Mercury accumulation of Tibetan medicine Zuotai in mice / 中成药

AIM To explore the mercury accumulation in KM mice after being given Zuotai at different doses and time.METHODS KM mice were randomly divided into blank group,Zuotai low-,middle-and high-dose (6.07,60.70 and 606.97 mg/kg,42 d;606.97 mg/kg,14 d) groups.The mercury contents in brain (olfactory bulb,cortex,hippocampus,hypothalamus,brain stem,cerebellum),heart,lung,kidney,liver,spleen,serum,muscle of mice were measured after administration.RESULTS Compared with the blank group,Zuotai at low-dose significantly increased the mercury contents in hippocampus,cerebellum,lung,kidney,liver and serum of mice after 42-day treatment;Zuotai at middle-dose markedly increased the mercury contents in olfactory bulb,cortex,hippocampus,brain stem,cerebellum,heart,lung,kidney,liver,spleen and serum of mice after 42-day treatment;the mice treated with high-dose of Zuotai for 42,14 days significantly increased the mercury contents in olfactory bulb,cortex,hippocampus,hypothalamus,brain stem,cerebellum,heart,lung,kidney,liver,spleen,muscle and serum.CONCLUSION Mercury can be accumulated in different tissues of mice after intragastric administration of Zuotai in a dose-and time-dependent manner,which suggests that Zuotai and its compound preparations should not be used in high-dose and long-term.

Effects of Atorvastatin with Different Doses on Related Indicators and MACE in Patients with Acute Myocar-dial Infarction after PCI / 中国药房

OBJECTIVE:To investigate the effects of atorvastatin with different doses on related indicators and major adverse cardiovascular events(MACE)in patients with acute myocardial infarction(AMI)after percutaneous coronary intervention(PCI). METHODS:120 patients with AMI after PCI were randomly divided into conventional dose group(60 cases)and high dose group (60 cases). All patients were immediately given low molecular weight heparin,Aspirin enteric-coated tablet,Clopidogrel bisulfate tablet,Angiotensin-converting enzyme inhibitor,β-blockers,nitrates and other conventional treatment;based on it,conventional dose group was given 20 mg Atorvastatin calcium tablet,once every day before bedtime;high dose group was given 40 mg Atorv-astatin calcium tablet,once every day before bedtime. The treatment course for both groups was 1 month. Total cholesterol(TC), triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),C-reactive protein (CRP),OX40L,interleukin (IL)-6 levels in 2 groups before and after treatment were observed,the correlation among OX40L with CRP and IL-6 were analyzed,and the incidence of MACE in 2 groups was recorded after 1 month follow-up. RESULTS:Be-fore treatment,there were no significant differences in the TG,TC,LDL-C,HDL-C,OX40L,CRP and IL-6 between 2 groups (P>0.05). After treatment, TG, TC, LDL-C, OX40L, CRP and IL-6 were aignificantly lower than before, TC, LDL-C, OX40L,CRP and IL-6 in high dose group were lower than conventional dose group,HDL-C was significantly higher than before, the differences were statistically significant(P0.05). Before treatment,OX40L showed positive correlation with CRP(r=0.746,P<0.01)and IL-6(r=0.763,P<0.01);after treatment,OX40L also showed positive correlation with CRP (r=0.755,P<0.01) and IL-6 (r=0.760,P<0.01). The incidence of MACE in high dose group were significantly lower than conventional dose group,the difference was statistically significant(P<0.05). CONCLUSIONS:Based on the conventional treatment,atorvastatin can significantly reduce lipid,inflamma-tion levels and incidence of MACE,but the effect is more significant in high dose.

Chemical and structural analysis of Nengchi Bajin ashes in refining of Tibetan medicine gTSo thal / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical components and microstructure of Nengchi Bajin ashes which are adjuvant material in the refining of Tibetan medicine gTSo thal, in order to explore the material basis of the refining of gTSo thal.</p><p><b>METHOD</b>Scanning electron microscope-energy dispersive spectrometer (SEM-EDX) and X-ray diffraction (XRD) were used to measure the Nengchi Bajin ashes.</p><p><b>RESULT</b>SEM-EDX analysis show that except of themselves elements of Nengchi Bajin ashes, Nengchi Bajin ashes contain the major elements, such as S, O, C and so on, also contain small amount other elements. XRD analysis show that the structures are AuPb2, PbO (tetragonal and orthorhombic) and Pb in gold ash, Ag2S and PbO in silver ash, Cu1.98 (Zn0.73 Fe0.29)Sn0.99 S4, CuS, SiO2, NaCu2S2 and Ca (Fe(+2), Mg) (CO3)2 in bronze ash, Cu7S4 (orthorhombic and monoclinic) and CuO in red copper ash, Cu7 S4, PbS, ZnS, CaCO3and NaCu2S2 in brass ash, FeS, Fe+2 Fe(2+3)O4 and SiO2 in iron ash, SnS and SiO2 tin ash, PbS, PbSO4 and SnS2 in lead ash.</p><p><b>CONCLUSION</b>We have acquired the datum of elements and microstructure of Nengchi Bajin ashes by SEM-EDX and XRD techniques, and that is benefit to explore the material basis of refining gTSo thal.</p>

HPLC determination of four components in Tibetan medicine Dangzuo of different Tibetan regions / 中国中药杂志

