ABSTRACT
AIM: To establish a HPLC for the determination of rubimaillin in Shenqiangujing Granules (Radix Codonopsis, Radix Rubiae, Radix Rehmanniae, Fructus Ligustri Lucidi, etc.) METHODS: HPLC column was C-18 (250 mm?4.6 mm, 5 ?m). The mobile phase was methanol-water-tetrahydrofuran (310∶90∶3). Flow rate was 1.0 mL?min -1 . The detection wavelength was at 249 nm. RESULTS: The linear range of rubinaillin was in the range of 0.056～0.29 ?g, r=0.9999. The average recovery was 99.36% (RSD=1.60%, n=5). CONCLUSION: The method is accurate with the good reproducibility, and can be used for the determination of rubimaillin in Shenqiangujing Granules.
ABSTRACT
AIM: To estahlish a RP-HPLC method of determining two caffeic acid derivatives in Echinacea extract and to determine 5 Echinacea purpurea root extracts. METHODS: RP-HPLC was applied to determine 2 caffeic acid derivatives in E.purpurea extract:caftaric acid and chicoric acid.HPLC conditions were as follows: Agilent ZORBAX StableBond C_(18) column(5 ?m,4.6 mm?250 mm) was used,with A(acetonitrile) —B(0.1% H_3PO_4) gradient elution as a mobile phase.The flow rate was 1.2 mL/min.The detection wavelenth was set at 330 nm. RESULTS: 2 caffeic acid derivatives were separated well.The linear range for caftaric acid and chicoric acid were 0.064-0.416 ?g and 0.1-0.7 ?g,respectively.Its average recoveries were 99.37% with RSD of(1.50%) and 100.44% with RSD of 1.71%,respectively. CONCLUSION: The method is simple,accurate,strong specificity and can be used to control the quality of E.purpurea extract and new medicine development.