ABSTRACT
Cryptosporidium parvum causes severe gastroenteritis in immunocompromised human and new borne animals. The organism can be transmitted through water. Since small number of C. parvum is infectious, the aim of the present study was to develop a chromatography method for the isolation of C. parvum oocyst in samples with limited number of oocysts. Antibody was prepared against whole antigen from C. parvum oocysts, the achieved Ab bound to the sepharose 4B and used for the isolation of oocysts. Antibody against P23 bound to the sepharose 4B, used also for the isolation of C. parvum oocyst. In comparison to these both methods, 2 traditional methods [Salt floatation and 55% sucrose floatation] were also performed. Both chromatography methods could bind oocysts with capacity depends on the column size. The isolated oocysts were free of bacteria. Our results showed that the traditional methods are useful for the isolation of oocysts from feces, in its smear stained with ziehl-nelsen, at least 3 oocyts are detectable in each microscopic field under 1000 X magnification. In contrast to the chromatography methods, the bacterial contamination was always observed in oocysts isolated with traditional methods. Immunochromatography could be used for the successful isolation of C. parvum oocysts from the samples containing limited number of oocysts
Subject(s)
Animals , Oocysts , Chromatography, AffinityABSTRACT
A 45-year Iranian man referred to the clinic with a severe itching and multiple papular lesions on his trunk, buttocks and extremities. Symptoms and signs of lesions simulated the clinical features of mycosis fungoides [Cutaneous T cell lymphoma]. There were [not any] no typical features of leprosy except a localized patch of numbness on the right hand. Slit skin smear and histopathological slide showed features of lepromatous leprosy. Clinicians practicing in leprosy endemic areas should keep lepromatous leprosy in mind while investigating patients with varied and multiple lesions