ABSTRACT
Objective To discussi the feasibility and indication of selective primary suture after choledo-chotomy. Methods The selected patients were intra-operatively randomized to have either primary suture or T tube drainage after choledochotomy. Comparison of the clinical effects in different groups was done. Re-sults Totally 161 cases were randomized into two groups: the primary sutures group 85 cases and the T tube drainage group 76 cases. All patient had no serious or long-term complications. The average hospital stay in primary suture group was significantly shorter (8.5±3.2) d than T-tube group(16.3±6.5) d. Conclusion Under strict indications, the primary suture after choledochotomy is feasible, and has advantages compared with the traditional method.
ABSTRACT
The genetic indentification of 16S rDNA or ITS, capability of phosphate-solubilization and pH of medium, and optimization of medium of some microorganism isolated from mangrove were investigated in this study. The result showed that the fungi normally had much higher capacity to dissolve the inorganic phosphate than the bacteria, the capacity of the fungi was closely correlated to the pH of medium, but the relationship was weak for the bacteria. It was illustrated by single factor experiments that the optimal carbon and nitrogen sources were maltose and urea respectively. The orthogonal design was employed in testing the optimum composition of medium composed of 5 g/L maltose, 0.05 g/L urea, 5 g/L NaCl, pH 5. In this optimal medium, the effectively enrichment of bacteria could reach up to 6.06?109 CFU/mL under 30?C for 48 hours cultivation.
ABSTRACT
To observe the germistatic and germicidal effects of origanum volatile oil (OVI) on the dysentery bacteria, the abdominal cavity of mice was infected with Shigella sonne (Sh. sonnei) and Shigella flexneri (Sh. flexneri) F2a. After OVI was given to the mice via gastric lavage, the effects of OVI on the infected mice were observed. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for dysentery bacteria were determined in vitro. The results showed that origanum volatile oil showed obvious protective effect on mice infected with Sh. sonnei and Sh. flexneri F2a and it had germistatic and germicidal effects on dysentry bacteria. We are led to conclude that origanum volatile oil is an effective medicine against the infection of dysentery bacteria.
Subject(s)
Anti-Bacterial Agents/pharmacology , Dysentery, Bacillary/microbiology , Microbial Sensitivity Tests , Oils, Volatile/pharmacology , Shigella flexneri/drug effects , Shigella flexneri/isolation & purification , Shigella sonnei/drug effects , Shigella sonnei/isolation & purificationABSTRACT
To observe the germistatic and germicidal effects of origanum volatile oil (OVI) on the dysentery bacteria, the abdominal cavity of mice was infected with Shigella sonne (Sh. sonnei) and Shigella flexneri (Sh. flexneri) F2a. After OVI was given to the mice via gastric lavage, the effects of OVI on the infected mice were observed. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for dysentery bacteria were determined in vitro. The results showed that origanum volatile oil showed obvious protective effect on mice infected with Sh. sonnei and Sh. flexneri F2a and it had germistatic and germicidal effects on dysentry bacteria. We are led to conclude that origanum volatile oil is an effective medicine against the infection of dysentery bacteria.
Subject(s)
Animals , Female , Male , Mice , Anti-Bacterial Agents , Pharmacology , Dysentery, Bacillary , Microbiology , Microbial Sensitivity Tests , Oils, Volatile , Pharmacology , Shigella flexneri , Shigella sonneiABSTRACT
Object To apply the technique of tissue culture to the rapid propagation of Stemona japo-nica (Bl.) Miq. and lay a foundation for the rapid propagation of its improved breeds. Methods The explants used for culture were stem segments with axillary bud of S. japonica. The media were MS basal media containing different plant hormones. Results The results of the study indicated that shoots could be induced successfully in MS medium supplement with 6-BA 3.0 mg/L and IBA 0.2 mg/L, and the better medium for bud multiplication and root inducement were MS+6-BA 3.0 mg/L+IBA 0.3 mg/L and MS+IBA 2.0 mg/L+AgNO 3 0.5 mg/L respectively. After 30 days' culture, the rate of rooting reached over 50%. The survival rate of transplanting was raised obviously by using 20 mg/L carbendazim treatment. Conclusion The primary plant regeneration system of S. japonica has been established, which makes the industrial production possible.