ABSTRACT
Objective:To investigate the protective effects of Compound Danshen Injection and Benazepril on rats with obstructive nephrosis.Methods:The interstitial nephritis model of rat was established by ligating unilateral ureter. The model rats were given drug Compound Danshen Injection and Benazepril, respectively. The levels of angiotensin Ⅱ (Ang Ⅱ), endothelin (ET 1) and the activity of angiotensin converting enzyme (ACE) in renal tissues were measured. Results: The level of angiotensin Ⅱ of Compound Danshen Injection group was 42.03?13.95pg/mg lower that of pathologic group (80.19?27.31pg/mg). The level of Benazepril group was 32.53?8.07pg/mg. There were significant differences among them. The measuring results of ACE and ET 1 were similiar to that of Ang Ⅱ.Conclusion: Compound Danshen Injection and Benazepril can effectively inhibit the activity of ACE and decrease the levels of Ang Ⅱ and ET 1.
ABSTRACT
Aim To observe the changes on the expression of ?-SMA in rats with adriamycin-induced nephrothy and intervention of Valsartan. Methods The model group were made by unilateral nephrectomy with twice-adriamycin injections by caudal vein. Immunohistochemical method, flow cytometry and pathologic image analysis were used to observe the expression of ?-SMA, FN and LN in rats. Results The expression of ?-SMA was positive in the model group. Rates of positive cell and protein semi-quantitative analysis were more higher than the sham group(P