[ protective effect of green tea [Camellia sinensis] extract on kidney tissues and blood factors of kidney functions in male mice treated with paclitaxel]

Background: In the world of today, where the diseases are on the rampage, doctors and patients both are trying to diagnose and treat them, however, the difficulty is ignoring some causes in diagnosis and even treatments. Paclitaxel is a treatment chemical drug that can cause kidney toxicity, including degenerative changes in the renal parenchyma

Objectives: The aim of this study was investigated the effects of green tea as a strong antioxidant on kidney tissue and blood factors of kidney functions in mice treated with paclitaxel

Methods: Doing this experimental 35 mice divided into7groups randomly: 1 control group was not achieved any materials,2control group achieved Saline for one week,1 Shame group were injected with Paclitaxel IP of 0.3 mg/kg for three days; 2and 3 Shame group injected with IP of green tea with doses of 200 mg/kg and 300 mg/kg respectively for twenty-eight days.1 and 2 Experimental group went through treatment with green tea extract and paclitaxel, they first achieved said doses of green tea for twenty-eight days and then paclitaxel with the dosage of 0.3 mg/kg from the fourteenth day for three days. Seven days after the last injection, the kidney was dissected and after fixation in Formaline buffer solve, have been painted with haematoxylin and eozine, and was studied. Also the level of blood urea and creatinine has been investigated. Data was analyzed by ANOVA test

Results: Blood urea and creatinine levels in 1Shame group were significantly increased compared with the control group the after using green tea extract in 1 and 2 Experimental group showed a significant decrease[P<0.05]. In 1Shame group, dilatation of the renal collecting tubular with minor tissue necrosis was observed the trend in 1 and 2 Experimental group to be reversible so that the control group had no significant difference

Conclusion: On the Base of these results, consuming green tea was decreased of the level of blood factors of kidney and the kidney tissue changes brought about by paclitaxel will be improved

Feeding effect of cholesterol in the diet on sex hormones concentrations and the gonads' growth of yearling common carp [Cyprinus carpio]

There are some evidences that cholesterol can affect fish reproductive system. In this study the effect of high diet cholesterol on fish sex hormones and gonads weight was investigated. A randomized experiment was conducted on 90 immature common carp. The fishes were divided into three groups with three replicates each [control, 0.5% cholesterol diet, and 1% cholesterol diet]. After one-month feeding, progesterone, estrogen and testosterone were measured. The level of progesterone in control group, 0.5% cholesterol and 1% cholesterol diet were 1.83 +/- 0.42, 2.45 +/- 0.38 and 2.62 +/- 0.52 ng/ml, respectively. Also the level of estrogen in control group, 0.5% and 1% cholesterol diet were 3670.34 +/- 186.26, 3791.20 +/- 98.48, and 3836.78 +/- 81.74 pg/ml, respectively., while those of testosterone levels were 1.75 +/- 0.319, 2.09 +/- 0.425, and 2.25 +/- 0.321 ng/ml, respectively. The highest and the lowest body weight and gonad weight were observed in fish fed% 1 cholesterol and the control, respectively. The results showed positive effects of cholesterol on sexual hormones, the 0.5% cholesterol diet and the 1% cholesterol diet did not show any significant difference in the sexual hormones levels. The results indicated that the effect of cholesterol on the these sex hormones, gonad weight, body length and body weight was significant [p<0.01]. We can conclude that diets of 0.5% cholesterol can be used to increase the amount of sexual hormones in common carp