ABSTRACT
The Chronic toxicity study of Nigella saliva seeds [Black seeds] oil has been evaluated in male and female rats. The Nigella saliva seeds oil was administered following a dose of 2.5 ml and 5 ml for 12 weeks by oral administration. All animals tolerate N. sativa oil during experiment and show no sign of toxicity or discomfort with any significant changes in clinical signs This oil produces no significant changes in hematological indices [hemoglobin [Hb], Packed Cell Volume [PCV], Mean Corpuscular Hemoglobin [MCH], Mean Corpuscular Hemoglobin Concentration [MCHC], Mean Corpuscular Volume [MCV], Red Blood Cell Count [RBC], and White Blood Cell Count [WBC] following the oral administration. Results taken as whole indicate that Nigella saliva seed oil is non-toxic for rats in long-term use. The sign and symptoms exhibited by chronic studies were almost alike and conform it non toxic up to wide range of dose