ABSTRACT
Objective To explore the consistency and significance of optical coherence tomography (OCT) and clinical and histopathological findings in adoptively transferred uveitis in mice.Methods The adoptively transferred experimental autoimmune uveitis (EAU) model was established by intraperitoneal injection of antigen-specific T cells in C57BL/6 mice.Since 9 days after transferred,inflammation of eyes was observed by indirect ophthalmoscope with +90D lens and record clinical scores every 3 days.The disease was divided into 6 phases including onset phase,early phase,pre-peak phase,peak phase,resolution phase and late phase of EAU,which respectively corresponding to clinical score 0.5,1.0,1.5-2.0,2.5-3.0,1.0-2.0 and less than 1.0.Since 9 days after transferred,the retina and retinal thickness (RT) was measured by spectralis OCT about 1 disc from the disc edge in 10 time points including 9,11,16,21,25,30,35,40,50 and 60 days after transferred.The OCT score was recorded as from 0.0 to 4.0.After transferred 9,21 and 60 days,the mice were killed and eye balls were examined in histology.OCT score,clinical score and histology in the mouse were compared and analyzed.Results The disease was divided into onset phase,early phase,pre-peak phase and peak phase of EAU,which respectively corresponding to 9,16,21 and 26 days after transferred.In four phases,OCT score were 0.5,1.0,2.0 and 4.0 respectively.After transferred 30 days,which was in resolution phase of EAU,the inflammation cells in vitreous were decreased and OCT score was 3.0.After transferred 60 days,which was in late phase of EAU,inflammation cells in vitreous were disappeared and retina was atrophic topically.The histology showed the vitreous has slight inflammation cells and retinal structure was normal at onset of EAU.The vitreous has massive inflammation cells and retina structure was disorder at pre-peak of EAU.And in resolution phase of EAU,the inflammation cells in vitreous were slightly and retina was atrophic and thinned.The data in this study demonstrated that OCT score was well correlated with clinical score in EAU (r=0.957 9,P＜0.000 1).Conclusion OCT and clinical and histopathological findings in adoptively transferred uveitis in mice were consistency and OCT is contribute to evaluate the disease dynamically and quantifiably.