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Objective To explore the association between serum FGF23 and Klotho protein,and bone mineral density in maintenance hemodialysis (MHD) patients.Methods A total of 125 MHD patients admitted in the Hospital between January 2015 and November 2015 was enrolled.Their bone mineral densities of femur neck and lumbar spine were studied by dual-energy X-ray absorptiometry.These patients were divided into three groups as normal,osteopenic and osteoporotic,according to World Health Organization criteria based on bone mineral density T scores.Levels of serum FGF23,Klotho protein and 1,25(OH)2VitD3 were measured by ELISA.The parameters including calcium,phosphorus,and parathyroid hormone were assessed.Results The incidences of osteopenia and osteoporosis at the femur neck and lumbar spine in MHD patients were 82.40％ and 56.00％ respectively.No significant difference was found in the levels of serum FGF23 among normal,osteopenic and osteoporotic groups on the basis of femur neck and lumbar spine bone mineral density (P ＞ 0.05).No correlation was found between FGF23 and bone mineral density.There however were significant differences in the levels of serum Klotho protein among three groups on the basis of femoral neck bone mineral density (P ＜ 0.05).And the levels of Klotho protein in the osteoporotic group [(387.172±54.137) ng/L] were significantly decreased than those in normal group [(429.883±41.776)ng/L] and osteopenic group [(410.598±61.056) ng/L] (P ＜ 0.05).There were also significant differences in the levels of serum Klotho protein among three groups in terms of lumbar spine bone mineral density (P ＜ 0.05),while the levels of Klotho protein in the osteopenic group [(387.263 ± 53.255) ng/L] were significantly decreased than those in normal group [(417.108±56.179) ng/L] (P＜ 0.05).A positive correlation was found between Klotho protein and bone mineral densities of femur neck and lumbar spine.Multiple linear regression analysis showed that one of the main factors influencing the degree of bone mineral density in MHD patients was Klotho protein.Conclusions CKD-MBD with low BMD is common and widespread in hemodialysis patients.FGF23 has no direct effect on bone mineral density in MHD patients;while Klotho protein is correlated with the severity of bone mineral density.High-level Klotho protein may reduce the severity of CKD-MBD with low BMD in MHD patients.
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Objective To investigate the factors correlated to coronary artery calcification (CAC) in maintenance hemodialysis (MHD) patients.Methods This study included 132 patients(54 females,78 males),aged 26-94 years,who were on hemodialysis for 10-204 months(median dialysis duration 51.00 months).The parameters including calcium,phosphorus,parathyroid hormone,total cholesterol,low density lipoprotein,triglycerides,C-reactive protein (CRP),klotho,and so on were assessed.Quantification of CAC was determined by multi-slice spiral computed tomography (MSCT),known as the coronary artery calcification score (CACs).Results Ninety-two patients (69.70％) had CAC,with CACs ranging from 0 to 13 450.20.More than 30％ patients experienced one even a variety of cardiovascular and cerebrovascular diseases.A positive correlation was observed between the degree of CAC and the incidence of cardiovascular and cerebrovascular diseases.Whereas a positive correlation existed between CACs and age (r=0.347,P=0.000),duration of hemodialysis (r=0.245,P=0.005),systolic blood pressure (r=0.184,P=0.034),diabetes history (r=0.211,P=0.015),phosphorus (r=0.262,P=0.002),calcium-phosphorus product (r=0.247,P=0.004);and a negative correlation between CACs and klotho level (r=-0.294,P=0.001).Multivariate logistic regression analysis showed that the main factor influencing the degree of CAC in MHD patients was age.Conclusions CAC is common and widespread in hemodialysis patients,who are often accompanied by cardiovascular and cerebrovascular diseases.The prevalence rate of cardiovascular and cerebrovascular diseases increases with the aggravation of CAC degree.Age,duration of hemodialysis,systolic blood pressure,diabetes history,disturbance of calcium and phosphorus metabolism and klotho are correlated with the severity of CAC.Age is an independent risk factor of CAC degree.
