Sensitivity of Jatropha curcas seeds to (60)Co-gamma radiation and their medial lethal doses in radiation breeding / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the sensitivity of Jatropha curcas seeds from three different locations to (60)Co-gamma radiation and to determine the medial lethal doses (LD50) of (60)Co-gamma radiation for these seeds.</p><p><b>METHODS</b>Six different radiation doses (0, 100, 150, 200, 250 and 300 Gy) were used. Based on the germination rate 50%, LD50 doses of (60)Co-gamma radiation for the seeds were calculated using linear regression equation.</p><p><b>RESULTS</b>LD50 doses of (60)Co-gamma radiation for these seeds were 178 Gy (seeds from Guangdong), 132 Gy (seeds from Hainan) and 198 Gy (seeds from India) respectively. Increasing radiation doses caused more significant changes in leaf shape of the M1 seedlings.</p><p><b>CONCLUSION</b>The results provides an important experimental basis for the radiation breeding of the important herbal and energy plant J. curcas.</p>

Oil contents and fatty acid composition in Jatropha curcas seeds collected from different regions / 南方医科大学学报

<p><b>OBJECTIVE</b>To compare the oil contents and fatty acid composition among the samples of Jatropha curcas L. seeds collected from China (Guangdong, Hainan, and Guizhou Provinces) and India.</p><p><b>METHODS</b>Soxhlet extraction method and gas chromatography-mass spectrometry (GC-MS) were employed to determine the oil contents of Jatropha seeds and the fatty acid composition of Jatropha oil.</p><p><b>RESULTS</b>The seed oil contents (dry basis) were 32.43% (Guangdong), 31.41% (Hainan), 37.56% (Guizhou) and 41.04% (India), respectively. Twelve different fatty acids were detected by GC-MS, and the content of total unsaturated fatty acids accounted for 80.93%, 79.53%, 77.24% and 78.22% of the total fatty acids in the samples collected from Guangdong, Hainan, Guizhou and India, respectively.</p><p><b>CONCLUSION</b>There are differences in the oil contents and fatty acid composition among the J. curcas seeds collected from different regions, and attention should be given to these differences in the introduction and breeding of J. curcas.</p>

Efficient and rapid non-test tube cloning of Jatropha curcas / 南方医科大学学报

<p><b>OBJECTIVE</b>To develop a new technique for efficient and rapid non-test tube cloning of the medicinal and energy- producing plant Jatropha curcas.</p><p><b>METHODS</b>Using the mini-stem fragment (2-3 cm) of Jatropha curcas with merely one axillary bud as the explant, the effect of an auxin IBA concentration on the plantlet regeneration was studied.</p><p><b>RESULTS AND CONCLUSION</b>When treated with 1 mg/LIBA for 1h, the explants showed the most rapid propagation. The mini-stem fragments high root regeneration ratio (96.7%), short root regeneration period (18.2-/+2.0 d), large number of new roots per explant (6.3-/+1.8), and long total root length (6.8-/+3.5 cm), demonstrating that this technique can be a simple and efficient method for rapid non-test tube cloning of Jatropha curcas of potential industrial value.</p>