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Objective:To explore the mechanism of grape seed polyphenols (GSP) reverseing mutidrug resistance of GBC-SD cell lines.Methods:GBC-SD cell lines were used to determine the effect of GSP.MTT assay was adopted to evaluate the cytotoxity,RT-PCR was used to determine MDR1 mRNA,P-gp and cellular adriamycin was measured by flow cytometry.Results:IC50 of chemotherapeutic agents was reduced in GSP treated group (P0.05),GSP inhibited the expression of P-gh and MDR1 mRNA (P0.05) ,increased celluar accumulation of adriamycin (P0.05).Conclusions:GSP can reverse mutidrug resistance of GBC-SD cell lines,the potential mechanisms is that GSP decreases expression of P-gp、bcl-2 and MDR1 mRNA.
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Objective:Study on the expression of Fas and Fas ligand (FasL) in gastric cancer and its possible significance.Methods:Fifty-eight paraffin-embedded gastric cancer tissues and twenty-six non-cancer tissues were tested for the expression of Fas and FasL protein by immunohistochemistry.Results:The positive rate of Fas in cancer cells of gastric cancer tissues was significantly lower than that in gastric epithelial cells of the control tissues(19.0% and 61.5%,respectively;?~2=14.918;P=0.000).The positive rate of FasL showed no significant difference between cancer cells of gastric cancer tissues and gastric epithelial cells of the control tissues(63.8% and 53.8%,respectively).Conclusion:The Fas-FasL system is unbalanced.It may be related to the carcinogenesis of gastric epithelial cells and might be responsible for the immune excape of these cells.
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Objective: To study the expression of Fas, Fas ligand (FasL) and IFN ? in gastric cancer and its possible significance. Methods: Fifty eight gastric paraffin wax embedded cancer tissues and fifty three normal tissues adjacent gastric cancer were tested for the expression of Fas and FasL protein by immunohistochemistry and IFN ? mRNA by in situ hubridisation respectively. Results: The positive rate of Fas in cancer cells of gastric cancer tissues was significantly lower than that in gastric epithelial cells of the tissues adjacent cancer(19.0% and 64.2%, respectively; ? 2=23.46, P = 0.00). The positive rate of FasL in cancer cells of gastric cancer tissues was significantly higher than that in gastric epithelial cells of the tissues adjacent cancer(63.8% and 45.3%,respectively; ? 2=3.83, P =0.05). The positive rate of IFN ? in cancer cells of gastric cancer tissues was significantly lower than that in gastric epithelial cells of the tissues adjacent cancer(0.0% and 49.1%,respectively; ? 2=37.16, P =0.00). Conclusion: The Fas-FasL system was unbalanced, and the expression of IFN ? was low in gastric cancer cells in this study. These may be related to the carcinogenesis of gastric epithelial cells and might be responsible for the immune escape of these cells.
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<p><b>OBJECTIVE</b>To establish a new extrahepatic growing hepatocellular carcinoma cell line.</p><p><b>METHODS</b>A specimen from extrahepatic growing hepatocellular carcinoma was cultured in vitro. Cancer cells were studied morphologically and subjected to karyotype analysis, DNA analysis, and tumor formation evaluation.</p><p><b>RESULTS</b>Morphological observation and functional analysis showed that their features were similar to those of HCC. Chromosomes with a variation of 76 approximately 104 were able to secret AFP in vitro and to form bile canaliculi with microvilli.</p><p><b>CONCLUSION</b>EGHC-9901 cell line has characteristics of the extrahepatic growing hepatocellular carcinoma.</p>
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Adult , Animals , Humans , Male , Mice , Carcinoma, Hepatocellular , Genetics , Pathology , Chromosome Aberrations , Liver Neoplasms , Genetics , Pathology , Mice, Nude , Tumor Cells, Cultured , alpha-FetoproteinsABSTRACT
Objective To study the etiology,diagnosis and treatment of functional delayed gastric emptying(FDGE) resulting from pancreatoduodenectomy.Methods From June,1990~June,2003,136 patients received pancreatoduodenectomy,whose clinical data were retrospectively analyzed.Upper gastrointestinal(radiography) and endoscopy were the main methods of examination.Results Twenty-eight cases were(complicated) with FDGE in the 136 patients(20.6%) after operation.The occurrence of FDGE was(correlated) with hyperbilirubinemia,diabetes,duodenal obstruction,pancreatic fistula and abdominal infection.All patients were cured with conservative treatment.The recovery time of gastric motility was 14-42 days,average time was 28 days.Conclusions Hyperbilirubinemia,diabetes,duodenal obstruction,pancreatic(fistula) and abdominal infection were the main causes of FDGE.
