ABSTRACT
Objective:To explore and evaluate the effect of clinical toxicity nursing on the therapeutic effect of paclitaxel combined with cisplatin in the treatment of recurrent cervical cancer.Methods:Sixty patients with recurrent cervical cancer treated in the Second Hospital of Tianjin Medical University from May 2017 to May 2019 were randomly divided into two groups. The control group was given paclitaxel chemotherapy and routine nursing, and the study group was given paclitaxel combined with cisplatin and clinical toxicity nursing.Results:In the study group, the total effective rate of 93.3% was significantly higher than that of 73.3% in the control group ( P<0.05), and the levels of inflammatory indexes, including C-reactive protein (CRP), interleukin-6 (IL-6), white blood cell (WBC) and erythrocyte sedimentation rate (ESR) in the study group were lower than those in the control group ( P<0.05). The incidence of toxicity in the study group was 10.0% lower than that in the control group(36.7%, P<0.05). Conclusions:The use of paclitaxel combined with cisplatin chemotherapy for recurrent cervical cancer and clinical toxic reaction nursing can improve the effectiveness rate of treatment, reduce the levels of various inflammatory indicators, and reduce the incidence of toxic reactions with high safety, thus prolonging the survival time of patients, making it worthy of clinical application.
ABSTRACT
Objective To explore the effects of C/EBPα knockout in podocyte on diabetic nephropathy and its mechanisms.Methods C/EBPαloxp/loxp mice were crossed with podocin-cre mice to obtain F1 hybrids and then propagated until homozygous mice (C/EBPαf/f) were obtained.Diabetic nephropathy (DN) models were established by low-dose streptozotocin (STZ,100 mg/kg) administration after 25 weeks of normal diet or 45％ high-fat diet treatment,and biochemical indicators of blood and urea were measured.The morphological characteristics and the proteins regulating oxidative stress and mitochondrial function were detected.Results The type 2 DN models were successfully constructed based on transgenic mice.The kidneys of 8-month-old C/EBPαf/f mice did not show obvious morphological changes,but after constructing DN models,they showed obvious renal impairment,inflammation and oxidative stress.Compared with wild-type DN mice,the protein levels of nephrin and E-cadherin in DN C/EBPαf/f mice with DN were significantly decreased (P ＜ 0.01);fibronectin and Nrf2 protein levels were all increased (all P ＜ 0.05).Keap1,phospho-AMPK and mitochondrial function related genes Pgc-1α protein levels were all decreased (all P ＜ 0.05).Conclusion Podocyte C/EBPα knockout exacerbates diabetic nephropathy by promoting fibrosis and inhibiting Pgc-1α-mediated mitochondrial antioxidant function.