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This study aimed to investigate the effect and possible mechanism of carnosic acid (CA) on delaying aging. The effects of CA on senescence-related β-galactosidase (SA-β-Gal) activity and expressions of p53, p21 and p16 were evaluated by an oxidative challenge induced premature 2BS cell senescence model. Meanwhile, the animal experiment was approved by the Ethics Committee of Zhejiang Hospital. Male C57 BL/6J mice were injected with 100 mg·kg-1·d-1 D-galactose (D-gal) for 8 weeks to establish an aging model in vivo, and CA at 5 and 10 mg·kg-1·d-1 were given ig administration at the same time. Morris water maze test was used to test the spatial memory ability. Then the serum and tissue samples were collected for the detections of malondialdehyde (MDA), total superoxide dismutase (T-SOD), interleukin-6 (IL-6), tumor necrosis factor α (TNFα) and advanced glycation end products (AGEs) as well as the protein expression of p53, p21 and p16 in hippocampus of brain. The results showed that H2O2 induced increment of SA-β-Gal activity (95%) was prevented by CA treatment (35%) and the enhanced protein expressions of p53, p21 and p16 in H2O2 exposed 2BS cells were alleviated by CA treatment, suggesting a potent protective role of CA against premature senescence induced by oxidative challenge. For in vivo study, D-gal induced declined spatial memory ability was partly reversed by CA administration. Besides, the serum and cerebral levels of MDA, IL-6, TNFα and AGEs were attenuated by CA treatment when compared to those in model mice. And the protein expressions of p53, p21 and p16 in mice hippocampus were suppressed by CA in D-gal treated mice. Taken together, our results showed that CA protects premature senescence induced by oxidative stress and D-gal, which is related to its antioxidative, antiinflammatory roles and inhibition on non-enzymatic glycosylation.
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Objective: To explore the application of the cuff-leak test(CLT)guiding offline extubation in patients with tracheal intubation of mechanical ventilation.Methods: 64 patients with tracheal intubation who underwent mechanical ventilation were divided into CLT-negative group(47 cases)and CLT-positive group(17 cases)according to leakage situation of CLT.The CLT guiding offline extubation were adopted in the study and some basic situations,such as body mass index(BMI),APACHE-Ⅱ and so on,of the two groups were compared.And the relative situation of intubation,blood gas analysis index,vital signs,the occurrence rate of upper airway obstruction(UAO)post removing intubation and re-intubation rate between the two groups also were compared,and then the risk factors of influencing UAO were further analyzed.Results: The BMI of CLT-negative group was significantly smaller than that of CLT-positive group(t=2.44,P＜0.05).The occurrence rate of UAO and re-intubation rate of CLT-negative group(6.38％and 2.13％)were significantly lower than that of CLT-positive group(35.29％and 17.65％)(x2=8.63,x2=5.13,P＜0.05),respectively.The differences of BMI,APACHE-Ⅱ scores,intubation time,air sac pressure,PaO2and SpO2between patients with UAO and patients without UAO were significant(t=5.63,t=2.65,t=4.27,t=3.35,t=2.37,t=2.66,P＜0.05).The results of Logistic regression analysis showed that the BMI,APACHEⅡ score,intubation time,air sac pressure were independent risk factors for occurring UAO post extubation.Conclusion: The CLT guiding offline extubation in patient with tracheal intubation of mechanical ventilation can effectively reduce the re-intubation rate.For these patients with obesity,high APACHE-Ⅱ score,long intubation time and big air sac pressure,the risk of occurring UAO is higher.Therefore,the number of intubation pre extubation should be reduced for them,and their physiological status should be comprehensively assessed so as to decrease the occurrence rate of UAO.
