Construction of phylogenetic tree and sequence analysis on BDV p24 gene in both livestock and host in Ningxia / 中华流行病学杂志

Objective To gain detailed insights into the Borna disease virus infection and its genetic characteristics and phylogeny in Ningxia,China.Methods BDV p24 segment were detected by fluorescence quantitative nested RT-PCR from peripheral blood mononuclear cells of 119 patients with viral encephalitis,205 cattle,978 sheep,46 patients with cerebrovascular diseases and 13 patients with multiple sclerosis.Data from phylogenetic analysis on BDV p24 positive samples together with those from the positive gene sequences of animals and patients in the previous studies in Ningxia,were compared to the 29 sequences provided by the GenBank from 7 animals in 5 countries.Both the sequence homologous similarity of the nucleotide and amino acid sequences were analyzed and gene phylogenetic tree was reconstructed.Results Data from 23 collections of positive test samples,together with the ones from early detection of gene sequence analysis,showed that the homologous similarity sequences of both nucleotide and amino acid was between 95.3% and 100.0% and highly homophylic with HE80 that detected from ill horses in Germany.One part of nucleotide sequences formed the 'Ningxia independent branch'while the other one belonged to the 'Germany-Ningxia-Japan mixed branch'.There was a high identity within the branch.Conclusion A Ningxia independent BDV strain from geographical origin might exist while the epidemic strains were imported with multiple sources.

Research on the association between Borna disease virus infection and the viral encephalitis / 中华流行病学杂志

<p><b>OBJECTIVE</b>To investigate the infection of Borna disease virus (BDV) in unidentified viral encephalitis patients in Ningxia in order to explore if the nucleotide sequence and amino acid sequence in BDV p24 were homophylic with the overseas standard strain. We also intended to investigate the correlation between BDV infection and the unidentified viral encephalitis patients from Ningxia to lay an experimental basis for etiological diagnosis, prevention and treatment on certain human neuropsychiatric disorders.</p><p><b>METHODS</b>BDV p24 gene fragment was detected by nested reverse transcriptase polymerase chain reaction with fluorescence quantitative PCR (FQ-nRT-PCR) in peripheral blood mononuclear cells (PBMCs). Samples were from 59 unidentified viral encephalitis patients and 60 normal controls. For those positive products, gene sequence and amino acid sequence were then analyzed by BLAST and DNAsist 5.0.</p><p><b>RESULTS</b>The positive rate of the BDV p24 gene fragment in PBMCs from the unidentified viral encephalitis (10.17%) was significantly higher than from the controls (0%) (P < 0.05). Data from the gene sequence on those positive products showed BDV p24 fragment in the patients with unidentified viral encephalitis from Ningxia was homophylic with strain H3915 detected from ill horses (97.67%), as well as with the strain H1766 (96.51%) and strain V (95.35%). However, their amino acid sequences remained the same.</p><p><b>CONCLUSION</b>BDV infection might probably have existed in the unidentified viral encephalitis patients of Ningxia. The gene sequence seemed to be homophylic with that of standard strain H1766 and strain V, especially with strain H3915.</p>

Study on molecular epidemiology of Borna disease virus in Ningxia and vicinal regions / 中华流行病学杂志

<p><b>OBJECTIVE</b>In order to investigate the epidemics of borna disease virus (BDV) in Ningxia and its vicinal regions.</p><p><b>METHODS</b>p24 fragment of BDV from: (1) peripheral blood mononuclear cells (PBMC) and cerebrospinal fluid mononuclear cells (CSFMC) from 52 patients with viral encephalitis (VE) and 32 healthy donors, (2) peripheral blood mononuclear cells (PBMC) from 53 patients with depressive disorder (DD) and from 360 sheep, were examined by nested reverse transcriptase polymerase chain reaction(PCR) with fluorescence quantitative PCR. Gene sequence and amino acid sequence were analysed for positive product and the molecular epidemiologic characteristics by drawing phylogenetic trees.</p><p><b>RESULTS</b>The positive rate of BDV p24 in CSFMC from VE (11.54%) and in PBMC from DD 11.32% was significantly higher than that in healthy donors (0%) (P < 0.05). The phylogenetic trees indicating the genetic relationship of the p24 fragment of BDV in both sheep and VE, DD in China and was similar to the nucleotide sequence of H1766 strain in Germany.</p><p><b>CONCLUSION</b>Data indicated that the BDV infection was possibly existing in VE, DD patients and health sheep in Ningxia and its vicinal regions with confined locality which called for further study.</p>

Immune Responses of Th1/Th2 in Children with Mycoplasma Pneumoniae Pneumonia / 实用儿科临床杂志

Objective To study the immune responses of Th1 and Th2 subsets of T cells in 26 children with mycoplasma pneumo-niae pneumonia(MPP).Methods ELISA was used to detect the levels of interferon- ?( IFN- ?) and interleukin-4(IL-4) in the serum in 12 healthy children as normal controls and 26 patients of acute stage as acute stage MPP group, 9 of whom in recovery stage were as recovery stage MPP group. Results IFN - ? level in acute stage MPP group was significant higher than that in normal controls (P 0.05); and IFN - 7/IL - 4 ratio was significant higher than that in normal controls( P0.05).Conclusion Thl responses increase and Th2 responses decrease in children with MPP,and this kind of response persists during the recovery stage.

Significance of Interleukin-10 and Transforming Growth Factor-beta 1 Detection in Children with Mycoplasma Pneumoniae Pneumonia / 实用儿科临床杂志

Objective To explore the changes of interleukin-10(IL-10) and transforming growth factor-beta 1(TGF-?1)in 26 children with mycoplasma pneumoniae pneumonia(MPP).Methods Enzyme-linked immunosorbent assay(ELISA) was used to detect the levels of IL-10 and TGF-?1 in the serum of 12 healthy children as normal controls and 26 patients of acute stage as acute stage MPP group,9 of acute stage MPP group in recovery stage as recovery stage MPP group.The levels of IL-10 and TGF-?1 were compared between the three groups.Results IL-10 level in acute stage MPP group was significantly lower than that in normal controls(P