ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of angiotensin II (Ang II) on pregnancy-associated plasma protein A (PAPP-A) and insulin-like growth factor 1 (IGF-1) mRNA expressions in human umbilical artery smooth muscle cells (HUVSMCs).</p><p><b>METHODS</b>In the presence or absence of Ox-LDL, HUVSMCs were cultured with Ang II of 10(-5) mol/L for 0, 6, 12, 24, 36, and 48 h, or with Ang II at the concentrations of 10(-7), 10(-6), 10(-5), and 10(-4) mol/L for 24 h, after which the cells were then collected to detect PAPP-A and IGF-1 mRNA expressions in the cells using RT-PCR.</p><p><b>RESULTS</b>At the concentration of 10(-5) mol/L, Ang II showed a time-dependent effect in inducing PAPP-A and IGF-1 mRNA expressions, which began to increase at 12 h of culture and reaching the highest level at 24 h. Ang II also dose-dependently induced PAPP-A and IGF-1 mRNA expressions, and 10(-5) mol/L Ang II induced the highest expression levels of the two genes. Ox-LDL exposure significantly further increased the expression levels of PAPP-A and IGF-1 mRNA in the cells regardless of the Ang II concentration or duration for cell treatment (P<0.05).</p><p><b>CONCLUSION</b>Ang II can time- and dose-dependently induces PAPP-A and IGF-1 mRNA expression in HUVSMCs and is responsible for inducing platelet activity and inflammatory reaction in acute coronary syndromes, and the effects of Ang II can be enhanced by Ox-LDL.</p>
Subject(s)
Humans , Angiotensin II , Pharmacology , Cells, Cultured , Drug Synergism , Insulin-Like Growth Factor I , Genetics , Metabolism , Lipoproteins, LDL , Pharmacology , Myocytes, Smooth Muscle , Cell Biology , Metabolism , Pregnancy-Associated Plasma Protein-A , Genetics , Metabolism , RNA, Messenger , Genetics , Metabolism , Umbilical Arteries , Cell Biology , MetabolismABSTRACT
Objective To discuss pedicle screw system in the treatment of unstable thoracolumbar fraetures of the results of surgical treatment.Methods Pedicle screw system for thoracolumbar unstable fracture of 46 cases.Results 39 cases were followed up for an average of 17 months after the intervertebral space height and thoracolumbar physiological curvature satisfactory recovery.The average Cobb angle and the average spinal stenosis index has been markedly improved,Frankel raise a grade:three.Conclusion Posterior short section of the pediele screw system is the treatment of thoracic and lumbar fractures of the effective ways is satisfied with the reduction,reliable fixation.
ABSTRACT
<p><b>OBJECTIVE</b>To study single nucleotide polymorphism (SNP) of all the coding region in ABCA1 gene in 112 patients with coronary heart diseases.</p><p><b>METHODS</b>With polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) combining argentation and glue retrieval, DNA sequencing, and restriction fragment length polymorphism (RFLP), the SNP of the 50 exons in all the coding regions of ABCA1 gene was detected in 112 patients with established diagnosis of coronary heart disease.</p><p><b>RESULTS</b>In the Chinese population with coronary heart disease, besides the SNP variation at R219K and M883I as widely reported, a new single base variation at A1092G in exon 7 was detected, which led to a conversion of the amino-side residue to M223V. This variation was confirmed to represent a novel SNP by RFLP in 108 normal subjects.</p><p><b>CONCLUSIONS</b>The Chinese population with coronary heart disease has not only the reported SNP changes at the sites R219K and M883I, but also changes at the new SNP site of M233V, which is discovered for the first time in M233V of ABCA1 gene. This variation may increase the risks for coronary heart diseases, and its exact function awaits examination in further epidemiologic survey.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , ATP Binding Cassette Transporter 1 , ATP-Binding Cassette Transporters , Genetics , Base Sequence , Coronary Disease , Genetics , Molecular Sequence Data , Open Reading Frames , Genetics , Point Mutation , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Polymorphism, Single Nucleotide , Polymorphism, Single-Stranded Conformational , Sequence Analysis, DNAABSTRACT
<p><b>OBJECTIVE</b>To identify ATP-binding cassette transporter A1 (ABCA1) gene polymorphism in Chinese patients with coronary artery disease (CAD).</p><p><b>METHODS</b>Single Nucleotide Polymorphisms in the ABCA1 gene were detected by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP)-DNA sequence and restriction-fragment length polymorphism (RFLP) method in 112 patients with CAD.</p><p><b>RESULTS</b>A novel polymorphism in the ABCA1 gene was found in two patients: M233V which exists in exon7 of ABCA1 gene and it's cDNA location is A1092G and converse 233 amino acid from Methionine to Valeric. We further collected the blood samples from 16 family members of one proband and M233V polymorphism was found in 5 out 16 family members.</p><p><b>CONCLUSION</b>M233V is a novel polymorphism in the ATP-binding cassette transporter A1 gene and this AG genotype had family proneness.</p>