ABSTRACT
【Objective】 To analyze the commonality and characteristics between voluntary blood donors and hematopoietic stem cell donors in this region, and explore the potential for integration and development between China Marrow Donors Program (CMDP) and voluntary blood donors, especially platelet donor databases, so as to improve recruitment success rate and inventory rate. 【Methods】 The database modeling and comparison methods were used to screen and stratify the matching and integration degree between the voluntary blood donors in recent 10 years and the marrow donors in the Shaanxi Branch of CMDP. The frequencies of HLA-A,-B alleles, HPA alleles and haplotypes were calculated with Arlequin 3. 5. 2. 2 software, and the matching probability of different platelet donor reserve pools was conducted according to the phenotypic frequencies. 【Results】 Among the voluntary donors with known HLA genotypes in this region, according to their blood donation behavior,the active blood donors excavated were divided into the first, second, third and fourth echelons of platelet donor reserve pools, with 696, 2 752, 9 092 and 12 028 donors, respectively. The first echelon had the highest proportion of 10-50 times of platelet donations and 10-20 times of whole blood donations, with 13.65% and 26.01%, respectively. The second echelon had 10-20 times of whole blood donations and 10-50 times of platelet donations, accounted for 15.04% and 1.38%, respectively, which were significantly different from other echelons' blood donation characteristics (P<0.05). With a database size of the existing platelet donor bank adding the first and second echelons (n=4 955), there was a 69.02% probability of matching at least one donor with matching HLA-A-B phenotype. When considering the matching ABO and HPA phenotypes, the probability of finding at least one donor with fully matching HLA, HPA and ABO isotype (type B as an example) was 48. 73%. 【Conclusion】 The three groups of whole blood donation, apheresis platelet donation and marrow donation in Xi'an area have a large cross-distribution. Compared with expanding the storage capacity from scratch, the active blood donors in CMDP database are the largest back-up force of platelet donors. While expanding the effective storage capacity, it can minimize the cost of building platelet donor bank and the demand for resources.
ABSTRACT
ObjectiveTo summarize the method and experience of the model of rat heterotopic heart transplantation and to improve the rate of success of the experiment.Methods120 heterotopic heart transplantations were performed by Ono's way,including 60 prepared transplantations and 60 formal transplantations.ResultsRate of success of prepared transplantations was 45％,and formal transplantation was 85％.Mean time of formal operation was (74.3±17.1)min,removal time of the donor heart was (7.9±4.2)min,ischemia time was (29.6±10.3) min and the survival time of allograft was (11.1±5.7)days.5 of the recipients had a long-term survival,and the maximum weight was 740 g.ConclusionsGood surgical technique and rigorous perioperative management is very important in setting up the rat model of heterotopic heart transplantation.
ABSTRACT
Iontophoresis possesses the unique superiority as an effective way for promoting the permeability in a therapy system which gives medicine through human' skin directly.It has been developing very quickly in recent years.The application of DC,pulse DC and the electroporation causes the flux greatly enhanced,especially the application of electroporation which can fast reduced the skin resistance and enhance the flux for its reversible function to the skin.What's more,there has been an improved way for iontophoresis now,which uses the AC with an offset of DC.This method can stabilize the skin resistance so as to stabilize the human epidermis membrane parameters,including the pore area,the pore distribution,the pore surface charge density,thus keeping the flux constant.
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Along with the development of society,people pays more attention to health.Since water resources is increasingly missing with depravation,the water quality analysis technique gets a value increasingly,which promotes the development of portable water quality instrument.This paper expounds the present situation and future prospects of portable water quality instrument,puts forward its application in army,aquiculture and enviroment,and enumerates the main technique parameters of several instruments both here and abroad.
ABSTRACT
Processed by the MATLAB-based wavelet analysis,normal EEG signal is used as stimulating source to stimulate brain at proper points for insomnia treatment.This paper explains the design principle of the instrument as well as the hardware structure and the software implementation.