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Objective To investigate the relationship between the driver genes mutation and the survival in patients with pulmonary adenocarcinoma brain metastasis. Methods We enrolled 200 patients with pulmonary adenocarcinoma brain metastasis confirmed histologically from Jan 2013 to Dec 2015, and tested EGFR, KRAS, ALK, Her-2 gene mutation, analyzed the relationship between EGFR gene mutation and clinicopathological data and prognosis of the patients.Results The mutation rates of EGFR, KRAS, ALK and Her-2 gene mutation were 48.5％, 5.5％, 6.5％, 3.5％, respectively. Compared with EGFR gene mutation patients, the sex, age, BMI, differentiation were significant different (P＜0.05), however, the smoking was not significant different (P＞0.05). Patients with EGFR gene mutation who received targeted therapy survived longer than who did not receive targeted therapy, (28.0 ±4.5) months vs (11.2 ±1.4) months. By Log Rank (Mantel-Cox), the median survival time between the two groups was statistically significant (P＜0.05).Conclusions The mutation rate of EGFR gene mutation was high in patients with pulmonary adenocarcinoma brain metastasis, and the patients will survivel longer by targeted therapy.
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AIM:To compare the efficacy and safety of intravitreal ranibizumab to those of triamcinolone acetonide ( TA ) injection for the treatment of macular edema secondary to central retinal vein occlusion ( CRVO) . METHODS:This retrospective study included 40 eyes of 40 patients with macular edema associated with CRVO. Twenty patients 20 eyes were treated with intravitreal injection of triamcinolone acetonide (1mg, 0. 1mL), the other 20 patients 20 eyes accepted intravitreal ranibizumab (0. 5mg, 0. 05mL). The change of best corrected visual acuity ( BCVA ) , central macular thickness ( CMT ) , and intraocular pressure ( IOP ) before treatment and at 1, 2wk, 1, 2,3,6mo post-injection in the two groups were observed. RESULTS:BCVA was improved at 1, 2wk, 1, 2,3,6mo post-injection in the TA group (P 0. 05 ). CMT decreased significantly within each group ( P 0.05). In the TA group, the IOP was significantly higher at 2wk and 4wk than before treatment (P0. 05). However, the IOP at 1mo was significantly higher in the TA group than that in the ranibizumb group (P CONCLUSION:Intravitreal ranibizumab is an effective and safe treatment method for macular edema secondary to CRVO. It can effectively improve BCVA and reduce CMT without ocular and systemic complications compared with intravitreal TA.
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Burns and traumas are common injuries during both peace time and wartime. Lung is the earliest organ subjected to dysfunction and the incidence is highest. The systemic protective technology for the burn and trauma related lung injuries is based on evidence-based medicine and translational medicine. It includes a series of effective measures, such as rescue and treatment scheme for massive burn casualties, prophylactic tracheostomy, protective ventilation strategy, sequential cell protection, and prevention and treatment of sequelae, which prevents aggravation of lung injuries caused by ischemia reperfusion, oxidative stress, and iatrogenic factors, as well as reduces the incidence of complications to ensure the recovery after burns and traumas.
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Humans , Burns , Evidence-Based Medicine , Lung Injury , Translational Research, BiomedicalABSTRACT
Previous researches showed that the expression level of E-Cad in most infiltrating cancer cells was reduced or negative.This study explored whether 4HPR restrained the infiltration of bladder cancer cells through regulating the expression of E-Cad.The infiltrating bladder cancer cells T24 were cultured,and then treated by a proper dosage of drug.Their viability was a determined by MTT method.Western blotting and RT-PCR were adopted to detect the changes of E-Cad gene expression at both protein and mRNA levels.Moreover,immunofluorescent staining and confocal fluorescence microscopy were employed for the observation of the expression of E-Cad.The result showed that,at both mRNA and protein levels,the expression level of E-Cad in T24 cells treated by 4HPR was significantly higher than that of control group,while the β-Cat expression was also relocated from the cell nucleus to cytoplasm.Our findings suggested that the regulatory function of 4HPR on infiltration of bladder cancer cells T24 is at least partly achieved by regulating the expression of E-Cad.
