ABSTRACT
@#[摘 要] 目的：探讨长基因间非编码RNA 00519（long intergene non-coding RNA 00519，LINC00519）调控miR-876-3p/高迁移率家族蛋白A1（high mobility group protein A1，HMGA1）轴在胃癌HGC-27细胞的增殖、凋亡、迁移和侵袭中的作用。方法：采用qPCR检测胃癌细胞HGC-27和胃黏膜上皮细胞GES-1中LINC00519的表达水平。将HGC-27细胞按转染处理分为si-NC、si-LINC00519、si-LINC00519+anti-miR-NC和si-LINC00519+anti-miR-876-3p组，采用集落形成实验检测细胞克隆形成能力，流式细胞术检测细胞凋亡和周期分布，Transwell实验检测细胞迁移和侵袭。双荧光素酶报告实验和qPCR验证LINC00519与miR-876-3p、miR-876-3p与HMGA1之间的相互作用。结果：HGC-27细胞中LINC00519表达较GES-1细胞显著升高（P<0.05），转染siRNA后si-LINC00519组HGC-27细胞中LINC00519的表达水平较si-NC组显著降低（t=47.294，P<0.01）。与si-NC组比较，si-LINC00519组HGC-27细胞克隆数、迁移侵袭数、S期细胞比例均显著降低（均P<0.01），凋亡率、G0/G1期细胞比例均显著升高（均P<0.01）。与si-LINC00519+anti-miR-NC组比较，si-LINC00519+anti-miR-876-3p组HGC-27细胞克隆数、迁移侵袭数、S期细胞比例升高（均P<0.01），凋亡率、G0/G1期细胞比例显著降低（均P<0.01）。LINC00519能够靶向负调控miR-876-3p的表达，miR-876-3p靶向负调控HMGA1的表达。结论：敲降LINC00519能够通过调控miR-876-3p/HMGA1轴抑制胃癌HGC-27细胞的增殖、迁移和侵袭，诱导细胞凋亡。
ABSTRACT
AIM:To perform a clinical analysis of 1 500 cases of outpatients with ametropia in Hebeisheng Eye Hospital,to provide a theoretical basis for diagnosis,treatment,and prevention of patients with ametropia.METHODS:Totally 1500 cases (2840 eyes) of outpatient with ametropia were chosen as the research objects in Hebeisheng Eye Hospital from June 2013 to July 2014.All cases were treated with TOPCON RM-8800 computer optometry instrument for objective optometry,and used TOPCON phoropter for subjective optometry.The combination of the two instruments was taken to determine the diopter later.Diopter and axial distribution of the myopic astigmatism and hyperopic astigmatism were observed,age of astigmatic patient,distribution of astigmatism axis were observed as well.Meanwhile,a total of 150 cases were chosen randomly to measure their corneal curvature,anterior chamber depth,axial length and other static refractive index.All indexes were compared with those of the normal people subsequently,such as amplitude of accommodation,negative relative accommodation,positive relative accommodation,sensitivity of accommodation and other dynamic refractive index.RESULTS:Ametropia types of all patients were mainly simple myopia,simple myopia astigmatism,compound myopic astigmatism,simple hypermetropia,simple hyperopia astigmatism,compound hyperopic astigmatism and mixed astigmatism,the proportion were 38.99％,3.27％,23.94％,4.68％,1.34％,13.52％,15.25％ respectively.There were 773 eyes with myopia astigmatism.The proportion of people with a myopia astigmatism diopter of above-0.25 to-0.50,-0.75 to -1.00,-1.25 to-1.50,above-1.75 were 31.05％,38.55％,16.56％,13.84％.There were 421 eyes with hyperopia astigmatism,the proportion of people with hyperopia astigmatism diopter of 0.25-0.50,0.75-1.00,1.25-1.50,＞1.75 were 26.60％,24.94％,16.63％,31.83％.Static refractive index of 150 patients (300 eyes) showed that corneal curvature was (41.23 ± 2.43) φ/D,anterior chamber depth was 3.71 ± 0.43mm,axial length 23.45 ± 1.43mm.Dynamic refractive index showed that the amplitude of accommodation 10.56±2.32D,negative relative accommodation 2.31 ±0.47D,positive relative accommodation-1.82-± 0.67D,sensitivity of accommodation 11.34±2.21D.All kinds of static and dynamic refractive indexes,the length of ocular axis were statistically different from those of the normal population (P ＜ 0.05).Regular astigmatism number rate in 1 194 cases of astigmatism eyes of 3-7 years old,8-18 years old,19-45 years old,46-60 years old were 35.85％,11.98％,45.64％,6.53％.Among 150 cases of patients (300 eyes),there were 152 eyes of equivalent sphere mirror ＜-0.5D,48 eyes of equivalent sphere mirror ≥ 0.5D,100 eyes of equivalent sphere mirror between-0.5D to 0.5D.And there were 150 eyes of equivalent sphere mirror ＜-0.5D,50 eyes of equivalent sphere mirror ≥0.5D,100 eyes of equivalent sphere mirror between-0.5D to 0.5D in a total of 300 eyes of 150 normal people.CONCLUSION:The 1500 cases of patients with ametropia (2 840 eyes) contains mainly simple myopia and compound myopic astigmatism,diopter range in myopia astigmatism were chiefly-0.25 to-0.50,-0.75 to-1.00,hyperopia diopter range consists of ＞ 1.75 mostly,among the patients with astigmatism,the older the age,the bigger the number of people with irregular astigmatism,static and dynamic refractive index of patients with ametropic eye shows apparent difference with that of the normal population.