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Objective To examine the correlation of serum neutrophil gelatinase-associated lipocalin (NGAL) with dialysis adequacy,microinflammatory state and iron metabolism,and to assess the value of NGAL in identifying the dialysis adequacy in maintenance hemodialysis (MHD) patients.Methods A total of 150 MHD patients and 50 healthy control people were enrolled in the study.Clinical data were collected and serum NGAL,CRP,transferrin saturation,serum ferritin were measured.MHD patients were divided into adequacy group and non-adequacy group according to spKt/V value.Serum NGAL between two groups was compared and correlation of NGAL with spKt/V,inflammatory factors,and other clinical indexes was examined by Pearson,multivariate regression Logistic model and area under curve (AUC) of ROC.MHD patients were followed up for 3 months.Similar correlation analysis was carried out before and after follow up between two groups.Results Serum NGAL of MHD patients was higher compared to healthy people [(445.45±50.34) μg/L vs (50.02 ±6.45) μg/L].Among 150 MHD patients,95 were classified into adequacy group,while 55 into non-adequacy group according to spKt/V value.Serum NGAL was significantly higher in adequacy group as compared to non-adequacy group [(589.14±56.34) μg/L vs (360.13±46.23) μg/L,P＜0.05].Serum NGAL was positively correlated to spKt/V,CRP,TSAT [r=0.652,0.825,0.785,all P＜0.05],but not to serum ferritin.Serum NGAL was also correlated to spKt/V,CRP,TSAT by multivariate regression Logistic model.AUC showed that NGAL level could reflex the condition of dialysis adequacy.During the follow up,all adequacy patients remained the same adequacy status.Thirty-eight out of 55 non-adequacy patients were qualified for complete adequacy after intervention.The serum NGAL before and after intervention was (368.14±56.21) μg/L and (360.56±46.23) μg/L respectively without significant difference.Conclusions Serum NGAL is obviously higher in MHD patients with dialysis adequacy compared to those without dialysis adequacy.Serum NGAL is positively correlated to spKt/V,CRP,TSAT.NGAL can reflex the condition of dialysis adequate in MHD patients.
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ObjectiveTo observe the occurrence and related factors of pulmonary hypertension (PH) in maintenance hemodialysis (MHD) patients.MethodsOne hundred MHD patients were divided into PH group(41 cases) and non-PH group(59 cases) according to the pulmonary artery pressure measured by cardiac ultrasound.The age,systolic blood pressure (SBP),diastolic blood pressure(DBP),vascular access way (arteriovenous fistula,central venous catheter ),dialysis adequacy index (KT/V) and albumin (ALB),hemoglobin (Hb),calcium (Ca),phosphorus (P),parathyroid hormone (PTH) was compared between two groups,and related factors were analyzed by Logistic regression analysis.Results Pulmonary artery systolic pressure (PASP) of PH group and non-PH group was (46.72 ±9.26) mm Hg (1mm Hg =0.133 kPa) and (32.06 ±6.14) mm Hg,and the difference between two groups was significant (P＜ 0.01 ).The arteriovenous fistula rate accounts for 95.1％ (39/41)in PH group,and Hb was (78.27 ±4.53) g/L,PTH was (863.16 ± 146.91 ) ng/L,right ventricular cross diameter was(32.43 ±4.32) mm,right atrial cross diameter was (38.67 ± 7.13 ) mm,right atrial length diameter was (57.23 ± 7.26) mm and left ventricular ejection fraction (LVEF) was (56.38 ± 11.43 )％,while 79.7％ (47/59),(82.67 ± 5.32 ) g/L,(725.68 ± 137.14) ng/L,(21.64 ± 3.65 ) mm,(34.17 ± 6.28) mm,(46.18 ± 6.20) mm,(64.37 ± 6.83)％ in non-PH group,the differences were statisticaly significant between two groups(P ＜0.05 or ＜0.01).Multivariate Logistic regression analysis showed that serum PTH,Hb,rightatrial cross diameter,LVEF,vascular access way were related factors of PH.ConclusionThe serum PTH,Hb,right atrial cross diameter,LVEF,vascular access way are related factors of PH,and the PH may be early prevented and treated by correcting these related factors.
