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Objective:To explore the value of uterine volume, ovarian volume (OV), endometrial thickness (ET) combined with ovarian hemodynamics in polycystic ovarian syndrome (PCOS).Methods:Fifty-three patients with PCOS diagnosed in the Third Affiliated Hospital of Wenzhou Medical University from October 2017 to October 2019 were selected as observation group and 53 healthy women of child bearing age who underwent physical examination at the same time were selected as control group. Patients with PCOS were divided into groups according to their different menopause times and ovalution statusafter ovulation promotion. The uterine volume, OV, ET, peak ovarian systolic velocity (PSV), ovarian artery resistance index (RI), and ovarian artery pulsation index (PI) was measured and recorded by transvaginal color Doppler ultrasound and compared between observation group and control group; and the above indicators in PCOS patients before and after chorionic gonadotrophin injection and 7 d after ovulation were compared.Results:Before treatment, the value of uterine volume, RI and PI of the observation group were lower than those of the control group: (30.28 ± 11.37) cm 3 vs. (38.41 ± 13.02) cm 3, 0.31 ± 0.04 vs. 0.73 ± 0.09, 0.86 ± 0.24 vs. 1.16 ± 0.32, the OV, ET, and PSV were higher than those of the control group: (15.04 ± 3.06) cm 3 vs. (6.35 ± 1.32) cm 3, (1.32 ± 0.41) mm vs. (0.64 ± 0.16) mm, (12.27 ± 2.83) cm/s vs. (9.37 ± 1.53) cm/s, and the differences were statistically significant ( P<0.05). Before treatment, the uterine volume and OV of PCOS patients had no significant differences in different menopause groups ( P>0.05). In menopause 10 - 15 d group, menopause 16 - 30 d group, menopause 31 -90 d group, and menopause>90 d group, the ET and PSV were gradually higher, the RI and PI were gradually lower, and the differences were statistically significant ( P<0.05). After ovulation promotion among 53 PCOS patients, 48 patients succeeded in ovulation promotion and 5 patients failed. On the day of chorionic gonadotrophin injection and mid luteal phase,the OV, ET and PSV in the successful ovulation induction group were lower than those in the failed ovulation induction group, and the uterine volume, RI and PI were higher than those in the failed ovulation induction group, and the differences were statistically significant ( P<0.05). In the successful ovulation induction group, the ET, RI, PI onthe day of chorionic gonadotrophin injection were lower than those onmid luteal phase, PSV was higher than that on mid luteal phase, and the differences were statistically significant ( P<0.05). Receiver operating characteristic (ROC)curveanalysis showed that that the area under the curve(AUC) of the value of uterine volume, OV, ET, and ovarian hemodynamic indicators joint examinationfor PCOS was 0.897 (95% CI 0.795 - 0.968), with a sensitivity of 95.0%, and a specificity of 80.2%, and all were higher than that of the single examination ( P<0.05). Conclusions:The value of uterine volume, OV, ET combined with ovarian hemodynamics are beneficial to observe the ovarian function of PCOS patients and the developmental potential of the basal layer of the endometrium, and have good application value for the evaluation of PCOS patients and guidance of clinical diagnosis and treatment.
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Six years' English teaching in department of obstetrics and gynecology in Wenzhou Medical College were reviewed. Language and cultural differences are the main reasons hindering teaching quality. Rational use of a variety of teachers,preparing for lessons adequately,adoption of English image data,supplying and revising English teaching materials,using network auxiliary teaching and forming ef-fective education mode are conducive to improving English teaching quality in department of obstetrics and gynecology for foreign students.
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Objective To investigate the expression and clinical significance of lipoxin A4,leukotrienc C4,lipoxygenase-5 in peripheral blood of pregnant women with different types of severe preeclampsia.Methods Forty-five singleton pregnant women who accepted antenatal care and delivered in First Affiliated Hospital of Wenzhou Medical College were enrolled in this study from December 2010 to June 2011.All objects were divided into normal pregnancy group (n=20),early onset severe preeclampsia group (n=10) and late onset severe preeclampsia group (n=15).Enzymelinked immunosorbent assay was used to detect lipoxin A4 and leukotriene C4 levels in peripheral blood.The level of lipoxygenase-5 mRNA in white blood cells was detected by real time fluorescence quantitative reverse transcription-polymerase chain reaction.The differences of lipoxin A4,leukotriene C4 and lipoxygenase-5 mRNA among groups were compared by analysis of variance and LSD-t test;and correlations among their expressions were analyzed by linear regression.Results Lipoxin A4 level in early and late onset severe preeclampsia group was (355.3±116.0) pg/ml and (389.7±117.5) pg/ml,which were both significantly lower than that in normal pregnancy group [(555.0±139.8) pg/ml] (t=-4.03 and-3.77,P＜0.05 respectively).The leukotriene C4 level in early and lateonset severe preeclampsia and normal pregnaney group was (591.3±185.5) pg/ml,(510.3±197.1) pg/ml and (496.9 ± 158.8) pg/ml,no statistical difference were found (F=0.889,P＞0.05) ; neither did the expression of lipoxygenase 5 mRNA,which was 4.2± 1.9 in normal pregnancy group,4.8 ± 2.0 in early onset severe preeclampsia group and 4.4 ± 1.2 in late onset severe preeclampsia group (F=0.311,P＞0.05).There was no correlation among the levels of lipoxin A4,leukotriene C4 and lipoxygenase-5 mRNA in each group (P ＞ 0.05).Conclusions Early and remarkable decreasing of lipoxin A4 level might contribute to the development of early onset severe preeclampsia.
