak. The type II colagen expression in the repair area of experimental 3 and experimental 4 groups was strongly positive. These results demonstrate that these two kinds of silk fibroins can both repair osteochondral defects, in which mulberry silk proteins tend to form bone tissue, and tussah silk proteins tend to form cartilage tissue.
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<p><b>BACKGROUND</b>The prevalence of metabolic syndrome (MetS) increased recently and there was still not a screening index to predict MetS. The aim of this study was to estimate whether brachial-ankle pulse wave velocity (baPWV), a novel marker for systemic arterial stiffness, could predict MetS in Chinese community population.</p><p><b>METHODS</b>A total of 2 191 participants were recruited and underwent medical examination including 1 455 men and 756 women from June 2011 to January 2012. MetS was diagnosed according to the criteria of the International Diabetes Federation (IDF). Multiple Logistic regressions were conducted to explore the risk factors of MetS. Receiver operating characteristic (ROC) curve was performed to estimate the ideal diagnostic cutoff point of baPWV to predict MetS.</p><p><b>RESULTS</b>The mean age was (45.35±8.27) years old. In multiple Logistic regression analysis, the gender, baPWV and smoking status were risk factors to MetS after adjusting age, gender, baPWV, walk time and sleeping time. The prevalence of MetS was 17.48% in 30-year age population in Shanghai. There were significant differences (χ(2) = 96.46, P < 0.05) between male and female participants on MetS prevalence. According to the ROC analyses, the ideal cutoff point of baPWV was 1 358.50 cm/s (AUC = 60.20%) to predict MetS among male group and 1 350.00 cm/s (AUC = 70.90%) among female group.</p><p><b>CONCLUSION</b>BaPWV may be considered as a screening marker to predict MetS in community Chinese population and the diagnostic value of 1 350.00 cm/s was more significant for the female group.</p>
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Adult , Female , Humans , Male , Middle Aged , Ankle Brachial Index , Methods , Logistic Models , Metabolic Syndrome , Diagnosis , ROC CurveABSTRACT
BACKGROUND: Wnt signaling pathway is a key regulator of cellular proliferation and differentiation, but its correlation with neural differentiation of bone marrow mesenchymal stem cells (BMSCs) is not very clear. OBJECTIVE: To find out the molecules of the Wnt family which are involved in differentiation of rat BMSCs into neuron-like cells. METHODS: The rat BMSCs were separated and cultured in vitro. The morphology of the BMSCs was observed. Flow cytometry analysis was performed to detect cell phenotype CD44, CD9, CD34 and CD45. Wnt3a and Wnt5a were respectively combined with basic fibroblast growth factor to induce BMSCs differentiation into neuron-like cells, and then were identified by using immunocytochemistry and RT-PCR. RESULTS AND CONCLUSION: The BMSCs were long-spindle. CD9 and CD44 were highly expressed, while CD34 and CD45 were lowly expressed. Nestin and neuron specific enolase were positive but glial fibrillary acidic protein were not obviously expressed when they were cultured with Wnt3a. In Wnt5a group, Nestin expression was weakly positive, while neuron specific enolase and glial fibrillary acidic protein were negative. RT-PCR result revealed Nestin expressed both before and after induction in the Wnt3a induced group, neuron-specific enolase exhibited apparent amplified bands 5 days after the induction, and more apparent at 10 days. A weak amplification band of glial fibrillary acidic protein could be seen at 10 days after the induction. In Wnt5a and control groups, BMSCs induced by 10 days weakly expressed Nestin, while neuron-specific enolase and glial fibrillary acidic protein were almost not expressed. It is indicated that Wnt3a molecule can promote the differentiation of BMSCs cultured in vitro to neuron-like cells.
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A medical metrology supervision system is proposed.It analyses the composition structure including data input and print,statistic inquiry,data acception and report as well as data file management.The technological characteristics are analyzed including system classification,authorization and password control,system safety and file output.Making the military medical metrology supervision more standardized and scientific,the system remarkably improves the work efficiency.
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Objective To study the spare demand rate and storage quantity probability model for military medical equipment in order to make a valuable reference to the decision-making of medical organization for the further medical support.Methods The reliability and maintainability of spare in the process of equipment maintenance were studied,and the signifi-cant factors which have influence on spare demand rate were analyzed.By using Passion theory,spare demand rate proba-bility model of medical equipment was deduced,especially for repairable components and un-repairable components spare.By calculating the probability of replaceable spare,a useful method to scientifically make out the storage quantity of mili-tary medical equipment was provided.Results The models of spare demand rate and storage quantity were made for mili-tary medical equipment maintenance.Conclusion The solution of the model is simple and convenient and the calculating results shows that the spare demand rate and storage quantity is close to the reality.It can meet the requirements of pre-diction of spare demand rate and determination of the storage quantity in maintenance support of medical equipment in peacetime.
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We used epidemiological method to analyse vegetable salmonella carrier rates in six differ- ent vegetables at 12 vegetable markets in two urban districts of Kunming city from March to May 1992. Results showed the total vegetable salmonella carrier rate was 22.71%. showed no significant difference. The carrying rate of cordate houtlugnia is the highest in the six kinds of vegetables. There was a rising tendency of salmonella carrier rate in vegetables from March to May. Two hundred and seventy salmonella strains were isolated, which belonged to six groups of A, B, C, D, E, F, and 14 serum types. Among them, paramount S. Paratyhi-A bacterium took predominance and accounted for 48.89%, the next were S. tyhi and S. Paratyhi-B.