ABSTRACT
Periodontitis is a chronic inflammatory disease that destroys the integrity of tooth-supporting tissue. Periodontitis is listed as a major oral disease by the World Health Organization and is a public-health problem affecting global oral and systemic health. The fourth national oral health epidemiological survey has revealed that periodontitis is one of the most common oral problems in China. With the development of science and medicine, increased attention is being paid to the importance of oral health and its influence on general health. Accordingly, stomatologists are required to master more relevant information on clinical diagnosis and treatment, as well as to pay more attention to the diagnosis and treatment methods of patients with different systemic diseases. This article expounds the diagnosis and treatment strategy of patients with systemic disease periodontitis. We aimed to help stomatologists make more reasonable diagnosis and treatment decisions.
Subject(s)
Humans , Periodontitis/therapy , Mouth Diseases , Oral Health , ChinaABSTRACT
Several types of molecules, including the small non-coding RNAs, are involved in the precision regulation of neural development. The expression of microRNAs appears in a specific spatialtemporal sequence during the neural development, and abnormal expression may lead to neural dysplasia. MicroRNAs also play important roles in the regulation of cell biological behaviors in terms of survival, proliferation and differentiation. Neural stem cells are the mother cell of the nervous system. The proliferation, differentiation and migration of neural stem cells are tightly controlled so as to generate appropriate number and phenotype of daughter cells to ensure normal neural development. MicroRNAs are involved in the regulation of neural stem cell proliferation and differentiation via affecting the expression and function of their target mRNAs.
Subject(s)
Animals , Humans , Cell Differentiation , Cell Proliferation , MicroRNAs , Physiology , Nervous System , Neural Stem Cells , Cell Biology , PhysiologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effects of curcumin against HeLa cell invasion and migration and explore the underlying mechanisms.</p><p><b>METHODS</b>HeLa cells were exposed to curcumin treatment at the concentrations of 0, 10, 25, 50, 100, 150 and 200 µmol/L for 24 h. MTT and TUNEL assays were used to assess the cell proliferation inhibition and apoptosis, respectively. Transwell assay was used to evaluate the invasiveness and migration of the treated cells, and RT-PCR and Western blotting were employed to detect the changes in the expression of inducible nitric oxide synthase (iNOS), and MMP-9 and E-cad, the 2 markers of cell invasion and migration, were detected by Western blotting. The capacity of NO production in HeLa cells was measured by Griess method.</p><p><b>RESULTS</b>Curcumin inhibited the proliferation of HeLa cells by inducing cell apoptosis in a concentration-dependent manner. Curcumin inhibited the invasion and migration of HeLa cells by increasing E-cad expression and decreasing MMP-9 expression, and also decreased the expression level of iNOS and NO production in the cells.</p><p><b>CONCLUSION</b>Curcumin inhibits the invasion and migration of HeLa cells by decreasing the expression of iNOS.</p>