ABSTRACT
Objective To explore the influencing factors of sub-health status among the migrant workers in Dongguan City.Methods A total of 740 migrant workers in Dongguan city were extracted by the stratified random sampling method.The SubHealth Measurement Scale Version 1.0(SHMS V 1.0) was adopted to test the health status.The data were analyzed by Logistic regression analysis.Results The univariate analysis showed that the marital status,average daily working time,monthly family per capita income,living conditions,drinking,breakfast,nutritional status,vigils,living conditions satisfaction,sedentary desk operation and experiencing negative events had statistical significance(P＜0.05).In the Logistic regression analysis:average daily working time,vigils and experiencing negative events were the risk factors of sub-health status occurrence,their odds ratio(OR) and 95 ％ confidence interval(CI)were 1.971(1.211,3.205),2.183(1.378,3.459) and 2.135(1.353,3.369),respectively.Breakfast and nutritional status were the protective factors of sub-health status occurrence,their OR and 95 ％ CI were 0.706 (0.526,0.947) and 0.386(0.239,0.625),respectively.Conclusion The unhealthy living habits and experiencing negative events affect the health of migrant workers in Dongguan City.
ABSTRACT
OBJECTIVE: To investigate the mechanism of miR-7-5p in TK6 cell damage induced by hydroquinone( HQ) by constructing stable miR-7-5p over-expressing human lymphoblastoid TK6 cell line using lentivirus. METHODS: i) The miR-7-5p over-expression lentivirus vectors were constructed,and then infected to TK6 cells. The miR-7-5p overexpression stable TK6 cell line( TK6-miR-7-5p cells) and negative control cells( TK6-NC cells) were selected with puromycin. The infection efficiency was confirmed by real-time fluorescent quantitative polymerase chain reaction assay.ii) TK6,TK6-NC and TK6-miR-7-5p cells were treated with HQ at final concentrations of 0 and 40 μmol/L for 48 hours.Cell viability was determined by CCK-8 assay. The early apoptosis rate of cells was detected by flow cytometry. The relative expression of poly( ADP-ribose) polymerase-1( PARP-1) and breast cancer susceptibility gene 1( BRCA1) proteins in 3 kinds of cells treated with HQ at the final concentration of 40 μmol/L was detected by Western blotting. RESULTS: i) The TK6 cell line with stable expression of miR-7-5p were successfully screened. Compared with normal TK6 cells,the relative expression of miR-7-5p in TK6-miR-7-5p cells increased by about 17 times( P < 0. 01) with no significant changes in cell morphology. ii) After treatment with 40 μmol/L HQ,the survival rate of TK6-miR-7-5p cells decreased compared with normal TK6 cells and TK6-NC cells( P < 0. 01),early apoptosis rate increased( P < 0. 01),the relative expression of PARP-1 and BRCA1 protein was decreased( P < 0. 05). CONCLUSION: MiR-7-5p may lead to the increase of early apoptosis in TK6 cells induced by HQ through inhibiting the DNA damage repair capacity related to PARP-1 and BRCA1.
ABSTRACT
OBJECTIVE: To explore the effect of silencing of poly( ADP-ribose) polymerase-1( PARP-1) on cell apoptosis induced by hydroquinone( HQ) in rat bone marrow-derived mesenchymal stem cells( BMMSCs). METHODS: i) The RNA expression vectors for PARP-1 gene were transfected into BMMSCs. Neomycin was used to select the transfected cells that stably expressed PARP-1-shRNA. Western blotting was used to examine the gene silencing efficiency. ii) BMMSCs with PARP-1 silencing were sorted as the treated group,while BMMSCs with empty vector were considered as the control group.HQ dissolved in phosphate buffer solution at the concentrations of 0. 0,2. 5,5. 0,10. 0,20. 0,40. 0,80. 0,160. 0 and320. 0 μmol / L were given to both groups for 24 hours. The cell viability was detected by methyl thiazolyl tetrazolium assay,and the concentration of HQ was chosen for the following study based on cell viability. iii) Both groups were treated by HQ at concentrations of 0. 0-20. 0 μmol / L for 24 hours,then the apoptosis of BMMSCs was detected by flow cytometry. The PARP-1 mRNA expression was determined by real-time fluorescent quantitative polymerase chain reaction assay. RESULTS: i) PARP-1 silencing cells and empty vector control cells were successfully screened with a mass concentration of 400 mg / L neomycin,and confirmed by level of protein expression. The interference efficiency of PARP-1 gene and inhibition efficiency was 85. 00%. ii) Based on the result of cell viability,HQ at concentrations of 0. 0-20. 0 μmol / L was chosen for the following study. iii) Compared with the group treated by HQ at concentration of 0. 0 μmol / L,the rate of early apoptosis of control group increased significantly with HQ at concentration of 10. 0 μmol / L while that of treated group was increased significantly at concentration of 5. 0 μmol / L( P < 0. 05). In addition,at concentrations of 0. 0-10. 0 μmol / L,the rates of early apoptosis in both groups increased in a dose-dependent manner( P < 0. 01). Compared with the group treated by HQ at concentrations of 0. 0 μmol / L,the expression of PARP-1 mRNA of both groups increased significantly at the concentration of 5. 0 μmol / L( P < 0. 05). The expression of PARP-1 mRNA of treated group was less than that of control group with HQ at every concentration( P < 0. 05). At concentrations of 0. 0-20. 0 μmol / L,the expression of PARP-1 mRNA of both groups increased in a dose-dependent manner( P < 0. 01). CONCLUSION: Silencing PARP-1 in BMMSCs caused cell apoptosis. PARP-1 may participate in cell apoptosis induced by HQ.