ABSTRACT
AIM Research on effective dialysis medicine through partial isolation loop of small intestine to treat renal failure. METHODS Divide 10 dogs equally into group A and group B for comparative study: make full isolation loop of small intestine; input hypersonic sample solution through vein after blocking the renal arteries to make the man made real failure models such as electrolytic disorder, azotemia and so on. Then at 0 5 hour and 5 hours after that, inject 0 9% NaCl 0 5 ml for group A and 0 025 mg?kg -1 of neostigmine for group B. Finally collect the secretion fluid of the small intestine loop and blood samples every 30 minutes from group A and group B respectively so as to determine the density of K + ,Na +, Cl -,UN, CR,UA in the small intestine fluid and blood samples of the two groups and calculate the clearance rate of each group. RESULTS Respectively at 1 5 hours and 6 5 hours after being injected with neostigmine, group B got two secretion peaks and its clearance rate is remarkably higher than that of group A( P
ABSTRACT
Objective:To explore the possible role of ? endorp hi n in effect of melatonin on morphine withdrawal symptoms in rats.Method:Acute model of morphine dependent rat was estalished by gradually increasing the dose of morphine through subcutaneous injections.CSF(cerebrospinal fluid)w a s got by push-pull perfusion and intracerebroventricular injection(icv)in a fixe d tuber.Concentration of ? endorphin in CSF was determined by radioimmunoassa y (RIA).Results:(1)The symptoms of natural morphine withdrawal were r e lieved significantly by icv ? Endorphin with a dose dependent manner.(2)The s y mptoms of natural morphine withdrawal were also relieved by ip melatonin.(3)The CSF ? endorphin level was significantly lower in morphine dependent rats than normal control,but that was higher in natural morphine withdrawal rats.That was also much more increased by ip melatonin in the withdrawal rats than in morphine dependent rats or normal control.Conclusion:The withdrawal symptom s could be relieved by icv ? endorphin or by ip melatonin in morphine dependetn rats.The mechanism may be partly due to the elevation of endogenous ? endorp hin level in brain of rats in withdrawal