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This paper is dedicated to evaluate the thermal behavior of skin surface embedded with tumor tissue through construction of three-dimensional heat transfer model of the human body. It was found that the far-infrared imaging equipment could not yet get the accurate results for diagnosis of tumors developed in early stage or located deeply in the human body, because of limited resolution and accuracy in the current system. Conceptual experiments with a thermal imaging system under various cooling levels were performed to confirm this issue. A dual cooling cavity was proposed to realize ultra-low-temperature so as to improve the cooling of the current infrared equipment and thereby to enhance its image precision and accuracy. This study is expected to be of significant reference value for realizing an early diagnosis of cancers through medical image.
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Diagnostic Imaging , Methods , Early Detection of Cancer , Methods , Early Diagnosis , Infrared Rays , Neoplasms , DiagnosisABSTRACT
The action mechanism of matrix metalloproteinases-2 (MMP-2) and tissue inhibitor of metalloproteinases-2 (TIMP-2) in the genesis, development and degeneration of haemangioma was in-vestigated by detecting their expression in the tissue of haemangioma in different phases by using the immunohistochemistry. Fifty paraffin-embedded specimens of skin capillary haemangioma were col-lected, which were documented in the Department of Pathology, Renmin Hospital of Wuhan University from 2000 to 2006. All samples were stained by regular HE method, and proliferative cell nuclear anti-gen (PCNA) was tested by immunohistochemical S-P method, The samples were classified according to the Mulliken criteria and the expression pattern of PCNA. Immunohistochemical S-P method was ap-plied to detect the expression of MMP-2 and TIMP-2 in proliferative and degenerative phases of cuta-neous capillary haemangioma, and in normal skin tissues. In combination with the detection of the ex-pression of factor Ⅷ-related antigen, it was verified that in haemangioma tissues, the cells expressing MMP-2 and TIMP-2 were vascular endothelial cells. The MMP-2 and TIMP-2 expression was quantita-tively analyzed by image analysis system (HPIAS-1000), and one-way ANOVA(107) and SNK(q) test were done to analyze average absorbance (A) and positive area rate of immunohistochemically positive particles by using SPSS11.5. The results showed: (1) Among 50 samples of haemangioma, there were 26 proliferative haemangiomas, and 24 degenerative haemangiomas, respectively; (2) The expression of MMP-2 was weak in normal vascular endothelial cells, cytoplasm of connective tissues and extracellu-lar matrix around blood vessels. The expression of MMP-2 in proliferative group was significantly higher than in degenerative group and control group (normal skin) (P<0.05), but there was no statisti-cally significant difference between the latter two groups; (3) TIMP-2 was highly expressed in normal tissues, degenerative vascular endothelial cells, cytoplasm of connective tissues and extracellular matrix around blood vessels. The expression level of TIMP-2 in proliferative phase was significantly lower than in degenerative phase (P<0.05), and the expression of TIMP-2 in proliferative phase was signifi-cantly different from that in degenerative phase and normal tissues (P<0.05). It was concluded that in proliferative phase of haemangioma, MMP-2 may promote over-proliferation of endothelial cells of haemangioma, and in degenerative phase, TIMP-2 can inhibit the proliferation of endothelial cells of haemangioma. The two substances play important roles in the genesis, development and degeneration of haemangiomas.