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ObjectiveTo investigate the effects of 5(S),6(R),7-trihydroxyheptanoic acid methyl ester (BML-111) on pregnant mice with fetal growth restriction(FGR) induced by antenatal dexamethasone and its probable mechanism. MethodsThe mice were mated overnight,with day 1 of pregnancy designated as the day on which spermatozoa were presented in a vaginal smear.The pregnant mice were then randomly divided into control group,dexamethasone group and BML-111 group.From 9 to 14 days of pregnancy,pregnant ICR mice of control,dexamethasone and BML-111 group were treated separately with saline,dexamethasone(5 mg/kg) and dexamethasone at 8:00 am,and two hours later they were treated separately again with 1 mg/kg saline,saline and BML-111.On the day 18 of gestation,they were sacrificed after blood were collected from their eyeballs.The serum lipoxin A4 was measured with enzyme-linked immunosorbent assay. Fetuses were delivered by cesarean section; the placenta and uterus were immediately removed and frozen.Gene expressions of 11β-hydroxysteroid dehydrogenase 2 ( 11β-HSD2 ),interleukin-1β (IL-1β) in placenta and lipoxin A4 receptor-formyl peptide receptor 3 (FPR3)in uterine were detected by reverse transcriptionpolymerase chain reaction and compared with analysis of variance.The 11β-HSD2 protein in mice placenta was detected by immunohistochemistry. ResultsThe mean fetal weight of dexamethasone group was (0.823±0.054) g,lower than that of the control group and BML-111 group [(1.103±0.218) g and (0.992 ± 0.207) g] (t =- 4.108 and - 2.890,P ＜ 0.05 respectively).Protein expression of 11β-HSD2 in dexamethasone group (0.030±0.019) was weaker than that in control group (0.058±0.015,t=-3.107,P＜0.05) or in BML-111 group (0.049±0.026,t=-2.211,P＜0.05).The expression of 11β-HSD2 mRNA in dexamethasone group (0.457±0.062) was lower than that in control group (0.943±0.057,t=-9.418,P＜0.05) or in BML-111 group (0.698±0.071,t=-4.617,P＜0.05).Expression of IL-1β mRNA in dexamethasone group (0.543±0.103)was less than that in control group (0.710± 0.085,t=-3.736,P＜0.05) but more than that in BML-111 group (0.229 ±0.031,t=7.025,P＜0.05). The expression of FPR3 mRNA in dexamethasone group (0.323 ± 0.019) was less than that in control group (0.857 ± 0.057,t =-14.630,P＜0.05) or in BML-111 group (0.499 ±0.050,t=-4.822,P＜0.05).The serum concentration of lipoxin A4 in dexamethasone group was lower than that in control group [(64.463±22.144) pg/ml vs (101.610±24.916) pg/ml,t=3.152,P＜0.05].ConclusionsBML-111 regulate the expression of 11β-HSD2 and then protect against FGR resulted from too much prenatal application of dexamethasone.
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OBJECTIVE To evaluate the clinical application of flow-through hybridization to human papillomavirus(HPV) subtype detection.METHODS A total of 305 female patients infected with HPV were selected for HPV subtype analysis using flow-through hybridization and the assessment of HPV subtype on cervical lesions was analyzed.RESULTS All 21 different subtypes were found.From 305 cases 173 patients were infected with single-type HPV and 132 patients were infected with multiple-type HPV.The most commonly found high-risk types were HPV16(17.6%),HPV52(9.8%),HPV58(9.0%),HPV33(6.3%),HPV18(5.4%) and HPV68(5.2%) and the low-risk types were HPV11(14.3%) and HPV6(6.5%).The high-risk HPV increased and the low-risk HPV declined along with the upgrade of the cervical lesions.CONCLUSIONS Technology of flow-through hybridization is able to detect multiple HPV subtype.The distinction between low and high-risk HPV subtypes is seemed useful in prevention and management of cervical cancer.