ABSTRACT
Objective To investigate the association of IL-10,IFN-γ gene polymorphisms at position-1082G/A and + 874A/T and susceptibility to pulmonary tuberculosis(PTB)through a case-control study.Methods Polymerase chain reaction with sequence specific primer(PCR-SSP)/sequencing method,single nucleotide polymorphisms(SNP)of IL-10 and IFN-γ gene at position-1082 and +874 were used on pulmonary tuberculosis patients(group PTB)and normal healthy subjects(group NHS)in the Han population from Shenzhen city of China.Results The genotype frequencies of A/A homozygotes,A/G heterozygous and G/G homozygous at-1082 site of IL-10 gene in patients with pulmonary tuberculosis were 85.4%,13.1%,1.5% respectively and A,G allele frequencies were 91.9%,8.1% respectively.In healthy subjects,the genotype frequencies of A/A homozygotes,A/G heterozygous and G/G homozygous were 77.5%,22.0%,0.5% respectively and A,G allele frequencies were 88.5%,11.5% respectively.Genotype distribution between the two groups was significantly different(P＜0.05).The genotype frequencies of A/A of IL-10 in the patient group were higher than that of control group.In contrast,the genotype frequencies of G/A in the control group were higher than that in the patient group(P＜0.05).There was no statistical difference found between A and G allele(P＞0.05).For IFN-γ(+874)and no significant difference was found on the distribution of genotypes or alleles between the two groups.Conclusion The IL-10(-1082)gene SNP might be associated with the susceptibility to tuberculosis.A/A genotype might be a risk factor for the susceptibility on tuberculosis while the SNP of cytokines(+874)might not be associated with the susceptibility to tuberculosis in Chinese Han populations.