ABSTRACT
Ginsenoside Rg1, one of the most notable active components of Panax ginseng, has been widely reported to exert anti-inflammatory actions. This study aimed to reveal whether ginsenoside Rg1 also exhibits beneficial roles against lipopolysaccharide (LPS)-induced apoptosis and inflammation in human renal tubular epithelial cells, and to evaluate the potential role of the component on tubulointerstitial nephritis treatment. HK-2 cells were treated with various doses of ginsenoside Rg1 (0, 50, 100, 150, and 200 μM) in the absence or presence of 5 μg/mL LPS. Thereafter, CCK-8 assay, flow cytometry, western blot, migration assay, reactive oxygen species (ROS) assay, and ELISA were carried out to respectively assess cell viability, apoptosis, migration, ROS activity, and the release of inflammatory cytokines. As a result, ginsenoside Rg1 protected HK-2 cells from LPS-induced injury, as cell viability was increased, cell apoptosis was decreased, and the release of MCP-1, IL-1β, IL-6, and TNF-α was reduced. Ginsenoside Rg1 functioned to HK-2 cells in a dose-dependent manner, and the 150 μM dose exhibited the most protective functions. Ginsenoside Rg1 had no significant impact on cell migration and ROS activity, while it alleviated LPS-induced ROS release and migration impairment. Furthermore, the down-regulations of p-PI3K, p-AKT, and up-regulations of PTEN, p-IκBα, p-p65, Bcl-3 induced by LPS were recovered to some extent after ginsenoside Rg1 treatment. In conclusion, ginsenoside Rg1 protects HK-2 cells against LPS-induced inflammation and apoptosis via activation of the PI3K/AKT pathway and suppression of NF-κB pathway.
Subject(s)
Humans , Lipopolysaccharides , Apoptosis/drug effects , Ginsenosides/pharmacology , Epithelial Cells/drug effects , Kidney Tubules/cytology , Anti-Inflammatory Agents/pharmacology , Enzyme-Linked Immunosorbent Assay , Cell Line , Cell Survival/drug effects , Blotting, Western , Reproducibility of Results , Analysis of Variance , Cytokines/analysis , Cytokines/drug effects , Cell Migration AssaysABSTRACT
L'amenorrhee secondaire (A.S.) chez les femmes est tres frequente. Mais l'etiologie reste inconnue. Les auteurs ont pris 63 cas de femmes presentant l'A.S. et ont fait le dosage en oestrogene de l'epithelium exofoliatif du vagin. L'utilisation d'oestrogene et du progesterone reste donc tres importante dans le traitement de l'amenorrhee secondaire; l'apport du calcium et de la vitamine D peut prevenir ou diminuer le risque d'une osteoporose
Subject(s)
Amenorrhea , Estrogens/therapeutic use , Progesterone/therapeutic useABSTRACT
La Mission Medicale Chinoise travaille a la Clinique Bon Secours; annexe du C.H.U. Tokoin de Lome depuis 21 ans. Une statistique des consultations dans le service de Gynecologie sur la periode allant de janvier 1988 a janvier 1995 donne un nombre total de malades consultees egal a 31.102. Parmi les affections frequemment diagnostiquees; la pelvite vient en tete avec 9.984 cas; soit 32.10 pour cent. La sterilite essentiellement provoquee par l'obstruction des trompes dues a la pelvite; vient en seconde position avec 6.784 cas (soit 27;83 pour cent) dont 6.140 cas (19;74 pour cent) sont dus a l'obstruction des trompes consecutive a la pelvite. La pelvite et les maladies resultant d'elle representent ainsi 51;84 pour cent du total sus mentionne. C'est donc une maladie frequente; menacant gravement la sante des Togolaises