ABSTRACT
Objective To elucidate the role of the glutathione(GSH)/glutathione peroxidase 4(GPx4)-mediated ferroptosis pathway in preventing age-related hepatocyte peroxidation injury following aerobic exercise in mice,and to provide a new target for improving liver aging and metabolism disorders.Methods Twenty specific-pathogen-free C57BL/6 male mice aged 52 weeks were divided randomly into an elderly control group(EC group)and elderly exercise group(EE group)(n = 10 per group).The mice performed moderate-intensity exercise with incremental loads(1～2 weeks 14 m/min,3～4 weeks 15 m/min,5～10 weeks 16 m/min,11～16 weeks 17 m/min,60 min/day,slope 0°)for 16 weeks.After perfusion of the ascending aorta,the lateral liver lobes were harvested and sectioned for hematoxylin and eosin staining and ultrathin sections were used for transmission electron microscopy.Levels of 8-hydroxy-2 deoxyguanosine(8-OHdG)and 4-hydroxynonenal(4-HNE)in the liver and serum interleukin-6(IL-6)were detected by enzyme-linked immunosorbent assay.Hepatic glycogen,triglycerides(TG),malondialdehyde(MDA),nicotinamide adenine dinucleotide phosphate(NADPH),and GSH were determined by colorimetry.Hepatic GPx4,glucose transporter(GLUT2),NAD(P)H:quinone oxidoreductase 1(NQO1),and solute carrier protein 7 family member 11(SLC7A11)were detected by Western Blot.Results(1)Oxidative damage to hepatocytes was effectively delayed,normal mitochondrial structure and glycogen storage in hepatocytes were maintained.(2)Hepatic GSH and NADPH contents were significantly increased in EE mice compared with EC mice(P<0.01).(3)In addition,liver levels of 8-OHdG,4-HNE,MDA,and non-heme iron were significantly decreased in the EE group compared with the EC group(P<0.01).(4)Expression levels of GPx4,NQO1,and SLC7A11 in the liver were increased(P<0.01)while NOX2 expression was decreased(P<0.01)in the EE group compared with the EC group.Conclusions GSH synthesis was increased in aged mice following aerobic exercise,providing reaction substrates for GPx4 and activating the GSH/GPx4 pathway.Ferroptosis was inhibited,thus improving hepatocyte peroxidation damage caused by aging,and maintaining the normal structure and physiological function of hepatocytes.
ABSTRACT
Objective To explore the clinical value of MCV combined with Hb electrophoresis in detection of tha-lassemia. Methods Selected 150 cases of patients with thalassemia as the observation group, and other 150 cases of non-thalassemia patients were selected in our hospital as control group. MCV and Hb electrophoresis examination were conducted in two groups of patients, and the positive rates were recorded. And then we analyzed the specificity and sensitivity of the two methods as well as the combined examination. Results In the single MCV or Hb electrophoresis detection, the positive rate in observation group patients was higher than this in the control group, and the differences were statistically significant (P<0.05). The sensitivity and specificity of MCV detection were separately 91.33% and 76.67%, and the sensitivity and specificity of Hb electrophoresis were separately 85.33% and 79.67%. And when we conducted the combined detection, the sensitivity decreased, but the specificity increased. Conclusion For the patients with thalassemia, by using the method of MCV combined with Hb electrophoresis, we can improve the specificity in de-tection of disease. It is worthy of promotion in clinical use.