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Chinese Journal of Oncology ; (12): 110-113, 2002.
Article in Chinese | WPRIM | ID: wpr-354057

ABSTRACT

<p><b>OBJECTIVE</b>To develop an oral DNA vaccine based on MG(7)-Ag mimotope of gastric cancer using attenuated Salmonella typhimurium and evaluate its efficacy and protective effect.</p><p><b>METHODS</b>The eukaryotic expression vector including the MG(7)-Ag mimotope and a Th epitope was constructed, and then transduced into an attenuated Salmonella typhimurium to get the oral DNA vaccine. C57BL/6 J mice were orally immunized with 1 x 10(8) cfu Salmonella transfectants, with Salmonella harboring empty plasmid, with phophate buffered saline (PBS) as control. At the 6th week, serum titer of MG(7) antibody was detected by ELISA. In the 8th week, a [(3)H]-thymidine incorporation assay was performed to test the proliferation of murine spleen cells to the stimulant of MG(7)-Ag mimicry peptide. At the same time, Ehrlich ascites carcinoma cells expressing MG(7)-Ag were used in tumor challenge assay to evaluate the protective effect of the immunization.</p><p><b>RESULTS</b>The oral DNA vaccine induced MG(7) antibody in mice, while in vivo unprimed proliferation assay of the spleenocytes showed no difference among the three groups. Two weeks after tumor challenge, 2 in 7 immunized mice were tumor free, while none in the control group was protected.</p><p><b>CONCLUSION</b>Oral DNA vaccine based on the MG(7)-Ag momitope is immunogenic. It is able to induce specific immunity response against tumor in mice, and the vaccine is partially protective.</p>


Subject(s)
Animals , Female , Mice , Administration, Oral , Amino Acid Sequence , Antigens, Neoplasm , Blood , Genetics , Allergy and Immunology , Base Sequence , Cancer Vaccines , Genetics , Allergy and Immunology , Therapeutic Uses , Epitopes , Genetics , Allergy and Immunology , Mice, Inbred C57BL , Molecular Mimicry , Genetics , Allergy and Immunology , Molecular Sequence Data , Plasmids , Genetics , Polymerase Chain Reaction , Stomach Neoplasms , Drug Therapy , Allergy and Immunology , Treatment Outcome , Vaccines, DNA , Genetics , Allergy and Immunology , Therapeutic Uses
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