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Objective: To study the effect of hyperthermia treatment on apoptosis of human gallbladder carcinoma cell line GBC-SD.Methods: Human cultured gallbladder carcinoma cell GBC-SD in vitro was heated for 1 hour at 40℃ and 43℃.Then 6h、12h、20h later GBC-SD cells were collected,respectively.The morphologic character was observed under the microscope,the biological character was ascertained by DNA electrophoresis,the percentage of the cell apoptosis and the alteration of cell cycle were detected by flow cytometry.Results: After 1 hour heated at 43℃,gallbladder carcinoma GBC-SD cells occurred the most obvious apoptosis in 12h and 20h.Typical apoptotic bodies appeared,and DNA Ladder was demonstrated on DNA electrophoresis.Apoptotic peak and the change of cell cycle were tested by the flow cytometry.Conclusion:One hour moderate hyperthermia treatment at 43℃ is the effective heat-dosage to induce human gallbladder carcinoma GBC-SD cell apoptosis. [
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To establish a new extrahepatic growing hepatocellular carclnoma cell line. Methods: A speciment that was pathologically identified extrahepatic growing hepatocellular carcinoma were cultured in vitro. The morphology,karyotype analysis, DNA analysis, the tumor formation of heterotransplantation were observed. Results: Morphological observation and functional analysis showed that it had the comnnon features of HOC.It was found to be able to secret AFP in vitro. Conclusion: EGHC-9901 has characteristic of the extrahepatic growing hepatocellular carcinoma cell line.
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0.05) was observed. Bcl 2 expression was related with differentiation of GC(P0.05).Conclusion: p53,bcl 2,c erbB 2 may be involved in the development of GC.
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Objective:To investigate the different killing effects on human hilar cholangiocarinoma cells FRH with cytosine deaminase(CD) and herpes simplex virus thymidine kinase(HSV tk)double suicide genes coexpression compared with single gene mediated by retrovirus.To find a more efficient and low toxicity suicide gene therapy for hilar cholangiocarinoma.Methods:CD and HSV tk double suicide genes were transfected into PA317 cells using lipofectamine.The positive clones were picked out and cultured after G418 selected.The viral supernatant was collected.The FRH cells were infected with the virus containing the double suicide genes.After G418 selection,RT PCR was resorted to demonstrated the successful transcription of CD and HSV tk genes.The FRH/CD+tk and FRH cells in culture were respectively treated with 5 Fc and /or GCV.The cytoxicity efficacy was evaluated by microculture tetrajolium test (MTT) method.Results:The virus containing double suicide gene was produced in PA317 cells.Double suicide genes were stably expressed in RFH cells after being infected with the virus.The killing effect of combination 5 Fc with GCV on FRH/CD+tk cells is more effective than that of using 5 Fc or GCV alone.Conclusion:The CD+TK/5 Fc+GCV co expression system is more effective for killing effects on FRH cells than that of CD/5 Fc or tk/GCV system alone.
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Objective: To investigate effect of exogenous wild-type p53 gene on the cell growth and tumorigenicity of human gallbladder cancer cell lines. Methods: After identification of the genetic status of p53 gene of GBC-SD cell lines with the immunocytochemistry staining and the direct sequencing technique of PCR products, eukaryotic expressing plasmid pCMV-p53 was introduced by lipofectamine-mediated into GBC-SD cell lines. Growing transfected cells were selected by G418. The presence and expression of exogenous p53 gene was detected by PCR, RT-PCR and Western blot. The cellular proliferating ability was assessed using the cell growth curve and cloning assay. The xenograft in nude mice was performed to examine the effect of tumorigenicity. Results: P53 protein overexpression was showed in GBC-SD cell lines. A transversion of TAC→AAC at codon 126 of exon 5 was confirmed. PCR, RT-PCR and Western blot showed exogenous p53 gene had successfully transfected into GBC-SD cells and obtained high expression. The growth and proliferation of the cells were greatly decreased, and the tumorigenicity was significantly inhibited after transfection wtp53. Conclusion: The expression of exogenous wild-type p53 gene could effectively inhibit the growth of gallbladder cancer GBC-SD cells in vitro and in vivo.
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Objective To study the X chromosome aberration in hepatocellular carcinoma cell line HCC-9903. Method The hepatocellular carc inoma cell line, HCC-9903, was detected by fluorescence in situ hybridization ( FISH) centromere DNA probes of X chromosome. Result Increased X chromosome copy number was observed in HC C-9903 cell line , four signals of hybridization was the major type. Conclusion Gain of X chromosome freq uently occurred in liver carcinoma. The relationship between gain of X chromosom e and liver carcinoma genesis needs further investigation.
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Objective To explore the mechanism of reversal of mutidrug resistance of GBC-SD cell lines by grape seed polyphenols(GSP).Methods GBC-SD cell lines were used to determine the effect of GSP.MTT assay was adopted to evaluate the cytotoxity(IC_(50)),RT-PCR were used to determine MDR1mRNA,(P-gp),bcl-2 and cellular adriamycin was measured by flow cytometry.Results In non-toxic(3?g/mL) and low toxic(6?g/mL) comcentration of GSP treated group(P