ABSTRACT
This study aims to analyse the potential relationship between single-nucleotide polymorphism (SNPs) in trace element related metabolic genes GSTM3, GSTP1, GPX1 and NKG2D and the risk of gastric cancer. A case–control study was conducted in the hospital of Xianyou, Fujian, China. In this study, a total of 299 patients with histopathological diagnosis in gastric cancer and 295 healthy control subjects were involved. Association between the SNPs in trace element-related metabolic genes and gastric cancer risk was analysed using the unconditional logistic regression model. No relationship was found between the SNPs of GSTM3 and GPX1 genes and gastric cancer risk. However, the risk of gastric cancer is related to the SNPs of NKG2D gene (rs1049174). Patients who carry the rs1049174 GG genotype have a higher incidence of the gastric cancer and a multivariate odds ratio (OR) of 1.85 (95% confidence intervals (CI): 1.02–3.38). Through haplotype analysis, two haplotypes (i.e. A_rs1746123-T_rs10431294-G_rs1049174 and T_rs1746123-T_rs10431294-C_rs1049174), OR of 0.29 (95% CI: 0.15–0.56) and 0.33, (95% CI: 0.22–0.50), respectively, were found to have lower incidence of gastric cancer. Meanwhile, another two haplotypes (T_rs1746123-C_rs10431294-C_rs1049174 and T_rs1746123-T_rs10431294-G_rs1049174), OR of 1.81 (95% CI: 1.40–2.34) and 3.09 (95% CI: 2.30–4.16), respectively, were found to have a higher incidence of gastric cancer. Further, no influence of the haplotype on the risk of cardia gastric cancer was found. However, the haplotype T_rs1746123-T_rs10431294-C_rs1049174 had lower incidence of noncardia gastric cancer by 46%. Our data showed that polymorphisms of trace element-related metabolic genes are important in gastric cancer pathology.
ABSTRACT
We conducted a case-control study to investigate the association between dietary folate, vitamin B[6] and vitamin B[12] intake, MTHFR and MTR genotype, and breast cancer risk. Genotyping for MTHFR C677T and A1298C and MTR A2756G polymorphisms were performed using polymerase chain reaction-restriction fragment length polymorphism analysis [PCR-RFLP] method. The intake of folate, vitamin B[6] and vitamin B[12] were calculated by each food item from questionnaire. Subjects with breast cancer tended to have more first-degree relatives [X[2]=30.77, P < 0.001] and have high intake of folate [t=2.42, P=0.008] and Vitamin B[6] [t=2.94, P=0.002]. Compared to the reference group, women with MTHFR 677 TT genotype and T allele had a significantly increased risk of breast cancer, with ORs [95%CI] of 1.8[1.08-2.27] and 1.39[1.02-1.92], respectively. For those who had folate intake < 450 ug/day, MTHFR 667TT genotype was associated with a higher risk of breast cancer [OR=2.45, 95% CI=1.09- 5.82, P=0.02]. Similarly, subjects with Vitamin B[6] intake < 0.84 mg/day and MTHFR 667T allele genotype was correlated with a marginally increased risk of breast cancer. A significant interaction was observed between MTHFR C667T polymorphism and folate intake on the risk of breast cancer [P for interaction was 0.025]. This case-control study found a significant association between MTHFR C667T polymorphism, folate intake and vitamin B[6] and breast cancer risk, and a significant interaction was observed between MTHFR C667T polymorphism and folate intake on the risk of breast cancer