ABSTRACT
The distribution of prostaglandin-E2 (PGE2) and prostaglandin-F2alpha (PGF2alpha) was studied in subcellular fractions isolated from homogenates of human atrial fresh tissue by differential centrifugation. Right and left atrial samples were excised from the same heart of six patients with mitral valve disease at the time of open heart surgery. The atrial fractions investigated were mitochondrial (8,500 g pellet), microsomal (100,000 g pellet) and cytosol soluble (100,000 g supernatant) fractions. After extraction of prostaglandins from the three atrial fractions and separation of PGE from PGF series by chromatography on silicic acid column, these prostaglandins were measured by radioimmunoassay. The results, showed that PGE2 and PGF2alpha were located mainly in the soluble cytosolic fraction of right and left atrial tissue (p<0.001). Furthermore, the prostaglandins levels were higher in left than in right atria of these patients (p<0.001). The relation between prostaglandins heart generation in response to elevated work load of mitral valve disease is discussed.
Subject(s)
Humans , Male , Female , Adult , Dinoprostone/metabolism , Dinoprost/metabolism , Mitral Valve Insufficiency/metabolism , Mitral Valve Stenosis/metabolism , Oxytocics/metabolism , Dinoprostone/analysis , Dinoprost/analysis , Heart Atria/chemistry , Heart Atria/metabolism , Mitral Valve/chemistry , Oxytocics/analysis , Subcellular FractionsABSTRACT
Since ovarian sex steroids (estradiol and progesterone) may affect both blood pressure and prostanoids synthesis, and because prostaglandin-E2 (PGE2) and prostacyclin (PGI2) can modulate the vascular action of pressor hormones, we investigated the vascular reactivity to norepinephrine during the estrous cycle of the rat. In addition, we determined the vascular biosynthesis of PGE2 and 6-keto-PGF1 alpha (the stable metabolite of PGI2) at different stages of the estrous cycle. Cumulative dose-response curves were obtained by a stepwise increase in the concentration of norepinephrine. The contraction of thoracic aortic rings induced by norepinephrine did not change significantly between estrus, metestrus and diestrus. However, aortic rings obtained on proestrus showed a significant reduction in the maximal contraction (Emax) induced by norepinephrine (p < 0.001). In addition, we found significant increases in vascular synthesis of PGE2 and PGI2 on proestrus (p < 0.001). These results indicate that vascular reactivity and vascular prostanoids synthesis are influenced by the hormonal changes occurring during the estrous cycle of normal female rats. It is possible that prostanoids generated locally may play an important role in the regulation of vasomotor tone in the systemic vascular bed throughout the estrous cycle
Subject(s)
Animals , Female , Rats , Aorta, Thoracic/physiology , Estrus/physiology , Norepinephrine/pharmacology , Prostaglandins/biosynthesis , Blood Pressure/drug effects , Dinoprostone/biosynthesis , Dose-Response Relationship, Drug , Epoprostenol/biosynthesis , Rats, Sprague-Dawley , Steroids/physiologyABSTRACT
To assess whether prostaglandin-E2 (PGE2) and cyclic adenosine 3'-5'-monophosphate (cAMP) are involved in the cardiac response to chronic pressure overload, we measured by specific radioimmunoassay method the cardiac tissue and plasma concentrations of PGE2 and cAMP in an animal model of left ventricular hypertrophy. The cardiac hypertrophy was accompanied by a significant increase in PGE2 content, and a significant decrease in cAMP content, in the heart. In addition, we found elevated PGE2 and cAMP levels in arterial plasma samples from the rats with hypertrophied hearts compared to normal rats. These findings suggest a link between cardiac and vascular PGE2 and cAMP generation and the hemodynamic stresses of advanced cardiac overload