ABSTRACT
OBJECTIVE@#To study the effect of tympanostomy tube surgery in cleft palate children with secretory otitis media (SOM).@*METHOD@#Cleft palate children complicated by SOM accepted palatoplasty combined tympanostomy tube surgery, and evaluated by tympanum examination, acoustic immittance measurement in the post-operation. The others with suited age constitute, who accepted palatoplasty only,received the same testing too.@*RESULT@#Compared with children who accepted palatoplasty only,the correlated index of SOM in post-operation of children who accepted palatoplasty combined with tympanostomy tube surgery had more improvements,there was a statistically significant difference between them. The recovery degree of middle ear function in younger group was better than elder group,there was a statistically significant difference between them.@*CONCLUSION@#Tympanostomy tube surgery is helpful to increase cure rate of 0ME in children with cleft palate. The younger the patient is,the better the recovery degree of middle ear function will be. Palatoplasty combined with tympanostomy tube surgery should be an effective way in the management of cleft palate complicated by SOM, and good effect could be obtained when children accepted operation in early stage.
Subject(s)
Child , Child, Preschool , Humans , Infant , Age Factors , Cleft Palate , General Surgery , Middle Ear Ventilation , Otitis Media with Effusion , General SurgeryABSTRACT
Purpose:To study the effects and overexpression of p21 WAF1/CIP1 gene (p21 gene) on the SGC 7901 cell line plus cisplatin. Methods:An adenovirus expression vector with full length cDNA of p21 gene insert was constructed (ad p21) and transferred into SGC 7901 cell line with p53 mutation, the effects of ad p21 on the growth of SGC 7901 cell line was examined in vitro and in vivo . Results:The expression of p21 gene in SGC 7901 cell was confirmed by FCM, The growth of the Ad p21 gene transfected SGC 7901 cells was significantly inhibited as compared to mock (Ad LacZ) transfected SGC 7901 in vitro and induced the transfected cells appearing apoptotic, Combined with chemotherapeutic agent cisplatin (DDP), it could more strongly inhibit SGC 7901 cell growths.Conclusions:p21 gene can inhibit SGC 7901 cells growth and shows enhanced therapeutic efficiency for SGC 7901 cells when combined with DDP, especially in vivo experiment. Its use for gene therapy of cancer, especially when combined with other apoptosis inducing agents is promising.