ABSTRACT
Objective: To prepare morin-Cu2+ complex imprinted polymer (CIP) and use the polymer to extract morin from mulberry branch. Methods: Morin-Cu2+ CIPs were prepared in water-methanol polar solvent in the presence of Cu2+, acrylamide as the functional monomer. The CIPs were used as the solid phase extraction sorbent for extracting morin from mulberry branch. Results: The prepared CIPs had specific and selective adsorption on morin, the maximum adsorption capacity was 82 μmol/g, which was much higher than that of the traditional imprinted polymers relying on hydrogen bond and non-imprinted polymers. The separation factors of CIPs to daidzein and catechin were 4.81 and 4.02, respectively. CIPs had a significant enrichment effect on morin in the composition of the mulberry branch solid phase extraction elution solution. Conclusion: Morin-Cu2+ CIPs are excellent material used for the separation and enrichment of morin, the material has good selective adsorption ability and environmental adaptability.