<p><b>OBJECTIVE</b>To develop an HPLC method for determination of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine in Tibetan medicine Dangzuo, and to compare the content of four active components in Dangzuo of different Tibetan regions.</p><p><b>METHOD</b>The separation was carried out on a Waters XTerra RP-C18 column ( 4.6 mm x 250 mm, 5 microm). The mobile phases were methanol and water, all contained 0.1% glacial acetic acid, for gradient elution. The gradient program was as follows: 0-22.5 min, methanol was changed from 5% to 50%; 22.5-40 min, changed to 80% 80:20. The flow rate was 1.0 mL x min(-1). The detection wavelength was 270 nm. The reference wavelength was 500 nm.</p><p><b>RESULT</b>The linear ranges of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine were 0.040-0.640 microg (r = 0.999 8), 0.090-1.440 microg (r = 0.999 9), 0.031-0.500 microg (r = 0.999 9 ) and 0.092-41.477 microg (r = 0.998 9), respectively. The average recoveries (n = 6) were 97.42% (RSD 1.9%), 97.55% (RSD 2.9%), 98.69% (RSD 0.96%) and 96.72% (RSD 4.0%), respectively. The content ranges of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine in Dangzuo samples of different Tibetan regions were 0.11341.69 mg x g(-1), 0.889-1.51 mg x g(-1), 0.000-40.606 mg x g(-1) and 1.96-2.73 mg x g(-1), respectively.</p><p><b>CONCLUSION</b>The method is a simple and effective for quality control of Tibetan medicine Dangzuo.</p>

Study on processing technics of Tibetan medicine Nanhanshuishi / 中国中药杂志

<p><b>OBJECTIVE</b>To study the heat processing technics of Nanhanshuishi.</p><p><b>METHOD</b>To find the best processing technic, the single factor experiments and orthogonal experiments were designed basing on the processing technics summarized by consulting documents, scriptures and investigating some Tibetan hospitals, meanwhile, the content of Ca, Fe, Mn, Zn, and Cu in the processed Nanhanshuishi in single factor experiments and orthogonal experiments were detected.</p><p><b>RESULT</b>The best processing technic of Nanhanshuishi was as follows: Nanhanshuishi was crashed to 10-20 mm in diameter, the ratio of the weight of Aconiti Kusnezoffii Radix and potassium nitrate was 1: 2, and the boiling time was 3 h.</p><p><b>CONCLUSION</b>The work in this article provided a basic processing technic data for clarifying the mechanism of processing and establishing the perfect processing technics of Nanhanshuishi.</p>

Composition and thermal stability of traditional Tibetan mineral medicine Nanhanshuishi / 中国中药杂志

<p><b>OBJECTIVE</b>To determine the composition, structure, trace elements and thermal stability of Tibetan medicine Nanhanshuishi.</p><p><b>METHOD</b>The trace elements, the structure, and the thermal stability of Nanhanshuishi were assayed and calculated by X-ray fluorescence spectrometry (XRF), inductively coupled plasma optical emission spectrometer (ICP-OES), atomic fluorescence spectrometry (AFS), inductively coupled plasma mass spectrometry (ICP-MS), X-ray power diffraction (XRD), TG-DTA.</p><p><b>RESULT</b>The results indicated that the phase is mainly made up of CaCO3 (Rhombohedral, R-3c) in Nanhanshuishi. The analysis of elements show that Nanhanshuishi is rich in Ca and O, and contains other more than 20 minor elements, such as Si, Mg, Fe, Al, Na, K, Zn, Mn, Pb, As, Hg etc. The result of TG-DTA show that the weight of Nanhanshuishi starts to decline from near 700 degrees C and get steady above 850 degrees C.</p><p><b>CONCLUSION</b>The study provided scientific data for the establishment of quality standards of Tibetan medicine Nanhanshuishi.</p>

Preparation process for Tibetan Anshen Pills with supercritical fluid extraction / 中成药

Objective: To explore the preparation process for Tibetan medicine Anshen Pills. Methods: Comparing the preparation process of Tibetan medicine extracted by SFE CO 2 with that extracted by pertroleum ether and analyzing chemical components of their extracts by GC/MS. Results: The method of SFE CO 2 is superior to extractive method by pertroleum ether. The former has higher extraction ratio and shorter extraction time without residues of chemical solvent. Conclusion: The process of SFE CO 2 is suitable for the preparation of Tibetan medicine Anshen Pills.

Chemical components analysis by GC-MS of supercritical-CO_2 extracts from Tibetan medicine Pazhu Capsule / 中成药

AIM:To study the main chemical components of traditional Tibetan medicine Pazhu Capsule(Calcitum,Fructus Chebulae,Semen Granati,Fructus Piperis,Fructus Piperis Longi,etc.) in the treatment of gastropathy. METHODS: The soluble components of Pazhu Capsule were extracted by supercritical-CO_2 fluid.The components were measured by GC-MS and the relative amount of them were determined by peak area normalization.(RESULTS:)22 components were identified.The main components were piperine(44.2%),9,12-Octadecadienoic acid(z,z)(21.1%),Azuleno[45-b] furan-2(3H)-one,3a,4,6a,7,8,9,9a,9b-octahydro-6-methyl-3,9-bis(methylene)-,[3as-(3a.alpha.,9a.alpha.,9b.beta.)](10.55%),n-Hexadecanoic acid(4.8%) and 2(3H)-Benzofuranone,6-ethenylhexahydro-6-methyl(33.5%).CONCLUSION: The main components of the Tibetan medicine Pazhu Capsule are piperine and 9,12-Ocatadecadienoic acid,both contents are up to 65.3% of the total extracts.