effect of somatostatin analogue, octreotide, on the jejunal mucosa of bowel resected albino rat

The aim of study was to demonstrate the effect of this drug on the histological and histochemical structure of the intestinal mucosa after small intestinal resection. 60 albino rats weighting 250 +/- 50 g were divided into 3 groups, 20 for each. Group A animals were used as controls, while the animals of groups B, C were subjected to resection of 10 cm of the jejunum. The rats of group C were also subjected to postoperative injection with sandostatin [2 mug/kg s.c. bid] for 14 days. The animals were sacrificed 2 weeks after the operation, paraffin sections from the jejunum were processed and stained with Hx, E, PAS, Grimellus technique for neuroendocrine cells and Gomori's method for alkaline phosphatase. Jejunal sections of group B showed that the mean length and breadth of jejunal villi were significantly increased with hyperplasia of the covering epithelium and marked significant increase in goblet and neuroendocrine cell populations. The brush borders of absorptive cells was more prominent with marked increase of its alkaline phosphatase activity [these changes were statistically significant as compared with group A]. Jejunal sections of group C showed that all the previous results were reversed as compared with group B [statistically significant], but insignificantly changed as compared with group A

hazardous effects of the red food colouring substance [new-coccine] on the liver of albino rats

The addition of artificial color to food and sweet, consumed by school children, is a field of great health concern in Egypt. The red coloring substance [new coccine] was added to the food and water of albino rats for 6 months. Paraffin sections from the liver of the experimental animals were stained with Hx, E, and PAS reaction. Frozen sections were stained for evaluation of acid phosphatase and succinic dehydrogenase activities. Ultra thin sections for transmission E/M study were also prepared. Hx, E and PAS stains showed disturbance of the architecture of the liver lobules, cellular degeneration, vacuolation of the hepatocytes and depletion of glycogen. Increased acid phosphatase and decreased succinic dehydrogenase activities appeared in frozen sections which were treated for these enzymes. Transmission, E/M sections showed swelling of the mitochondria, rupture of their membranes and cristae