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Objective To investigate the effects of propofol on death-associated protein kinase (DAPK) mRNA expression in a rat model of traumatic brain injury (TBI). MethodsSixty male Wistar rats with a mean age of 3-4 months and mean body weight of 250-300 g were randomly divided into 6 groups ( n = 10 each) : Ⅰ normal control;Ⅱ sham operation; Ⅲ TBI; Ⅳ normal saline (NS) +TBI;Ⅴ fat emulsinn+ TBI and Ⅵ propefol + TBI. TBI was produced according to Feeney et al. In group Ⅲ-Ⅵ the animals were killed and their brains were removed at 24 h after brain contusion. NS 2 ml·kg-1 · h-1 , 10% fat emulsion 2 ml · kg-1 · h-1 and propofol 2 ml·kg-1·h-1 were infused iv for 4 h after lead trauma in group Ⅳ, Ⅴ and Ⅵ respectively. DAPK mRNA expression in the brain tissue was determined by RT-PCR and neuronal apeptosis was assessed by TUNEL. Results The expression of DAPK mRNA and neuronal apoptosis were significantly increased in the TBI group as compared with normal control and sham operation groups. Propefol infusion significantly attenuated neuronal apoptosis and reduced DAPK mRNA expression as compared with TBI, NS and FE groups. ConclusionPropofol can protect the brain from traumatic injury by suppression of the expression of DAPK mRNA.
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Objective To study the effect of methylprednisolone therapy assisted by direct current field to treat spinal rord injury.Methods In order to make up for the defect that the direct current field could tiol reduce or remove spinal-cord edema,methylprednisolone was used 2 hours after spinal-cord injury.24 Chinese dogs wore randomly divided into two groups.Their spinal cords were made completely injured with Allen method.In Group A the electric stimulator was put into the body directly 6 hours after the spinal cord injury,while in Group B a large dose of rneuiylprednisolone was first injected 2 hours after thf spinal cord injury and then the electric stimulator was put into the body 6 hours after the spinal cord injury.In both groups the nerve function,cortical somatosensory evoked potential and three kinds uf morphometric changes were observed at 1,2,and 3 months after the injury.Results All the indexes mentioned above in Group B were much better than those in Group A.The differences between the two groups were statistically significant.Conclusion The direct current field can promote spinul curd regeneration effectively.A large dose of methylprednisolone assisted by direct current field has synergislic effects in treatment of spinal cord injury,especially in accelerating neurological functional recovery.
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Based on our clinic experience in the diagnosis and treatment of acute pancreatitis,this article discussed some basic problems of the chinese guide of acute pancreatitis management,such as the definition of acute pancreatitis,classification of acute pancreatitis,diagnosis and treatment of acute pancreatitis.
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Objective To study the relation between p38 MAPK pathway and multidrug resistance of stomach cancer. Methods The activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR was examined by immunoprecipitation after treating with vincristine for 0,2,15,30,60 minutes. Results Vincristine could activate the activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR. The difference between them was that in cell SGC7901 vincristine activate the p38 MAPK rapidly whereas in cell SGC7901/VCR slowly. Conclusion Vincristine could affect the activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR.
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To investigatethe relationship between p38 MAPK pathway and multidrug resistance of stomach cancer. The activity of p38 MAP kinase in stomach cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR were examined by immunoprecipition after adriamycin treatment SGC7901 for 0, 2, 15, 30 and SGC7901/VCR for 0, 2, 15, 30, 60 minutes,respectively. The results indicated that adriamycin could activate the activity of p38 MAP kinase in gastric cancer cell SGC7901 with time dependency, but could decrease remarkably the activity of p38 MAP kinase in gastric cancer multidrug resistant cell SGC7901/VCR after adriamycin treatment it for 15 minutes. It suggested that adriamycin could affect the activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR.