ABSTRACT
Objective:To investigate the effects of Ligustrazine (TMP) on the expression of caspase-3 and ABR threshold in guinea pig cochlea after gentamicin (GM) ototoxicity. Methods:A total of 80 guinea pigs were randomly divided into saline group, GM group, GM+TMP group and TMP group. The duration of treatment was lasted 10 d continuously in all groups. The dose was decided by their weight. The ABR threshold were measured before and after administration of 10 d. SABC immunohistochemical method was used to detect the expression of caspase-3 in the spiral ganglion,cochlea hair cells,and stria vascularis of guinea pigs. Results:(1) The average gray value in the spiral ganglion cells, stria vascularis and hair cells in GM group had significant difference with those in the same position of control group (P<0.05) .The average gray value in the spiral ganglion cells and hair cells in GM+TMP group had no difference with those in the same position of control group (P>0.05), but in stria vascularis (P<0.05) . Meanwhile the average gray value in spiral ganglion, hair cells, stria vascularis in TMP+GM group had significant difference with those in GM group (P<0.05) . (2) The ABR threshold had no significant differences before treatment (P>0.05) . After administration of 10 d, the ABR threshold of GM group was significantly changed (P<0.05) . The ABR thresholds in GM group was significantly higher than that in control group (P<0.05) . The ABR threshold in TMP+GM group was lower than that in GM group (P<0.05) . Conclusion:TMP participates in preventing the process of cochlea injury caused by GM,through blocking the expression of caspase-3 in cochlea tissue of GM ototoxicosis probably,which achieve the protection of GM deafness eventually.
ABSTRACT
Objective to explore the safety and clinical efficacy of CT-guided radioactive seed implantation in treating recurrent rectum carcinoma. Methods CT-guided ~(125)I radioactive seed implantation was carried out in 20 patients with recurrent rectal carcinoma. Treatment planning system was used preoperatively to reconstruct three dimensional image of the tumor and to calculate the estimated seed number and distribution. The tumor matched peripheral dose (MPD) of the radioactive seeds was 80-130 Gy. The radioactivity of the seeds was 0.5-0.8 mCi/seed and the median implanted seeds was 48 (range 25-95) in number. CT scan was made immediately after the implantation to check the quality of the seeds. Change of pain score, tumor size and complications were recorded during the follow-up period. Results Twenty cases composed of 12 males and 8 females, aged 38 to 78 years (median age of 62 years). The follow-up period lasted 2-28 months. On an average, 3 to 7 days after the procedure patients experienced significant pain relief. CT scan performed 2 months after the procedure revealed that complete relief (CR) of the tumor was seen in 2 cases, partial relief (PR) in 13 cases, no change (NC) in 3 cases and progression (PD) in 2 cases. The total effective rate (CR + PR) was 75%. The median survival time was 18.8 months. The survival rate of 1 and 2 years was 75% and 25% respectively. Two cases died of tumor deterioration and 3 cases died of extensive metastases. No complications such as frequent micturation, pain on urination and hematuria occurred during the follow-up period. Conclusion CT-guided ~(125)I radioactive seed implantation is a safe and effective interventional treatment for recurrent rectal carcinoma with reliable short-term efficacy and excellent anti-pain effect.