Alginate extraction of Pseudomonas aeroginosa and study of penetration of antibiotic agents through alginate using a sandwich cup method

Pseudomonas aeroginosa is one of the most important opportunistic bacteria which produces biofilm. It has been reported that biofilm forming bacteria are more resistant to antibiotic treatment and immunologic response. Biofilms, communities of cells adhering to a surface enclosed in a self-producing polymeric matrix. Biofilms might be responsible for 65%of bacterial infections. In this study we investigated the role of the barrier formed by alginate against antibiotic penetration .Mucoid isolates of P. aeruginosa were collected from 2 hospitals and identified by biochemical tests. Strain M214 P. aeruginosa produces more biofilm compared with other strains. Alginate was extracted from mucoid type P. aeruginosa [M214] with method described by Goven. We evaluated the penetration of antibiotics macrolides [azithromycin,erythromycin],aminoglycosides [amikacin,gentamicin] lactames [imipenem,ceftazidem],fluoruqinolones [ciprofloxacin,ofloxacin] through P. aeruginosa [M214] alginate with a sandwich cup method described by Hiromi Kumon indicated that macrolides demonstrated penetration rate 100% .fluroquinolones and lactames demonstrated relatively high penetration rate>85%, whereas aminoglycosides showed low penetration [amikacin=59%,gentamicin=73%]. In this study, the role of alginate as a barrier against antibiotics penetration was proven

[Alginate extration of Pseudomonas aeroginosa and study of penetratuion of antibiotic agents through alginate using a sandwich cup method]

Background: P seudomonas aeroginosa is one of the most important oppourtunistic bacteria which produces biofilm. It has been reported that biofilm forming bacteria are more resistant to antibiotic treatment and immunologic response. Biofilms, communities of cells adhering to a surface enclosed in a self-producing polymeric matrix. Biofilms might be responsible for 65% of bacterial infections

Materials and methods: In this study we investigated the role of the barrier formed by alginate against antibiotic penetration. Mucoid isolates of P. aeruginosa were collected from 2 hospitals and identified by biochemical tests. Strain M2 14 P. aeruginosa produces more biofilm compared with other strains. Alginate was extracted from mucoid -type P. aeruginosa [M2 14] with method described by Gaven. We evaluated the penetration of antibiotics macrolides [azithromycin, erythromycin], aminoglycosides [amikacin, gentamicin] lactames [imipenem, ceftazidem], fluoruqinolones [ciprofloxacin, ofloxacin] through P. aeruginosa [M214] alginate with a sandwich cup method described by Hiromi Kuman

Results: The results indicated that macrolides demonstrated penetration rate 100%, fluroquinolones and lactames demonstrated relatively high penetration rate>85%, whereas aminoglycosides showed low penetration [amikacin=59%, gentamicin=73%]

Conclusions: In this study, the role of alginate as a barrier against antibiotics penetration was proven