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<p><b>OBJECTIVE</b>To study the changes of neutral alpha-glucoside activity in the epididymis of heroin-dependent and heroin-withdrawal rats, and to investigate the effects of intervention with purine nucleotide (AMP and GMP).</p><p><b>METHODS</b>Eighty Wistar rats were randomly divided into 8 groups of equal number, control, nucleotide, heroin, heroin + nucleotide, 3 d withdrawal, 9 d withdrawal, 3 d nucleotide (nucleotide administrated for 3 days after heroin withdrawal) and 9 d nucleotide (nucleotide administrated for 9 days after heroin withdrawal). Neutral alpha-glucosidase activity in the epididymis was detected in each group of rats.</p><p><b>RESULTS</b>Compared with the control group, neutral alpha-glucoside activity was markedly decreased in the heroin group (P < 0.05), and also in the 3 d and 9 d withdrawal groups, although with no significant differences (P > 0.05).</p><p><b>CONCLUSION</b>Heroin reduces neutral alpha-glucoside activity in the epididymis of rats, and this effect may continue for some time after drug withdrawal, while purine nucleotide can keep neutral alpha-glucosidase activity in a relatively stable state.</p>
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Animals , Male , Rats , Epididymis , Chemistry , Heroin , Heroin Dependence , Metabolism , Purine Nucleotides , Pharmacology , Rats, Wistar , alpha-Glucosidases , MetabolismABSTRACT
Two Rotavirus G9P[8] strains (LL52696 and LL52727) were recognized during a sentinel-based survey in Lulong, China. Phylogenetic analysis of the VP7 gene showed that both strains isolated constituted a divergent genetic cluster distinct from the other G9 strains isolated in China. Analysis of VP4, VP6, and NSP4 genes revealed that these strains were closely related to Lulong strains. We hold that two strains were reassortant between G9 and Lulong predominant strains.
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Humans , Amino Acid Sequence , Antigens, Viral , Chemistry , Genetics , Base Sequence , Capsid Proteins , Chemistry , Genetics , Glycoproteins , Chemistry , Genetics , Phylogeny , Rotavirus , Classification , Genetics , Toxins, Biological , Chemistry , Genetics , Viral Nonstructural Proteins , Chemistry , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To analyze epidemiological characters of an outbreak of rotavirus diarrhea in Daxing County, Guangxi Province.</p><p><b>METHODS</b>Rotavirus-positive specimens were identified by ELISA kit. G/P typing assays were confirmed with multiplex seminested RT-PCR. Full-length VP7 genes of 4 positive specimens were amplified and analyzed.</p><p><b>RESULTS</b>30 cases of Rotavirus-positive were identified from 64 specimens. The attack rate was 46.9%, and G/P typing was G1P[8]. A change of VP7 amino acid residue is at positions 68.</p><p><b>CONCLUSION</b>G1P[8] rotavirus is the etiologic agents of this diarrhea outbreak. In addition, adults were included in this outbreak.</p>
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Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Young Adult , Antigens, Viral , Genetics , Capsid Proteins , Genetics , China , Epidemiology , Diarrhea , Epidemiology , Virology , Disease Outbreaks , Feces , Virology , Genotype , Phylogeny , Rotavirus , Classification , Genetics , Rotavirus Infections , Epidemiology , VirologyABSTRACT
<p><b>OBJECTIVE</b>To explore the efficacy of KGQG and its therapeutic mechanisms in chronic hepatitis B patients with liver fibrosis.</p><p><b>METHODS</b>57 chronic hepatitis B patients with liver fibrosis were randomly divided into two groups: 45 cases in KGQG group were treated by KGQG and routine medications; 12 cases in control group were treated by routine medications only. Serum liver function test results, PCIII, CIV, HA and liver biopsy results of these 57 patients were simultaneously collected and analyzed before and after this intervention.</p><p><b>RESULTS</b>KGQG group showed better efficacy over control group in liver function recovery, decrease of serum PCIII, CIV, HA levels and liver pathologic grades (P 0.05 or 0.01).</p><p><b>CONCLUSION</b>The KGQG could effectively ameliorate liver function and facilitate the inhibition and degradation of liver fibrosis in chronic hepatitis B patients, which may be developed as a novel therapeusis to treat this hard-to-cure disease.</p>