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The national standards of biofuels specify the technique specification and analytical methods. A water content certified reference material based on the water saturated octanol was developed in order to satisfy the needs of the instrument calibration and the methods validation, assure the accuracy and consistency of results in water content measurements of biofuels. Three analytical methods based on different theories were employed to certify the water content of the reference material, including Karl Fischer coulometric titration, Karl Fischer volumetric titration and quantitative nuclear magnetic resonance. The consistency of coulometric and volumetric titration was achieved through the improvement of methods. The accuracy of the certified result was improved by the introduction of the new method of quantitative nuclear magnetic resonance. Finally, the certified value of reference material is 4.76% with an expanded uncertainty of 0.09%.
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Biofuels , Reference Standards , Magnetic Resonance Spectroscopy , Methods , Octanols , Chemistry , Reference Values , WaterABSTRACT
OBJECTIVE:To study the hemostatic effect of reptilase on abdominal operations in perioperative period.METH-ODS:The study was conducted in 66 patients.They were randomly divided into 2 groups:reptilase group(n=35) and control group(n=31).In reptilase group,the reptilase was given in pre-operative,post-operative and intraoperative period and in control group,other commonly-used hemostants were given in the same way as in reptilase group.The amount of bleeding,the content of D-D in venous blood,the quantity of postoperative bloody drainage were compared between 2 groups.RESULTS:The amount of intraoperative bleeding,postoperative contents of D-D in venous blood and the quantity of bloody drainage in reptilase and control group were(312.1?30.3)ml,(521.5?29.2)ml(P
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0.05).Sexual dysfunction was 13.8% and 46.5% respectively,and was significant(P
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Objective:To investigate the expressive characters and their significance by detecting the expressions of HBsAg,Fas and proliferating cell nuclear antigen(PCNA) in both hepatocellular carcinoma(HCC) and their surrounding non-cancerous tissues.The correlation between HBV infection and HCC,and the possible mechanism of hepatocellular carcinogenesis were discussed.Methods:HBsAg,Fas and PCNA were detected by immunohistochemistry from 52 cases of hepatocellular carcinoma as well as 42 corresponding non-cancerous tissues.Clinical data suchas serum AFP levels was also analysed.Results:Both HBsAg and Fas were strongly expressed in non-cancerous tissues,with positive rate of each achieving 78.6%.However their expression rates in cancer tissues were 21.2% and 30.8% respectively,significantly lower and less intensive than that of their corresponding non-cancerous ones(P
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Objective:To detect the expression of 5-LOX and observe its inhibitory effect in humane pancreatic cancer cell.Methods:5-LOX mRNA and protein expression were analyzed by RT-PCR and immunocytochemical staining as well as images analyzing system,and the effect of MK886 was tested.Results:MK886 effectively down-regulated the expression of 5-LOX mRNA.Immunocytochemical staining and images analysis confirmed that the expression 5-LOX protein in cell treated with MK886 was lower than untreated control cell.Conclusion:Inhibitor of 5-LOX can effectively prevent the target mRNA into protein product,thus block or weaken the expression of 5-LOX.
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objective to find a proper pathway for the regional chemotherapy of pancreatic carcinoma. Method A total of 39 cases of unresectable pancreatic carcinoma was collected and divided into five groups. Ⅰ: celiac artery group, 5 cases; Ⅱ: gastroduo-denal artery group,24 cases;15 cases were directly inserted into this artery (ⅡB)and 9 cases indirectly through gastroepiploric artery (ⅡA). Ⅲ: gastroepiploric right artery group, Scases; Ⅳ: splenic artery group, 5 cases. Result. For these groups, the first group can be detected that the pancreatic head is clearly dyed; in the second group the pancreatic head was apparently dyed. The body and tail of pancreas were clearly dyed in the splenic artery group and failed in the second group. Conclusion For pancreatic head carcinoma, the gastroduodenal artery is the first choice; splenic artery is proper for the carcinoma in the body and tail of pancreas.
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Objective To investegate the influencing factors of intra-abdominal infectious complications after pancreatoduodenectomy(PD). Methods The postoperative intra-abdominal infectious complications occurrence rate and the influencing factors after PD in 196 patients in recent 7 years were retrospectively analyzed.Results After PD,the rate of postoperative intra-abdominal infections was significantly higher than the rate of other complications. The rate of intra-abdominal infections in patients with near normal pancreatic remnent and with pulmonary infections were significantly higher than those in with harder pancreatic remnant and without pulmonary infections(P