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Objective To explore the clinical application of antibiotics Ceftazidime(CAZ) and Cefotetan(CTT)by analysis susceptibility and scatter of the CAZ adn CTT against Escherichia coli(ECO) and Klebsiella pneumoniae(KPN).Methods The drug sensitivity analysis of 1 311 strains of ECO and 898 strains of KPN isolated from 2012 to 2015 and the relationship between CAZ and CTT was analyzed by using the Whonet 5.6 software.Results The resistance rate of ESBL+ KPN to CAZ was 41.2％ and the rate to CTT was 14.1％,the difference was statistically significant(P<0.05).The resistance rate of ESBL+ ECO to CAZ was 34.6％ and the rate to CTT was 1.1％,the difference was statistically significant(P<0.05).The average value of MIC of CAZ was highest in group of ESBL+KPN,it was 6.39 μg/mL.And it was lowest in group of ESBL-KPN,it was 1.37 μg/mL.The average value of MIC of CTT was highest in group of ESBL+KPN,it was 6.8 μg/mL.And the lowest was in group of ESBL-KPN.The range of MIC of CAZ was 1-64 μg/mL,and the range of CTT was 4-64 μg/mL in all groups.The cross sensitivity of CAZ and CTT was more than 90.0％.The cross resistance was less than 5.0％.The cross sensitivity of CAZ and CTT was less than 70.0％ in ESBL+ group.And the cross resistance was up to 13.4％.Conclusion The cross resistance and cross sensitivity of the two antibiotics is very important in guiding clinical antibiotic selection or replacement.
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<p><b>OBJECTIVE</b>To investigate the expression of BATF, a member of the activator protein-1 family, in the renal tissues of mice with lupus nephritis.</p><p><b>METHODS</b>The renal tissues from 24-week-old female MRL/lpr mice and age- and sex-matched C57BL/6 mice were examined for BATF protein expressions using Western blotting and for expressions of BATF, RORγt and IL-17A mRNA using quantitative real-time PCR. The results of laboratory examinations were analyzed in relation with the histopathology of the mice.</p><p><b>RESULTS</b>The urinary protein and ds-DNA levels were significantly higher in MRL/lpr mice than in the control mice (P<0.05). Compared to normal control mice, MRL/lpr mice showed obvious glomerular fibrosis and inflammatory cell infiltrating with significantly increased BATF protein and mRNA expressions (P<0.05) and RORγt and IL-17 mRNA expressions in the renal tissues (P<0.05). In MRL/lpr mice, the expression of BATF mRNA was positively correlated with the RORγt and IL-17A mRNA expressions in the renal tissues.</p><p><b>CONCLUSION</b>The renal expressions of BATF protein and mRNA is increased in MRL/lpr mice. BATF may participate in the immunopathogenesis of lupus nephritis by enhancing Thl7 cell response.</p>
Subject(s)
Animals , Female , Mice , Basic-Leucine Zipper Transcription Factors , Metabolism , Blotting, Western , Interleukin-17 , Metabolism , Kidney , Metabolism , Kidney Glomerulus , Pathology , Lupus Nephritis , Metabolism , Mice, Inbred C57BL , Mice, Inbred MRL lpr , Nuclear Receptor Subfamily 1, Group F, Member 3 , Metabolism , RNA, Messenger , Real-Time Polymerase Chain ReactionABSTRACT
Objective To study the limitation analysis of Staphylococcus spp Susceptibility Test Kit ATB STAPH 5 from Bi-oMerieux in clinical application and to propose remedy.Methods The coverage area and break point of antibiotics ATB STAPH 5 from BioMerieux were analysed by using CLSI M100-S23 as standard.Results The kit ATB STAPH 5 did not include chloromycetin,oxazolidinones and lipopeptide three antibiotics that were recommend by CLSI.The 16 kinds of antibi-otics break point in the kit ATB STAPH 5 were all not inconsistent or did not contain with the standard of CLSI M100-S23. It may lead to error drug sensitivity tests,false sensitive or false drug resistance.Conclusion Only using this kit ATB STAPH 5 for Staphylococcus spp ,it may occurre errors or mistakes.Other experiments must be corrected before the re-port.
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Objective To compare the value of time-resolved fluorescence immunoassay (TRFIA) and ELISA for the detection of HBV serological markers .Methods To detect the HBV serological markers in 359 samples with low concentrations of HBsAg by TRFIA and ELISA respectively ,and to compare the results of the two methods .Results The levels of HBV serological markers detected by TRFIA were higher than those detected by ELISA .The positive rates of HBsAg ,HBeAg and HBcAb detected by TR-FIA and ELISA were significantly different(P0 .05) .Conclusion Com-pared with ELISA ,TRFIA has higher sensitivity in the detection of low HBsAg concentration samples ,and it is valuable to be ap-plicated in clinical .