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@#Objective To investigate the effect of simvastatin and mechanical pretreatment on intimal hyperplasia of venous graft and its mechanism. Methods Twelve New Zealand rabbits were selected and randomly divided into 4 groups: a blank control group, a simvastatin topical treatment group, a mechanical precondition group and a combined group (n=3 in each group). Ultrasound was used to evaluate the changes of graft wall and blood flow velocity in the graft, and pathological section was used to evaluate the intimal hyperplasia. Human umbilical cord endodermal cells were cultured in vitro. A simvastatin group and a solvent control group were set to detect YAP phosphorylation, downstream target gene expression and cell proliferation. Results Vascular ultrasound showed that except the simvastatin topical treatment group, the flow velocity in vein grafts in the other three groups significantly increased 21 days after surgery compared with 7 days after surgery (P<0.01). Pathological sections showed that the thickness of new intima in the simvastatin topical treatment group, mechanical precondition group, combined group and blank control group were 45.56±4.11 μm, 201.28±16.71 μm, 143.57±7.82 μm, 249.45±13.33 μm, respectively, and there were statistical differences compared with the blank control group (P<0.05). In vitro results showed that compared with the solvent control group, cell death was observed in high concentration simvastatin (5 mmol/L) group, cell proliferation was inhibited in low concentration simvastatin (2.5 mmol/L) group (P<0.05), the expression of YAP protein in the simvastatin group was unchanged, but the expression of phosphorylated YAP protein significantly increased (P<0.05), and the expression of downstream target gene ccn1 was down-regulated (P<0.001). Conclusion Intravascular local application of simvastatin and mechanical preconditioning alone or in combination can inhibit intimal hyperplasia of venous graft. High concentration of simvastatin has cytotoxicity, while low concentration of simvastatin has inhibitory effect on cell proliferation. Simvastatin can inhibit the formation of new intima by inhibiting the entry of YAP into the nucleus and reducing the transcription of cell proliferation-related target gene ccn1.
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Objective To evaluate the effects of group medical visits and peer education on the compliance behaviors of patients with acne vulgaris,and to explore the effective health education approaches for patients with acne vulgaris.Methods A total of 80 patients with acne vulgaris were enrolled from Hospital of Dermatology,Chinese Academy of Medical Sciences during December 2016.They were randomly and equally divided into 2 groups:intervention group receiving conventional health education combined with group medical visits and peer education,and control group receiving conventional health education alone.At week 1,2 and 4 after the intervention,the compliance behaviors were compared between the 2 groups.Results Totally,18 patients were lost to the follow-up due to refusals and non-response,and 32 patients in the intervention group and 30 patients in the control group finally completed the study.At week 1,2 and 4 after the intervention,the scores of compliance behaviors were significantly higher in the intervention group (83.6 ± 9.3,85.9 ± 9.1,91.2 ± 8.4 respectively) than in the control group (77.1 ± 7.3,77.1 ± 8.6,79.1 ± 10.2 respectively;all P ＜ 0.05).Moreover,the scores of compliance behaviors significantly increased over time (P ＜ 0.05),and there was a significant interaction effect between the intervention methods and treatment duration (P ＜ 0.05).Conclusion Health education approaches including group medical visits and peer education can improve the compliance behaviors of patients with acne vulgaris.
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OBJECTIVE: To study the intervention effects of participatory training on the occupational health in polishing workers. METHODS: By the stratified random sampling method,930 front-line polishing workers were selected from 50 manufacturing enterprises in Guangzhou,467 workers in the intervention group and 463 workers in the control group. The intervention group adopted participatory training and the control group adopted the traditional training. We evaluated the changes of occupational health knowledge,attitude and practice( KAP) in workers of these two groups using the questionnaire table of occupational health KAP for polishing workers. Data of before intervention,immediately after intervention and 3 months after intervention were collected. RESULTS: The workers 'knowledge,attitude and practice scores at the time point of immediately after intervention were higher than those before intervention in the same group( P <0. 05). The workers' knowledge and practice scores of 3 months after intervention in intervention group were higher than those before intervention( P < 0. 05). The knowledge and practice scores of 3 months after intervention in the intervention group were higher than that in the control group( P < 0. 05). The evaluation scores on protection measure and warning signs of workers in the intervention group were lower than those in workers of control group 3 months after the intervention( P < 0. 05). Three months after the intervention,the workers in intervention group believed that the training was more effective than that in the control group in the following 6 aspects: improving the knowledge of occupational health,improving the ability of identifying risk factors, learning to use the personal protective equipment, helping other workmates,having confidence to make suggestions and introducing other workmates to participate in training( P < 0. 05).CONCLUSION: The participatory training is an effective intervention model in improving the workers ' awareness of occupational health KAP.