TRPV4 induces apoptosis via p38 MAPK in human lung cancer cells

Lung cancer is one of the most common cancers worldwide. TRPV4 belongs to the 'transient receptor potential' (TRP) superfamily. It has been identified to profoundly affect a variety of physiological processes, including nociception, heat sensation, and inflammation. Unlike other TRP superfamily channels, its roles in cancers are unknown. Here, we elucidated the effects of TRPV4 and molecular mechanisms in human lung cancer cells. The levels of TRPV4 were detected in human lung cancer tissues and the paired paracarcinoma tissues by real-time PCR and western blotting analysis. The proliferation of human lung cancer cells was determined by MTT assay. Cell apoptosis was determined by FACS assay. The results demonstrated that low levels of TRPV4 were detected in clinical lung carcinoma specimens. Over-expression of TRPV4 induced cell death and inhibited cell proliferation and migration in A549 cells and H460 cells. Moreover, over-expression of TRPV4 enhanced the activation of p38 MAPK signal pathway. Inhibition of p38 MAPK abolished the effects of TRPV4 on cell proliferation, apoptosis, and migration in A549 cells. Collectively, our findings indicated that TRPV4 induced apoptosis via p38 MAPK in human lung cancer cells and suggested that TRPV4 was a potential target for therapy of human lung cancers.

Paired observation on light-cured composite resin and nano-composite resin in dental caries repair

To compare the value of light-cured composite resin with that of nano-composite resin in dental caries repair. 88 patients taking dental caries repair in our hospital from May 2014 to April 2015 were selected, and divided into observation group and control group by coin method with 44 patients in each group. Nano-composite resin was used in observation group, while light-cured composite resin in control group. Then, the occurrence rates of odontohyperesthesia, aesthetic satisfaction with dental caries repair and complications were compared between two groups by visual analogue scale [VAS]. The occurrence rate of odontohyperesthesia in observation group is significantly lower than that in control group [9.09% [4/44] vs 31.82% [14/44], 6.82% [3/44] vs 22.73% [10/44], 2.27% [1/44] vs 13.64% [6/44]] with difference of statistical significance [P<0.05] 1 week, 4 weeks, and 8 weeks after repair. VAS score of patients in observation is significantly lower than that in control group [[2.78 +/- 0.56] scores vs [5.22 +/- 0.76] scores, [2.02 +/- 0.35] scores vs [4.32 +/- 0.57] scores, [1.12 +/- 0.14] scores vs [2.41 +/- 0.43] scores] 1 week, 4 weeks, and 8 weeks after repair. Moreover, the difference in comparison of interactive effects between two groups, between different time points, and between groups at different time points has statistical significance [P<0.05]. Nano-composite resin can lower the occurrence rate of odontohyperesthesia in dental caries repair, reduce the pain of patients, and improve the satisfaction of patients with tooth appearance