ABSTRACT
Aim To investigate the effects of acteoside (AS) on BRAIN AKT and NFkB in APP/PS1 double transgenic mice. Methods Fifty healthy APP/PS1 transgenic mice, half male and half female, and 10 control C57 mice were given the drug by gavage for 60 consecutive days. During the period of administration, and new object recognition experiments were conducted to detect the expression of AKT and NFkB related proteins in the hippocampus and cortex of the mice. Results Compared with model group, AS could significantly improve the nesting behavior of mice and en- hance the interest of exploring new objects in mice with Alzheimer' s disease. At the same time, compared with model group, AS significantly reduced the ratio of NFkB p-p65 /NFkB p65 in hippocampus and cortical tissues, and increased the ratio of p-AKT-308 /AKT and p-AKT473 /AKT. Conclusions AS may inhibit the apoptosis of nerve cells and protect nerve cells through the regulation of AKT and NFkB to treat neurodegenerative diseases.
ABSTRACT
OBJECTIVE： To optimize the extraction technology of verbascoside from Cistanche tubulosa， and to provide reference for further development and comprehensive utilization of C. tubulosa. METHODS： The content of verbascoside in C. tubulosa was determined by HPLC. The determination was performed on Inertsil-ODS-3V column with mobile phase consisted of methanol-0.2％ formic acid aqueous solution （40 ∶ 60， V/V） at the flow rate of 1 mL/min. The column temperature was 30 ℃， the detection wavelength was 330 nm， and the sample size was 10 μL. Using extraction rate of verbascoside as index， soaking time， ethanol concentration， liquid-solid ratio， extraction time and extraction times were investigated by single factor tests. According to the results of above tests， ethanol concentration， liquid-solid ratio and extraction time were optimized by Box-Behnken response surface methodology. The verification tests were carried out on the optimized extraction technology. RESULTS： The linear range of verbascoside was 18.65-932.4 μg/mL. The optimal extraction technology included that ethanol concentration 63％， liquid-solid ratio 8 ∶ 1 （mL/g）， soaking for 2 h， extraction time 1.5 h， extracting for 2 times. The extraction rates of verbascoside in the three parallel verification tests were 78.21％， 76.95％， 79.34％， respectively. The relative errors of those to predicted value 76.76％ were 1.89％， 0.25％， 3.36％. CONCLUSIONS： The optimized extraction technology of verbascoside from C. tubulosa is stable and feasible， and is suitable for the extraction of verbascoside.