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Adolescent , Adult , Female , Humans , Male , Middle Aged , Collagen Type IV , Drugs, Chinese Herbal , Therapeutic Uses , Hepatitis B, Chronic , Drug Therapy , Pathology , Immunohistochemistry , Liver Cirrhosis , Drug Therapy , Pathology , Medicine, Chinese TraditionalABSTRACT
<p><b>OBJECTIVE</b>To investigate if there is abnormal expression of androgen receptor (AR) in adult diabetic rats.</p><p><b>METHODS</b>The diabetic model rats were induced by using streptozotocin (STZ), which were divided into three groups: control (group C), diabetes (group D) and diabetes with insulin replacement group (group ID). The mRNA and protein expressions of androgen receptor in testis, epididymis and prostate were detected by Northern blot and radioimmunoassay, respectively.</p><p><b>RESULTS</b>Serum testosterone levels and AR mRNA in epididymis of group D or Group ID were lower than those of group C (P <0.05) , respectively, and the protein expression of AR in testis and prostate of group D was lower than that of group C (P <0.05).</p><p><b>CONCLUSION</b>The expression of androgen receptor decreased in testis, epididymis and prostate of diabetic rats, which weakened the biological effects of AR, and it might be one of the causes that resulted in the sexual and productive dysfunction in diabetic rats.</p>
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Animals , Male , Rats , Diabetes Mellitus, Experimental , Metabolism , Epididymis , Metabolism , Prostate , Metabolism , RNA, Messenger , Genetics , Rats, Wistar , Receptors, Androgen , Genetics , Testis , MetabolismABSTRACT
<p><b>OBJECTIVES</b>To study the changes of sexual gland 5 alpha-reductase type II activity in pubertal and adult rats with diabetes.</p><p><b>METHODS</b>We selected 40 and 90 days old male Wistar rats as pubertal and adult animal model respectively, 30 rats in each group. The rats were randomly divided into three groups: control group (C), diabetic group (D) and diabetes with insulin replacement group (ID). The activity of 5 alpha-reductase type II was measured with thin layer chromatography in the epididymis, prostate and testis.</p><p><b>RESULTS</b>1. In all sexual glands of pubertal rats, the activity of 5 alpha-reductase type II in D group is significantly lower than that in C and ID groups. 2. In all sexual glands of adult rats. there is no difference in the activity of 5 alpha-reductase type II among these groups.</p><p><b>CONCLUSIONS</b>The activity of 5 alpha-reductase type II is likely to be influenced by metabolic environment, hormonal levels and local specific factors in pubertal rats, but it is relatively stable in adult rats.</p>
Subject(s)
Animals , Male , Rats , 3-Oxo-5-alpha-Steroid 4-Dehydrogenase , Metabolism , Diabetes Mellitus, Experimental , Epididymis , Prostate , Rats, Wistar , TestisABSTRACT
<p><b>OBJECTIVES</b>To study the effect of antisperm antibody(AsAb) on human sperm acrosin activity.</p><p><b>METHODS</b>AsAb and sperm acrosin activity were measured and analyzed in 3,432 infertile men and 65 fertile volunteers.</p><p><b>RESULTS</b>AsAb positive rate was 10.20% in 3,432 case of male infertility, and 9.37% in 2,882 infertile males who received tests of sperm acrosin activity. Acrosin activity of infertility cases were lower than those of fertile cases(P < 0.001). The comparison between AsAb positive group and AsAb negative group infertility cases showed no significant differences of acrosin activity (P > 0.05). Between normal acrosin activity group and abnormal acrosin activity group, there was no significant difference of AsAb positive rate (P > 0.05).</p><p><b>CONCLUSIONS</b>Antisperm antibody could not affect acrosin activity.</p>
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Adult , Humans , Male , Acrosin , Metabolism , Autoantibodies , Case-Control Studies , Infertility, Male , Allergy and Immunology , Semen , Chemistry , Spermatozoa , Allergy and ImmunologyABSTRACT
In this paper we briefly introduce some microdevices which have been developed recently for biomedical applications by micro-fabrication technology. These applications mainly include areas of diagnostics, drug delivery, tissue engineering and minimally-invasive surgery.