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Chinese Journal of Virology ; (6): 58-64, 2010.
Article in Chinese | WPRIM | ID: wpr-297917

ABSTRACT

The plaque-forming characteristics of Newcastle disease viruses of chickens and geese source were compared on various cells. The result showed that there were obvious differences of plaque formation between F48E9 and NA-1 on Vero cells, chicken embryo fibroblast cells (CEF) and goose embryo fibroblast cells (GEF). The plaque-forming ability of NA-1 was higher than F48E9 on GEF, but lower than F48E9 on CEF. On Vero cells, the plaque-forming ability of NA-1 was slightly stronger than F48E9. It demonstrated that the plaque-forming characteristics were consistent with host tropism of virus. The morphogenesis of F48E9 and NA-1 on Vero cells was observed with transmission electron microscope. There were different replication processes between F48E9 and NA-1 on cells at different stages. NA-1 had stronger adaptability to host than F48E9 according to budding processes and envelope integrity.


Subject(s)
Animals , Chick Embryo , Chlorocebus aethiops , Chickens , Geese , Host-Pathogen Interactions , Newcastle Disease , Virology , Newcastle disease virus , Physiology , Poultry Diseases , Virology , Vero Cells , Viral Plaque Assay
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