ABSTRACT
<p><b>OBJECTIVE</b>To study the correlation of Pinellia ternata agglutinin (PTA) and toxicity of P. ternata raphides and to find out the toxic mechanism of P. ternata.</p><p><b>METHOD</b>PTA has obvious effect of pro-inflammation. The model of rats peritonitis was used to study the dose-toxicity and time-toxicity relationship of the effect by detecting the releases of inflammatory mediators PGE2 in the exudates. The model of Draize rabbit eye test was applied to determine the correlation of PTA and toxicity of raphides by pathological examination.</p><p><b>RESULT</b>PTA enhanced the content of PGE2 and protein in rats peritoneal cavities concentration dependently. With PTA concentration increased, PTA enhanced the inflammation induced by raphides to rabbit eyes, but PTA alone had no toxicity response.</p><p><b>CONCLUSION</b>PTA had obvious effect of pro-inflammation. The toxic mechanism of P. ternata was PTA induced inflammation only when the raphides pierce into the organization.</p>
Subject(s)
Animals , Male , Rabbits , Rats , Agglutinins , Chemistry , Toxicity , Eye , Inflammation , Pinellia , Chemistry , Rats, Sprague-DawleyABSTRACT
<p><b>OBJECTIVE</b>To explore new toxicity-reducing methods of Pinellia Rhizoma prepared by ethanol and the latest technical parameters.</p><p><b>METHOD</b>Pinellia Rhizoma is prepared with ethanol. The orthogonal experimental design was adopted for investigating amount of ethanol, preparing time, ethanol concentration and preparing temperature. The optimal technology was determined by the comprehensive score of toxicological indicators of PGE2 content of rat celiac percolate, with the rabbit conjunctival irritation test as the intuitive validation on toxicology reduction. The pharmacodynamics validation was used to determine the reasonability of the preparation process.</p><p><b>RESULT</b>The optimal technology was that Pinellia Rhizoma was prepared by 75% ethanol at the temperature of 60 degrees C by 4 days, and then dried. The effect of relieving cough, reducing sputum and anti-inflammatory of Pinellia Rhizoma is not reduced after prepared by ethanol.</p><p><b>CONCLUSION</b>The optimal technology of Pinellia Rhizoma prepared by ethanol is simple and reasonable that it can be used as the new method to reduce toxicity and keep efficacy of Pinellia Rhizoma.</p>
Subject(s)
Animals , Female , Male , Mice , Rabbits , Rats , Anti-Inflammatory Agents , Chemistry , Pharmacology , Cough , Drug Therapy , Desiccation , Drugs, Chinese Herbal , Chemistry , Pharmacology , Ethanol , Chemistry , Hot Temperature , Mice, Inbred ICR , Pinellia , Chemistry , Toxicity , Plants, Medicinal , Chemistry , Toxicity , Rats, Sprague-Dawley , Rhizome , Chemistry , Toxicity , Sputum , Technology, Pharmaceutical , Methods , Time Factors , Toxicity TestsABSTRACT
<p><b>OBJECTIVE</b>To study and compare the changes of toxicity of Euphorbia pekinensis, E. kansui and E. ebracteolata before and after being prepared by vinegar.</p><p><b>METHOD</b>Small intestinal accentuation of mice and peritoneal macrophage NO release experiments were assessed to investigate the changes of toxicity of the three Chinese Medicines of Euphorbia before and after being prepared.</p><p><b>RESULT</b>E. pekinensis, E. kansui and E. ebracteolata and vinegar can obviously promot small intestinal accentuation and peritoneal macrophage NO release with the intensity of toxicity in the order of E. kansui > E. pekinensis > E. ebracteolata. After being prepared with vinegar, the toxicity of the three medicines decreased obviously compared to crude one.</p><p><b>CONCLUSION</b>E. pekinensis, E. kansui and E. ebracteolata can induce inflammation and accelerate enterokinesis. After being prepared with vinegar, the irritation on Euphorbia decreased obviously.</p>