ABSTRACT
AIM: To study the change of blood lipid and difference of expressed gene in chest aorta cells of atherosclerosis model rats with or without the treatment of Taishan Ganoderma Lucidum amylose.METHODS: The concentrations of triglyceride (TG), total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) in serum were measured by automatic serum biochemical assay. Differential-display reverse transcription-PCR (DDRT-PCR) was used to analyze the differential expressed gene in chest aorta cells of atherosclerosis model rat with or without the treatment of Taishan Ganoderma Lucidum amylose. The differential expressed cDNA was confirmed by reverse Northern blotting.RESULTS: Up-regulated and down-regulated cDNA were isolated in chest aorta cells of atherosclerosis model rats with or without Taishan Ganoderma Lucidum amylose treatment. The known up-regulated genes included cytB561, ERP72, nitric oxide synthase 2 and Gpx1. The known down-regulated genes included thymosin β4, FGFRI oncogene, ICAM-1, FK506 binding protein, c-myc and TNF-α. CONCLUSION: Taishan Ganoderma Lucidum amylose decreases the level of blood lipid.
ABSTRACT
Pim-1 belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Originally identified from moloney murine leukemia virus (MuLV)-induced T-cell lymphomas in mice, Pim-1 kinase is involved in the control of cell growth, differentiation and apoptosis. Expression of Pim-1 kinase can be stimulated by a variety of growth factors and regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Several signal transduction pathways may be associated with the regulation of Pim-1's expression; accumulating data support that the expression of Pim-1 protein is mediated through activation of JAK/STATs. Recent studies of Pim family kinases indicate that Pim-1 kinase plays important roles outside of the hematopoietic system as well.